I-RT Easyᵀᴹ II Master Premix yokuqala-strand cDNA synthesis yeXesha lokwenyani PCR
Inkcazelo yeKit:
- Inkqubo esebenzayo yokukhuphela umva, kuthatha imizuzu engama-20 kuphela ukugqiba udityaniso lwe-cDNA yomtya wokuqala.
-I-high-sensitivity reverse transcription system, i-pg-level templates inokufumana i-cDNA ephezulu.
-Isistim yokukhutshelwa umva inozinzo oluphezulu lwe-thermal, iqondo lobushushu lenkqubo linokuba phezulu ukuya kuma-50 ℃, kwaye inomsebenzi olungileyo wokukhutshelwa umva.
- Nkqu neesampulu ze-RNA ezingcolileyo (utywala ukuya kwi-60%, ityuwa ye-guanidine ukuya kwi-750mM) inokuthi ifakwe kwi-reverse transcription reaction.
- 2× RT OKANYE-EasyTM Umxube wongeze iiprimer ezikhutshelweyo eziphuculweyo (Random Primer, Oligo(dT)18 Isiqalo).
Iinkcazelo Kit
Foregene kaThixo 2× RT OKANYE-EasyTMUmxube unokudibanisa i-cDNA yokuqala-strand kwiqondo lokushisa elifikelela kuma-50 ° C, elilungele ukuphendulwa kwe-reverse transcription ye-complex complex structure RNA templates.
I-RNA ehlanjululweyo kwilabhoratri ihlala iqulethe i-alcohol kunye ne-guanidine iintsalela zetyuwa, ezineepropati ezinamandla zokuthintela kwi-reverse transcriptases, ezikhokelela kwimiphumo enganelisiyo yokukhuphela i-reverse okanye ukusebenza kakuhle kwe-reverse transcription.I-Foregene 2 × RT Mix ibonisa ukunyamezela okuphezulu kakhulu kutywala kunye netyuwa ye-guanidine.Ukunyamezela okuphezulu kotywala kwiisampuli ze-RNA yi-60%, kwaye ukunyamezela okuphezulu kwi-guanidine ityuwa yi-750mM.Nokuba i-RNA engacocekanga ingasetyenziselwa ukuguqulela umva ngokuphendula ngeForegene 2× RT OR-EasyTMXuba.
2 × RT OKANYE-EasyTMUmxube yinto ekhawulezayo yokukhuphela umva ireyinti ephuhliswe ngokukodwa i-PCR yexesha lokwenyani.Le sistim inobuchule obuphezulu bokukhutshelwa umva kwaye ayifuni kongezwe naziphi na iiprimers, kwaye ingenza uguqulelo oluhle lokukhuphela umva kwixabiso elincinci letemplates zeRNA.Inkqubo yokusabela eyodwa yenza ukusabela kwe-RT kube lula, ngokukhawuleza kwaye kusebenze ngakumbi.I-strand yokuqala ye-cDNA synthesis ingagqitywa kwimizuzu engama-20.Le mveliso inokusetyenziswa ngokudityaniswa ne-Foregene's fluorescent quantitative product Real Time PCR EasyTMukufumana iziphumo zovavanyo ezikumgangatho ophezulu.
Iinkcukacha
| RT EasyTMII I-Master Premix ye-first-strand cDNA synthesis ye-Real Time PCR | |||
| Amacandelo ekhithi | RT-01021 | RT-01022 | RT-01023 |
| 50 T (10μl inkqubo) | 200 T(10μl inkqubo) | 800 T (10μl inkqubo) | |
| 2 × RT OKANYE-EasyTMXuba | 0.25ml | 1ml | 1ml × 4 |
| RNase-Free ddH2O | 1.7ml | 1.7ml | 1.7ml × 3 |
| Incwadi Yemiyalelo | Iqhekeza eli-1 | Iqhekeza eli-1 | Iqhekeza eli-1 |
Iimpawu&izinto eziluncedo
- Inkqubo esebenzayo yokukhuphela umva, kuthatha imizuzu engama-20 kuphela ukugqiba udityaniso lwe-cDNA yomtya wokuqala.
-I-high-sensitivity reverse transcription system, i-pg-level templates inokufumana i-cDNA ephezulu.
-Isistim yokukhutshelwa umva inozinzo oluphezulu lwe-thermal, iqondo lobushushu lenkqubo linokuba phezulu ukuya kuma-50 ℃, kwaye inomsebenzi olungileyo wokukhutshelwa umva.
- Nkqu neesampulu ze-RNA ezingcolileyo (utywala ukuya kwi-60%, ityuwa ye-guanidine ukuya kwi-750mM) inokuthi ifakwe kwi-reverse transcription reaction.
- 2× RT OKANYE-EasyTMUmxube wongeze iiprimer ezikhutshelweyo eziphuculweyo (Random Primer, Oligo(dT)18Isiqalo).
Isicelo seKit
-Isetyenziswa ngokuthe ngqo kwi-Real Time PCR ukuhlalutya ngokobungakanani ukubonakaliswa kofuzo
- Inokukhawuleza nangokuchanekileyo ukuhlalutya amanani amancinci e-RNA njengeentsholongwane ze-RNA
-Reverse transcription ye RNA templates with high GC content or complex complex structure
Ukuhamba komsebenzi
Ukugcinwa kunye nobomi beshelufu
1. Iimeko zothutho
Yonke inkqubo yokuthutha i-ice box yobushushu obuphantsi, ukuqinisekisa ukuba ikiti ikwimo ye-<4 °C.
2. Iimeko zokugcina
RT EasyTMII igcinwa kwi -20°C.Gcina imveliso kwifriji ehlala rhoqo kwi-20 ° C ngokukhawuleza emva kokufunyanwa.Ukuba iimeko zokugcina zifanelekile, imveliso ayiyi kuthoba nayiphi na intsebenzo ngexesha le-1 leminyaka yokuqinisekisa.
