Mouse Umsila DNA Mini Kit Genomic DNA Extraction Kits ukusuka Mouse Umsila
Inkcazelo yeKit:
Eyona khithi ikhawulezayo yehlabathi yokukhupha umsila wemouse i-genomic DNA, enokukhupha i-DNA ye-genomic ekumgangatho ophezulu kumsila wempuku kwiyure enye.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
Inkcazo
Ngenxa yesakhiwo kunye neepropati zomsila wegundane ngokwawo, ukutsalwa komsila wemouse i-DNA ye-genomic isoloko ikhathaza abaphandi: enye kukuba ixesha lokucubungula umsila wemouse lide kwaye i-DNA ye-genomic iyancipha ngokulula, enye inzima ukuyiphatha kwaye ukucoceka kwe-DNA ye-genomic ekhutshiweyo iphantsi.
Le khithi isebenzisa i-DNA-Only Column enokuthi ibophe ngokukodwa i-DNA, i-Foregene Protease entsha kraca kunye nenkqubo ye-buffer ekhethekileyo, enokwetyisa iisampulu zomsila wempuku kwimizuzu engama-45, ngaloo ndlela inciphisa ukuthotywa kwe-DNA ye-genomic kunye nokunciphisa ixesha lokucubungula isampuli.Ikiti inokukhupha i-10-20μg ye-DNA ye-genomic ephezulu, i-high-purity-purity ukusuka kwi-0.5-1cm yolutsha okanye umsila wegundane omdala ngaphakathi kwe-60-90min.
I-DNA-kuphela kwe-silica gel matrix impahla esetyenziswe kwikholamu ye-spin yinto entsha ekhethekileyo yenkampani, enokuthi ibhengeze ngokufanelekileyo kwaye ibhengeze i-DNA.Umthamo omkhulu we-adsorption we-DNA yi-80μg.Inkqubo ekhethekileyo ye-buffer kunye ne-elution inokwandisa ukususwa kwe-RNA, iiprotheni ezingcolileyo, ii-ion kunye nezinye izinto eziphilayo kwiseli.I-fragment ye-DNA ye-genomic ekhutshweyo inkulu, iphakamileyo ekuhlanzekeni, izinzile kwaye inokwethenjelwa kumgangatho, kwaye ubukhulu beqhekeza le-DNA buzinzile malunga ne-23kb.
Ikiti ingasetyenziselwa ukukhupha ezinye izicubu zemouse ngaphandle komsila wemouse, kubandakanywa iindlebe zemouse, i-mouse muscle, isibindi segundane kunye nezinye izicubu, ukwenzela ukuba ikiti enye ingasetyenziselwa iinjongo ezininzi kwaye ihlangabezane neemfuno zabasebenzisi kwiimvavanyo ezahlukeneyo.
Iinkcukacha
50 Preps, 100 Preps, 250 Preps
Amacandelo ekhithi
| Isithinteli TL1 |
| Isithinteli TL2* |
| Isithinteli PW* |
| Buffer WB |
| Isithinteli EB |
| Foregene Protease Plus |
| Ikholamu yeDNA-Kuphela |
| Imiyalelo |
Iimpawu&izinto eziluncedo
-Akukho ungcoliseko lwe-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
-Isantya esikhawulezayo: I-Foregene Protease Plus inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye igaya iisampuli zezicubu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-40.
-Elula: I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ye-centrifugation ephantsi okanye i-ethanol precipitation ye-DNA.
-Ukhuseleko: Akukho kukhutshwa kwe-organic reagent efunekayo.
-Umgangatho ophezulu: I-DNA ye-genomic ekhutshweyo inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
Isicelo seKit
Ilungele ukutsalwa kunye nokuhlanjululwa kwe-DNA ye-genomic ukusuka kwimisila emitsha okanye ekhenkcezisiweyo enkulu kunye neyempuku (ekwasetyenziselwa ukuhlambulula i-mouse okanye i-rat muscle kunye ne-mouse ear genomic DNA).
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
-Le khithi ingagcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo kwiqondo lokushisa (15-25 ° C);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8 ° C.
Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yitshise kwindawo yokuhlambela amanzi angama-37 ° C imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.
-Isisombululo seForegene Protease Plus sinefomula ekhethekileyo, iyasebenza xa igcinwe kwindawo yokushisa ixesha elide (iinyanga ezi-3);igcinwe kwi-4 ° C, umsebenzi kunye nokuzinza kuya kuba ngcono, ngoko kuyacetyiswa ukuba uyigcine kwi-4 ° C, khumbula ukuba ungayigcini -20 ° C.
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