FFPE DNA Isolation Kit DNA Extraction Ikhithi yokuCoca kwiiSampuli zeFFPE
Inkcazelo yeKit:
Ngokukhawuleza coca i-DNA ye-genomic ekumgangatho ophezulu ukusuka kwizihlunu ezifakwe kwiparafini ezifana namacandelo eparafini kunye neebhloko zeparafini.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
Inkcazo
Le khithi inikezelwe ukukhupha kunye nokucoca i-DNA ye-genomic esemgangathweni evela kwi-formaldehyde-fixed tissue okanye i-parafini efakwe kwi-parafini.
I-DNA ye-genomic yesampula efakwe kwiparafini iya kuthotywa ngenxa yexesha lokugcina okanye indawo engqongileyo, okwenza kube nzima ukukhupha i-DNA ye-genomic.Le khithi ithatha ifomyula ekhethekileyo, efanelekile ukukhupha i-DNA ye-genomic kwiisampuli ezifakwe kwiparafini enezinga eliphezulu lempumelelo yokukhutshwa.Yonke inkqubo ayifuni ukusebenzisa i-phenol enetyhefu, i-chloroform okanye i-isopropanol precipitation.
Le khithi isebenzisa ikholomu ejikelezayo enokuthi ibophe ngokukodwa i-DNA, i-Foregene Protease Plus entsha kraca, kunye nenkqubo ye-buffer ekhethekileyo, enokuthi ikhuphe umgangatho ophezulu weparafini efakwe kwi-DNA ye-genomic kwiiyure ezingama-2-5.
Iinkcukacha
50 Preps, 100 Preps, 250 Preps
Amacandelo ekhithi
| Isithinteli FL1 |
| Isithinteli FL2* |
| Isithinteli PW* |
| Buffer WB |
| Isithinteli EB |
| Foregene Protease Plus |
| Ikholamu yeDNA-Kuphela |
| Imiyalelo |
Iimpawu&izinto eziluncedo
-Akukho ungcoliseko lwe-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
-Isantya esikhawulezayo: I-Foregene Protease Plus inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye igaya iisampulu zezicubu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwiiyure ezingama-2-5.
-Uncedo: I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4°C iqondo eliphantsi lobushushu becentrifugation okanye i-ethanol precipitation yeDNA.
-Ukhuseleko: akukho kukhutshwa kwe-organic reagent efunekayo.
-Umgangatho ophezulu: Iziqwenga ze-DNA ze-genomic ezikhutshwe zikhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
Isicelo seKit
Ifanelekile ukutsalwa kunye nokuhlanjululwa kwe-DNA ye-genomic kwiisampuli ze-formaldehyde-fixed kunye neesampuli ze-parafini ezifakwe kwi-tissue.
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
-Le khithi ingagcinwa kwiinyanga ezili-12 phantsi kweemeko ezomileyo kwindawo yokushisa (15-25 ° C);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8 ° C.
Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yitshise kwindawo yokuhlambela amanzi angama-37 ° C imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.
-Isisombululo seForegene Protease Plus sinefomula ekhethekileyo esebenzayo xa igcinwe kwindawo yokushisa ixesha elide (iinyanga ezi-3);isebenza ngakumbi kwaye izinzile xa igcinwe kwi-4 ° C, ngoko kuyacetyiswa ukuba igcinwe kwi-4 ° C, khumbula ukuba ungayigcini kwi -20 ° C.
