RT-qPCR Easyᵀᴹ (Inyathelo elinye)-Taqman
Inkcazelo yeKit:
- Nye-Ikiti yenyathelo yenza ukuba uguqulelo oluguqulelwe kunye ne-PCR luqhutywe kwityhubhu enye, kufuneka kuphela ukongeza i-template ye-RNA, iiprimers ezithile ze-PCR kunye ne-RNase-Free ddH.2O.
- Uhlalutyo lwexesha langempela lobungakanani bentsholongwane yeRNA okanye umkhondo weRNA unokuqhutywa ngokukhawuleza nangokuchanekileyo.
- Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
-Inkqubo yokusabela eyongeziweyo yenza ukusabela kube novakalelo oluphezulu lokubona, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
- RT-qPCR EasyTM(Inyathelo elinye) -Ikhithi yeTaqman iza ne-ROX yedayi yesalathiso yangaphakathi, enokusetyenziselwa ukuphelisa imvelaphi yomqondiso kunye neempazamo zesiginali phakathi kwamaqula, elungele ukusetyenziswa kwabasebenzisi bokugqibela kwiimodeli ezahlukeneyo zezixhobo zePCR zobungakanani.
Iinkcazelo
Foregene One-Inyathelo RT-PCR Easy uthotho iimveliso ziqonda ukusabela okudityanisiweyo ukusuka kwi-RNA ukuya kwi-DNA enemisonto ephindwe kabini, oko kukuthi, ukukhutshelwa umva kunye nokwandiswa kwe-PCR kugqityezelwe kwityhubhu yokusabela ye-centrifuge efanayo kunye nenkqubo yokusabela efanayo, ngaloo ndlela amanyathelo okulinga enziwe lula, isicwangciso sovavanyo siphuculwe, kunye nokuphumelela kovavanyo kuphuculwe.
RT-qPCR EasyTM (NyeInyathelo)-Taqmanusebenzisa i-Foregene HotStar Taq DNA Polymerase, inkqubo ye-Taqman qPCR ephuculweyo inokukhawuleza kwaye ngokukodwa i-RT-qPCR ye-quantitative monitoring ye-trace templates ye-RNA.
Iinkcukacha
100 Rxns (20μl inkqubo)
500 Rxns (20μl inkqubo)
Amacandelo ekhithi
| I-RT-qPCR Easy TM (Inyathelo elinye) -Taqman | ||
| Amacandelo ekhithi | RT-02131 | RT-02132 |
| 100Rxns (20μl inkqubo) | 500 Rxns (20μl inkqubo) | |
| 2× RT-qPCR EasyTMUmxube-Taqman | 1ml | 1.7ml×3 |
| I-20 × i-ROX ye-Reference Dye | 100μl | 0.5ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml×3 |
| Umyalelo wokusetyenziswa | 1 | 1 |
Iimpawu&izinto eziluncedo
-Ikiti enenyathelo elinye yenza ukuba uguqulelo oluguqulelwe kunye nePCR luqhutywe kwityhubhu enye, kufuneka kuphela ukongeza i-template ye-RNA, iiprimers ezithile zePCR kunye ne-RNase-Free ddH.2O.
- Uhlalutyo lwexesha langempela lobungakanani bentsholongwane yeRNA okanye umkhondo weRNA unokuqhutywa ngokukhawuleza nangokuchanekileyo.
- Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
-Inkqubo yokusabela eyongeziweyo yenza ukusabela kube novakalelo oluphezulu lokubona, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
- RT-qPCR EasyTM(Inyathelo elinye) -Ikhithi yeTaqman iza ne-ROX yedayi yesalathiso yangaphakathi, enokusetyenziselwa ukuphelisa imvelaphi yomqondiso kunye neempazamo zesiginali phakathi kwamaqula, elungele ukusetyenziswa kwabasebenzisi bokugqibela kwiimodeli ezahlukeneyo zezixhobo zePCR zobungakanani.
Iiparamitha zeKit
Isicelo seKit
- Le khithi isetyenziselwa ukuchongwa ngokukhawuleza nangokuchanekileyo kwe-RNA yomkhondo efana neentsholongwane ze-RNA.
- Ikulungele ngokukodwa ukufunyanwa ngokukhawuleza kweetemplates ze-RNA ezinomxholo ophezulu we-GC okanye isakhiwo sesibini esiyinkimbinkimbi.
Ukuhamba komsebenzi
Iimeko zokugcina
1.Ezothuthoiimeko
Yonke inkqubo yokuthutha i-ice box yobushushu obuphantsi, ukuqinisekisa ukuba ikiti ikwimo ye-<4 ℃.
2.Ugcino iimeko
Ikhithi igcinwe ku -20 ℃.Gcina imveliso kwifriji yokushisa rhoqo kwi-20℃ kwangoko emva kokufunyanwa.Ukuba iimeko zokugcina zifanelekile, imvelisoayisayi kuthoba umgangatho wokwenziwa komsebenzi kwisithuba sonyaka o-1 wokuqinisekisa.
