Imbewu yaseTshayina yokuTyalwa (enkulu kunye nobukhulu obuphakathi) I-PCR ethe ngqo ye-Plus Kit II-UNG(ngaphandle kweZixhobo zeSampulu) uMvelisi kunye noMboneleli |I-Foregene

Imbewu yeTyalo (enkulu kunye nobukhulu obuphakathi) I-PCR ethe ngqo kunye neKit II-UNG(ngaphandle kweZixhobo zokuSampula)

Imbewu yeTyalo (enkulu kunye nobukhulu obuphakathi) I-PCR ethe ngqo iKit II-UNG(ngaphandle kwezixhobo zeSampulu) Umfanekiso okhoyo

Inkcazelo yeKit:

Sebenzisa ngokuthe ngqo i-polysaccharide polyphenol seed lysate njenge template yokukhulisa i-PCR, ngaphandle kokutsalwa okwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.Inkqubo ye-UNG yokuchasana nongcoliseko yongezwe ukukhusela iimpawu zobuxoki.

◮Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.

◮Imfuno yesampulu incinci, thatha nje imbewu enye.

◮Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.

◮Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcazelo

Le mveliso isebenzisa inkqubo yokusabela ye-lysis ekhethekileyo, enokuthi ikhuphe ngokukhawuleza i-DNA ye-genomic kwiisampuli zembewu yezityalo kunye ne-polysaccharides ephezulu kunye ne-polyphenols (ezifana neebhanana, umqhaphu, njl.) ukuze zisetyenziswe kwiimpendulo ze-PCR, ngoko ke ifaneleka ngakumbi kuvavanyo lwemfuzo olukhulu.Ukuze kuhlangatyezwane neemfuno zeemvavanyo ezahlukeneyo zokubona, le khithi inokusebenzisa iindidi ezininzi zeesampulu (ezifana nembewu epheleleyo, ukusikwa kwezicubu ezincinci, okanye iisampuli zomhlaba zembewu ezininzi) kwiimvavanyo.Phakathi kwazo, kwiisampuli zembewu ezisafuna ukuntshula, unokukhetha ukusika iziqwenga zezicubu ezincinci (1-5mg) ngaphandle kweembumba zembewu zovavanyo;kwimifuniselo efuna iisampulu kunye novavanyo kwinani elikhulu leesampuli, ungakhetha ukuxuba iisampulu ezininzi kwaye uzidibanise.Emva kokugaya kwiinqununu ezinobubanzi obumalunga ne-0.5mm, uvavanyo lunokwenziwa (ubuncinci i-0.1% yesampuli ekujoliswe kuyo inokufunyanwa).

Iinkcukacha

50T,200T,500T,2000T

Amacandelo ekhithi

Tyala iMbewu ngqo PCR Plus Kit II-UNG Imbewu yesityalo sePolysaccharide polyphenol (enkulu naphakathi) ngqo PCR kit-UNG
Ukwakhiwa kweKit		TP-0318T	TP-03181	TP-03182	TP-03183
Ukwakhiwa kweKit		50 T	200 T	500 T	2000 T
Icandelo I	Isithinteli SSP1	24ml	96ml	120ml×2	480ml×2
	Isithinteli SP2	3ml	10ml	25ml	100ml
	I-6 × i-DNA yokulayisha i-Buffer	1.5ml	1.5ml	1.5ml	1.5ml×4
Icandelo II	Isithinteli SSP2	8ml	32ml	80ml	80ml×4
Icandelo II	2× Imbewu PCR EasyTM Mix	500μl	1ml×2	1.7ml×3	1.7ml×12
Imanyuwali		1	1	1	1

Iimpawu kunye neenzuzo

- Akukho ukuchitha ixesha kunye neendleko zokucocwa kweDNA.

- Imfuno yesampulu incinci, thatha nje imbewu enye.

- Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.

-Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors..

- Inkqubo yePCR yokuchasana nongcoliseko 2× Imbewu PCR Easy^TM Ukuxuba (UNG), enokuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR kunye nokuqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.