Blood SuperDirectᵀᴹ PCR Kit(UNG)-EDTA Blood Direct PCR Master Mix for Genotyping of Blood
Inkcazelo yeKit:
Ngokuthe ngqo sebenzisa igazi elipheleleyo le-EDTA le-anticoagulated njengethempleyithi yokukhulisa i-PCR, enovakalelo oluphezulu kunye nokusebenza kakuhle kokukhulisa.Inkqubo ye-UNG yokuchasana nongcoliseko yongezwe ukukhusela iimpawu zobuxoki.
◮Akukho mfuneko yokuhlanjululwa kwe-DNA ixesha elide kunye nexabiso eliphakamileyo, akukho nyango lwangaphambili, kunye nokusetyenziswa ngokuthe ngqo kwegazi elipheleleyo njenge-template ye-PCR.
◮Inkqubo inamandla okukhulisa amandla kwaye inokubona ngovakalelo i-genome kunye namaqhekeza e-DNA ekujoliswe kuwo angaphandle egazini.
◮2 × SuperEasyTMI-Mix (UNG) -EDTA inokunyamezela kwegazi: ukuya kwi-45% kwigazi lomntu kunye ne-20% kwigazi le-mouse.
◮Isampulu ivalwe ngokupheleleyo, ngoko ke akukho mfuneko yakuba nexhala malunga nokungcoliseka kwesampulu kunye neziphumo zobuxoki zePCR.Inkqubo yePCR echasene nongcoliseko
◮2 × SuperEasyTMI-Mix (UNG) -EDTA, iphelisa ngokufanelekileyo ukungcola okubangelwa iimveliso ze-PCR, kwaye iqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.
Iinkcukacha
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMHlanganisa (UNG) -EDTA : Kwisiseko se-2× SuperEasyTMI-Mix-EDTA, i-dUTP isetyenziswa endaweni ye-dTTP, kwaye i-enzyme ye-UNG enokuthobisa i-template equkethe i-dUTP yongezwa ngexesha elifanayo, elinokuthintela ngokufanelekileyo ukungcoliswa kweemveliso ze-PCR zokukhulisa.Iqulethe i-D-Taq DNA Polymerase elungiswe ngokukodwa, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR reaction enhancer, i-optimizer kunye ne-stabilizer, njl. njl2O ukuya kwi-2 × SuperEasy ehambelanayoTMMix(UNG)-EDTA iza kusetyenziswa kwi-PCR reaction.
I-6 × i-DNA yokuLayisha i-Buffer: I-Buffer yokuLayisha ayinayo i-SDS.Kunconywa ukusebenzisa i-6 × DNA Loading Buffer efakwe kunye nekiti xa wenza i-agarose gel electrophoresis ukuze ufumane iziphumo ezilungileyo ze-electrophoresis.Musa ukusebenzisa i-Loading Buffer equkethe i-SDS, ngaphandle koko kuya kubakho umsila omkhulu wokukhanya okukhanyayo kwindlela ngexesha le-electrophoresis, eya kuchaphazela iziphumo zovavanyo.
Amacandelo ekhithi
| 2 × SuperEasyTMUmxube(UNG)-EDTA |
| I-6 × i-DNA yokulayisha i-Buffer |
| Imiyalelo |
Iimpawu&izinto eziluncedo
- Akukho mfuneko yokuhlanjululwa kwexesha kunye neendleko ze-DNA, akukho unyango lwangaphambili, kunye nokusetyenziswa ngokuthe ngqo kwegazi elipheleleyo njenge-template ye-PCR.
-Inkqubo inamandla okukhulisa amandla kwaye inokubona ngononophelo i-genome kunye namaqhekeza e-DNA ekujoliswe kuwo angaphandle egazini.
-2 × SuperEasyTMI-Mix (UNG) -EDTA inokunyamezela kwegazi: ukuya kwi-45% kwigazi lomntu kunye ne-20% kwigazi le-mouse.
-Isampuli ivalwe ngokupheleleyo, ngoko akukho mfuneko yakukhathazeka malunga nongcoliseko lwesampulu kunye neziphumo zobuxoki ze-PCR.Inkqubo yePCR yokuchasana nongcoliseko 2× SuperEasyTMI-Mix (UNG) -EDTA, iphelisa ngokufanelekileyo ukungcola okubangelwa iimveliso ze-PCR, kwaye iqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.
Isicelo seKit
Kunikezelwe ukuchongwa ngqo kwePCR ye-EDTA anticoagulated lonke igazi.(Kubandakanya: igazi lomntu, igazi lempuku, igazi lenkukhu, igazi lentaka, igazi lenkomo, igazi lenja, njl.)
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
Le kit igcinwa kwi -20°C.
2 × SuperEasyTMMix(UNG)-EDTA，Ukuba isetyenziswa rhoqo, inokugcinwa kwi-4°C ukuze igcinwe ixesha elifutshane (isetyenziswe kwiintsuku ezili-10).
6× DNA Loading Buffer,Inokugcinwa kwi-4°C okanye -20°C ixesha elide.
