Tyala iyonke i-RNA Isolate Kit

Tyala iyonke i-RNA Isolate Kit

Inkcazo yeKiti:

Ikhithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuculwe yi-Foregene, enokukhupha ngokufanelekileyo ukusulungeka okuphezulu kunye nomgangatho opheleleyo we-RNA kwizicubu zezityalo ezahlukeneyo ezineepolysaccharides ezisezantsi kunye nomxholo we polyphenols. Kwiisampulu zezityalo ezine-polysaccharides ephezulu okanye umxholo we-polyphenols, kuyacetyiswa ukuba kusetyenziswe i-Plant Total RNA Isolate Plus Kit ukufumana iziphumo ezingcono zeRNA. Ikhithi ibonelela ngekholamu yokucoca i-DNA enokususa ngokulula i-genomic DNA kwi-supernatant kunye ne-lysate yethishu. Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA. Ikhithi inokuqhubekeka ngenani elikhulu leesampulu ngaxeshanye.

Inkqubo yonke ayiqulathanga i-RNase, ke i-RNA ecociweyo ayizukuthotywa. I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotein, iDNA, ion, kunye nezinto eziphilayo.


Iinkcukacha Product

Iimpawu zemveliso

Ukucaciswa

50 Preps, 200 Preps

Izixhobo zekiti

IBuffer PSL1, iBuffer PS, iBuffer PSL2

IBuffer PRW1, iBuffer PRW2

RNase-Simahla ddH2O, Ikholamu yokucoca i-DNA

RNA-Ikholamu kuphela

Imiyalelo

Iimpawu kunye nezibonelelo

■ Ukusebenza kubushushu begumbi (15-25 ℃) kuyo yonke inkqubo, ngaphandle kwebhafu yomkhenkce kunye neqondo lobushushu eliphantsi.
■ Gcwalisa ikhithi ye-RNase-Free, akukho sidingo sokukhathazeka malunga nokuthotywa kweRNA.
■ Ikholamu yokucoca i-DNA ibophelela ngqo kwi-DNA, ukuze ikiti isuse ungcoliseko lwe-genomic ye-DNA ngaphandle kokongeza i-DNase.
■ Ukuveliswa okuphezulu kweRNA: Ikholamu ye-RNA kuphela kunye nefomula eyahlukileyo inokuyicoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwisithuba semizuzu engama-30.
■ Ukhuseleko: akukho reagent yendalo iyafuneka.
■ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo asulungeke kakhulu, awanayo iprotheyini kunye nokunye ukungcola, kwaye anokuhlangabezana nezicelo ezahlukeneyo zovavanyo.

Isicelo seKiti

Kukulungele ukukhutshwa nokucocwa kwe-RNA iyonke kwiisampulu zezityalo ezisandul 'ukutsha okanye ezinqabileyo (ngakumbi izicwili zamagqabi ezityalo) kunye nomxholo we-polysaccharide kunye nomxholo we-polyphenol.

Ukuhamba komsebenzi

plant total RNA-simple workflow

Umzobo

Plant Total RNA Isolation Kit6

I-Plant Total RNA Isolate Kit Plus iqhutywe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kwaye i-5% ye-RNA ehlambulukileyo yavavanywa yi-electrophoresis.
1: Ibhanana
2: IGinkgo
3: Umqhaphu
4: iRharnate

Ukugcinwa kunye neshelf life

Ikhithi ingagcinwa kangangeenyanga ezili-12 kubushushu begumbi (15-25 ℃) kwindawo eyomileyo, kunye ne-2-8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer PSL1 inokugcinwa kwi-4 ℃ kangangenyanga enye emva kokudibanisa i-2-hydroxy-1-ethanethiol (ngokuzithandela).


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  • Okulandelayo:

  • Bhala umyalezo wakho apha uze uyithumele kuthi

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