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QuickEasyᵀᴹ Ixesha lokwenyani PCR Kit-Taqman

QuickEasyᵀᴹ Ixesha lokwenyani PCR Kit-Taqman Featured Image
  • QuickEasyᵀᴹ Ixesha lokwenyani PCR Kit-Taqman

Inkcazelo yeKit:

◮Inkqubo ekhethekileyo yokuphucula iPCR yenza i-2×QuickEasyTMReal PCR Mix-Taqman ehambelana ngakumbi.

◮Ukuqala okushushu kwe-Foregene HS Taq Polymerase inomgangatho ophezulu wokukhulisa umvandedwa, uvakalelo oluphezulu lokukhulisa, kunye nokucaciswa okuphezulu kokukhulisa.

◮2× QuickEasyTMI-Real PCR Mix -i-Taqman isebenzisa inkqubo ekhethekileyo eyenziwe yi-Foregene ukuphucula ubuntununtunu kunye neenkcukacha zokufumanisa ulandelelwano oluthile lwe-probe, enokusetyenziswa kwi-genotyping kunye nokukopisha ukuhluka kwenombolo kunye nokufumana iziphumo ezichanekileyo.

◮Le mveliso iza nedayi yesalathiso yangaphakathi ye-ROX, enokusetyenziselwa ukuphelisa imvelaphi yomqondiso kunye nempazamo yomqondiso phakathi kwamaqula, ekulungeleyo ukuba abathengi basebenzise kwiintlobo ezahlukeneyo zezixhobo zePCR zobungakanani.

amandla angaphambili


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FAQ

Iinkcazelo

I-2 × QuickEasyTMYenyani PCR Mix-Taqman ibonelelwe yi QuickEasyTMIxesha lokwenyani lePCR Kit-Taqman kit sisizukulwana esitsha sokuxuba inkqubo eyilelwe ukusabela kokukhulisa i-PCR ngexesha lokwenyani kusetyenziswa iiprobe ezithile zefluorescent.Undoqo wayo we-Foregene HS Taq DNA Polymerase usekwe kuhlengahlengiso lwe-Antibody, esetyenziswa ngokubambisana ne-Foregene eyongezelelekileyo inkqubo yePCR, inokucaciswa okuphezulu kunye nempumelelo yokukhulisa.Xa kuthelekiswa nomxube oqhelekileyo wePCR, inamandla okukhulisa amandla kunye nesantya esisezantsi sokungafani.Ingasetyenziswa kwi-fluorescence quantitative PCR reaction ukunciphisa ukukhulisa okungangqalanga kunye nokuphucula ukuchaneka kwe-PCR.Inokuphucula kakhulu imveliso ethile kunye novakalelo lokusabela.Kwangelo xesha, i-ROX yanikezelwa njengedayi yereferensi yangaphakathi.

I QuickEasyTMIxesha langempela le-PCR Kit-Taqman kit inobubanzi obubanzi besicelo kwisixa setemplate, kwaye inokufumana igophe eliqhelekileyo elisemgangathweni kwimimandla eyahlukeneyo yobuninzi, kwaye inokulinganisa ngokuchanekileyo kunye nokufumanisa ijene ekujoliswe kuyo ngokuphindaphinda okulungileyo kunye nokuzithemba okuphezulu.

Iinkcukacha

Amalungiselelo angama-200,500 Preps,1000 Preps,2000 Preps

Amacandelo ekhithi

QuickEasyTMIxesha lokwenyani PCR Kit-Taqman(20μlinkqubo)

Imixholo yekhithi

QP-01121

QP-01122

QP-01123

QP-01124

200 Preps

500 Preps

1000 Preps

2000 Preps

2×NgokukhawulezaKululaTM I-PCR yokwenyani Xuba-Taqman

1 ml×2

1.7 ml×3

1.7 ml×6

1.7 ml×12

20×I-ROX Reference Daye

200μL

0.5 ml

1 ml

1 ml×2

DNase-Free ddH2O

1.7 ml

1.7 ml×2

10 ml

20 ml

IFU

1iqhekeza

1iqhekeza

1iqhekeza

1iqhekeza

Iimpawu&izinto eziluncedo

■ Inkqubo eyodwa yokuphucula iPCR yenza i-2×QuickEasyTMReal PCR Mix-Taqman ehambelana ngakumbi.

■ I-Hot-start I-Foregene HS Taq Polymerase inokwandiswa komgangatho ophezulu, uvakalelo oluphezulu lwe-amplification, kunye neenkcukacha eziphakamileyo zokukhulisa.

■ 2× QuickEasyTM I-Real PCR Mix -i-Taqman isebenzisa inkqubo ekhethekileyo eyenziwe yi-Foregene ukuphucula ubuntununtunu kunye neenkcukacha zokufumanisa ulandelelwano oluthile lwe-probe, enokusetyenziswa kwi-genotyping kunye nokukopisha ukuhluka kwenombolo kunye nokufumana iziphumo ezichanekileyo.

■ Le mveliso iza nedayi yesalathiso yangaphakathi ye-ROX, enokusetyenziswa ukuphelisa imvelaphi yomqondiso kunye nempazamo yomqondiso phakathi kwamaqula, ekulungeleyo ukuba abathengi basebenzise kwiintlobo ezahlukeneyo zezixhobo ze-PCR zobungakanani.

Isicelo seKit

■ Fluorescence quantitative PCR yohlalutyo lobungakanani betemplate;

■ Culwandiso lwePCR olwenziwayo;

■ Ingasetyenziselwa ukufumanisa i-allele.

Ugcino kunye nobomi beShelf

Ikiti kufuneka igcinwe kwi -20 ° C ebumnyameni;ukuba isetyenziswe rhoqo, ingaphinda igcinwe kwi-4 ° C ixesha elifutshane (sebenzisa kwiintsuku ezili-10).

  • Ngaphambili:
  • Okulandelayo:

    • Ixesha lokwenyani imigaqo yoyilo lwe-PCR primer

    I-Primer yokuPhambili kunye ne-Primer yokuBuyisa

    Ngexesha lokwenyani iPCR, uyilo lweprimer lubaluleke kakhulu.Iiprimers zinxulumene nobucacileyo kunye nokusebenza kakuhle kokwandiswa kwePCR, kwaye zinokuyilwa ngokubhekiselele kule migaqo ilandelayo:

    Ubude bePrimer: 18-30bp.

    Umxholo weGC: 40-60%.

    Ixabiso le-Tm: I-software yoyilo lwe-Primer, njenge-Primer 5, inokunika ixabiso le-Tm le-primer.Amaxabiso e-Tm ee-primers ezinyukayo nezisezantsi kufuneka zibe kufutshane kangangoko.Ifomula yokubala ye-Tm nayo ingasetyenziswa: Tm = 4 °C (G + C) + 2 °C (A + T).Xa kusenziwa i-PCR, iqondo lobushushu elingaphantsi kwexabiso le-primer Tm le-5 °C likhethwa ngokubanzi njengeqondo lobushushu lokutshisa (ukunyuka okuhambelanayo kwiqondo lobushushu lobushushu kunokunyusa ukuchana kwe-PCR reaction).

    Iiprimers kunye neemveliso zePCR:

    I-design primer PCR yokukhulisa ubude bemveliso ikhethwa yi-100-150bp.

    Ii-primers zoyilo kwindawo yesibini yesakhiwo setemplate kufuneka igwenywe kangangoko kunokwenzeka.

    Gwema ukubunjwa kweziseko ezi-2 okanye ngaphezulu ezihambelanayo phakathi kwee-3′ zeziphelo ze-primers ezinyukayo kunye nezantsi.

    Isiseko se-Primer 3′ asikwazi ukubakho nge-3 eyongezelelweyo elandelelanayo i-G okanye i-C.

    Iiprimers ngokwazo azikwazi ukuba nezakhiwo ezincedisayo, ngaphandle koko kuya kwenziwa isakhiwo se-hairpin, esichaphazela ukukhulisa i-PCR.

    I-ATCG kufuneka isasazwe ngokulinganayo ngokulandelelana kwe-primer, kwaye isiseko se-terminal ye-3 kufuneka sigwenywe njenge-T.

    ISihlomelo 1: Ipakethi yecandelo leCell Direct RT-qPCR Kit

    1.Iseli Lysis Solution


    Iseli Lysis Solution

    Amacandelo ekhithi

    (Inkqubo ye-lysis ye-24 / kakuhle)

    DRT-01011-A1

    DRT-01011-A2

    100 T

    500 T

    InxalenyeI

    Isithinteli CL

    20 ml

    100 ml

    Foregene Protease Plus II

    400 μl

    1 ml × 2

    Isithinteli ST

    1 ml × 2

    10 ml

    InxalenyeII

    DNA Eraser

    400 μl

    1 ml × 2

    2.RT Umxube


    RT Umxube

    Amacandelo ekhithi

    (20 μl inkqubo yokusabela)

    DRT-01011-B1

    200 T

    5 × Ngqo RT Mix

    800 μl

    RNase-Free ddH2O

    1.7 ml × 2

     

    3.qPCR Mix


    Umxube we-qPCR

    Amacandelo ekhithi

    (20 μl inkqubo yokusabela)

    DRT-01011-C1

    DRT-01011-C2

    200 T

    1000 T

    2 × Ngqo qPCR Mix-SYBR

    1 ml × 2

    1.7 ml × 6

    I-50 × i-ROX Reference Dye

    40 μl

    200 μl

    RNase-Free ddH2O

    1.7 ml

    10 ml
    Bhala umyalezo wakho apha kwaye uwuthumele kuthi

    EzinxulumeneyoIimveliso

    Cofa u-enter ukukhangela okanye i-ESC ukuvala