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I-Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)

I-Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish) Umfanekiso okhoyo
  • I-Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)
  • I-Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)
  • I-Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)
  • I-Zebra Fish Direct PCR Kit-UNG Direct PCR Lysis Reagent(zebrafish)

Inkcazelo yeKit:

Ukusetyenziswa ngokuthe ngqo kwe-zebrafish lysate njenge template yokukhulisa i-PCR, akukho kukhutshwa kwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.Kongezwe inkqubo ye-UNG yokuchasana nokungcoliseka kongcoliseko ukuze kupheliswe iimpawu zobuxoki.

◮Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.

◮Imfuno yesampulu incinci, thatha nje imbewu enye.

◮Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.

◮Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.

amandla angaphambili


Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcazelo

Le khithi isebenzisa inkqubo ye-lysis buffer ekhethekileyo ukukhulula ngokukhawuleza i-DNA ye-genomic kwi-zebrafish kunye nezinye izihlunu zentlanzi zamanzi ahlambulukileyo, ama-fins omsila okanye iisampuli zeqanda leentlanzi kwiimpendulo ze-PCR, ngoko ke ifaneleka ngakumbi kuvavanyo lwemfuzo olukhulu.Inkqubo yokukhulula i-DNA ye-genomic kwi-lysis buffer igqityiwe ngaphakathi kwe-10-30 imizuzu kwi-65 ° C.Azikho ezinye iinkqubo ezifana neprotheyini kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA ungasetyenziswa njenge template ye-PCR reaction.

I-2 × PCR Easy TM Mix (UNG) isebenzisa i-dUTP endaweni ye-dTTP ngesiseko se-2 × PCR Easy TM Mix, kwaye yongeza i-UNG enzyme (i-Uracil-N-glycosylase) enokunciphisa i-template equkethe i-dUTP ngexesha elifanayo.Ngaphambi kokuphendula kwe-PCR, i-enzyme ye-UNG isetyenziselwa ukuthotywa kwemveliso ye-PCR equkethe i-uracil.I-enzyme ye-UNG ayiyi kuba nayiphi na impembelelo kwi-template engenawo i-uracil, ngaloo ndlela iqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa kunye nokuthintela ukuvavanywa kofuzo olukhulu.Usulelo lweemveliso zePCR lwenzekile.

I-D-Taq DNA polymerase yipolymerase ye-DNA ephuhliswe ngokukodwa yi-Foregene ukwenzela ukuphendula ngokuthe ngqo kwe-PCR.I-D-Taq DNA polymerase inonyamezelo oluqinileyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokwandisa ngokufanelekileyo izixa ze-DNA kwiinkqubo ezahlukeneyo zokusabela ezintsonkothileyo, kunye nesantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo kakhulu kwi-Direct PCR reaction.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Icandelo I

Buffer FP

I-Foregene Protease

I-6 × i-DNA yokulayisha i-Buffer

Icandelo II

2×PCR EasyTMUmxube(UNG)

Imiyalelo

Iimpawu&izinto eziluncedo

∎ Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha nokubiza imali eninzi.

∎ Imfuno yesampulu incinci, kancinane njenge-1mg ye-caudal fin yentlanzi okanye amaqanda entlanzi ali-10 anokuhlolwa.

∎ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.

■ Inkqubo ye-PCR ye-Anti-pollution 2 × PCR Easy TM Mix (UNG), enokuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR kunye nokuqinisekisa ukuchaneka kunye nokuchaneka kokukhulisa.

■ Inkqubo ye-PCR ephuculweyo yenza ukuba i-PCR ibe nezinto ezicacileyo eziphezulu kunye nokunyamezelana okunamandla kwii-PCR reaction inhibitors.

Iiparamitha zeKit

Isicelo: Iintlanzi zeZebra kunye nezinye iintlanzi zamanzi ahlambulukileyo.

I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.

Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.

Ukuhamba komsebenzi

intlanzi zebra ngqo PCR

Ugcino kunye nobomi beShelf

Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.

I-reagent Buffer FP inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃ ukuze igcinwe ixesha elide.

v IReagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine kwi-4 ℃ iinyanga ezili-12.

vReagent 6×DNA Loading Buffer ingagcinwa kwi 4℃ okanye -20℃ ixesha elide.

Icandelo II lale khithi kufuneka ligcinwe kwi -20 ℃.

I-vReagent 2 × PCR Easy TM Mix (UNG), ukuba isetyenziswe rhoqo, ingaphinda igcinwe kwi-4 ℃ yokugcina ixesha elifutshane (ukusebenzisa kwiintsuku ze-10).

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