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Tyala iMbewu ngokuNgqo kwiKhithi ye-PCR I/II (ngaphandle kweZixhobo zokuSampula) Iikiti ze-PCR zeProtocol

Tyala iMbewu ngokuthe ngqo kwiKhithi ye-PCR ye-I/II (ngaphandle kweZixhobo zeSampulu) iProtocol Plant Direct Direct PCR Kits Featured Image
  • Tyala iMbewu ngokuNgqo kwiKhithi ye-PCR I/II (ngaphandle kweZixhobo zokuSampula) Iikiti ze-PCR zeProtocol
  • Tyala iMbewu ngokuNgqo kwiKhithi ye-PCR I/II (ngaphandle kweZixhobo zokuSampula) Iikiti ze-PCR zeProtocol
  • Tyala iMbewu ngokuNgqo kwiKhithi ye-PCR I/II (ngaphandle kweZixhobo zokuSampula) Iikiti ze-PCR zeProtocol
  • Tyala iMbewu ngokuNgqo kwiKhithi ye-PCR I/II (ngaphandle kweZixhobo zokuSampula) Iikiti ze-PCR zeProtocol

Inkcazelo yeKit:

◮Akukho xesha lidla kwaye libiza kakhulu ukucocwa kweDNA (Ixesha, umsebenzi kunye neendleko zigcinwe!)

◮Izinto ezincinci

◮Elula-isampulu inokuthi ixhomekeke kwi-PCR reaction okanye i-lysis reaction ngaphandle kokusika okanye ukugaya

◮I-2 X Mix-ukunciphisa iimpazamo zokulayisha isampuli kunye nexesha lokulungiselela inkqubo yokusabela

◮Ukulungiswa ngokukhawuleza kwe-template kunokugqitywa kwimizuzu eyi-10, ukuphendula kwe-PCR kunokugqitywa kwimizuzu engama-50

◮I-high throughput-lysis reaction ingagqitywa kwi-96-well PCR plate

amandla angaphambili


Khuphela njengePDF

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcazelo

Le mveliso isebenzisa inkqubo eyodwa ye-lysis buffer ukuze i-lyse imbewu yezityalo.I-lysate ingasetyenziselwa njenge template ngaphandle kokuhlanjululwa.Emva kokongeza i-primers, i-PCR Mix yale khithi ingasetyenziselwa ukukhulisa.

Iinkcukacha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Amacandelo ekhithi

Tyala iMbewu ngokuNgqo PCR Kit I

Icandelo I

Isithinteli SP1

Isithinteli SP2

I-6×I-DNA yokuLayisha i-Buffer

Icandelo II

I-2 × Imbewu yePCR ilulaTMXuba

Tyala iMbewu eNgqo kwiPCR Kit II

Icandelo I

Isithinteli SP1

Isithinteli SP2

I-6×I-DNA yokuLayisha i-Buffer

Icandelo II

I-2 × Imbewu yePCR ilulaTMXuba

Iimpawu&izinto eziluncedo

■ Akukho kutya ixesha kunye nokucoca i-DNA ebiza imali eninzi

■ imathiriyeli encinci

■ Ilula-isampuli inokuxhomekeka kwi-PCR reaction okanye i-lysis reaction ngaphandle kokusika okanye ukugaya

■ 2X Mix-ukunciphisa iimpazamo zokulayisha isampuli kunye nexesha lokulungiselela inkqubo yokusabela

■ Ukukhawuleza-ukulungiswa kwetemplate kunokugqitywa kwimizuzu eyi-10, ukuphendula kwe-PCR kungagqitywa kwimizuzu engama-50

■ Ukusebenza okuphezulu-reaction lysis inokugqityezelwa 96-kakuhle PCR ipleyiti.

Iiparamitha zeKit

Isicelo: Ukuchongwa kwezityalo ze-transgenic, i-genotyping, njl.

Isampuli: Imbewu yokutyala okanye izicubu ezine-polysaccharide ephantsi kunye nomxholo we-polyphenol.

Ubungakanani besampuli: Imbewu enye yonke, i-1-100mg yezityalo zesityalo

Uluhlu lokufumanisa: imveliso yePCR ≤1kb

Ukuhamba komsebenzi

tyala imbewu ngqo PCR

Electropherogram

tyala imbewu ngqo PCR-DIAGRAM
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