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I-Plat DNA Isolation Kit Genomic Plant DNA yokuCoca iiKits Protocol
Ikati.No.DE-06111/06112/06113
Ukuhlanjululwa kwe-DNA ye-genomic kwiithishu zezityalo ezahlukeneyo.
Ngokukhawuleza uhlambulule kwaye ufumane i-DNA ye-genomic ephezulu kwiisampuli zezityalo (kubandakanya i-polysaccharides kunye neesampuli zezityalo ze-polyphenol).
◮Akukho usulelo lwe-RNase
◮Isantya esikhawulezayo
◮Elula: Umsebenzi wokucoca unokugqitywa kwimizuzu engama-30.
◮Iluncedo: Ubushushu begumbi, i-4℃ centrifugation kunye ne-ethanol precipitation ye-DNA ayifuni.
◮Ukhuseleko: akukho reagent ephilayo esetyenziswayo.
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I-Animal Tissue DNA Isolation Kit DNA Extraction Kits from Animal Tissue Samples
Ukukhutshwa ngokukhawuleza kunye nokucocwa kwe-DNA ye-genomic kwimithombo emininzi, njengezicubu zezilwanyana, iiseli, njl.
◮Isantya esiphezulu:umsebenzi ophezulu kunama-proteases afanayo kunye nokugaya iisampuli zezicubu ngokukhawuleza;
◮Lula:inokugqitywa kwimizuzu engama-50.
◮Iluncedo:Yenziwe kwiqondo lokushisa.
◮Ukhuseleko:akukho reagent ephilayo esetyenziswayo.
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Blood DNA Mini Kit
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu kwigazi eli-anticoagulated (<1ml).
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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Ngokubanzi iPlasmid Mini Kit General Plasmid DNA Extraction Miniprep Mini Kits
Ucoca ngokukhawuleza i-plasmid DNA ekumgangatho ophezulu ukusuka kwiibhaktheriya eziguquliweyo zovavanyo lwesiqhelo lwebhayoloji yemolekyuli njengotshintsho kunye nokwetyisa kwe-enzyme.
◮Susa i-RNA ngaphandle kokongeza i-RNAse
◮Iluncedo-i-centrifugation yenziwa kwindawo yobushushu begumbi
◮Ukukhawuleza-umsebenzi unokugqitywa kwimizuzu engama-20
◮Ikhuselekile-akukho reagent ephilayo esetyenzisiweyo
◮Ukucoceka okuphezulu—OD260/280≈1.7-1.9
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I-Blood DNA Midi Kit (1-5ml) I-Blood Isolation Midi Kits ye-DNA kwiGazi
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu kwigazi eli-anticoagulated (1-5ml).
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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Buccal Swab/FTA Card DNA Isolation Kit Genomic DNA Extraction okanye Purifiction Kit from Buccal Swabs
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu kwi-buccal swab / iisampulu zeKhadi le-FTA.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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Genomic DNA Micro Kit Micro Genomic DNA Mini Kit
Ngokukhawuleza uhlambulule iisampuli ezincinci ukufumana i-DNA ye-genomic ephezulu.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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Ikhithi yokuzahlula ye-DNA engenaSeli
Ukwahlukaniswa kwe-cfDNA kwiisampulu zeplasmaInombolo yekhathalogu TQ01BT0050, TQ01BT0100 -
Amanzi I-DNA Isolation Kit DNA Extraction and Purification Kit for Water
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu evela kwi-microorganisms kwiisampuli zamanzi ezahlukeneyo.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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I-Stool DNA Isolation Kit DNA Extraction Iikhithi zokucoca ukusuka kwisitulo
Ucoca ngokukhawuleza kwaye ufumane i-DNA ye-genomic ekumgangatho ophezulu kwiisampulu zesitulo ezahlukahlukeneyo.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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I-PCR yokucoca iKhiti ye-PCR yokuCoca-up System ye-Ultra-pure DNA
Ngokukhawuleza uhlambulule kwaye ufumane amaqhekeza e-DNA akumgangatho ophezulu kwinkqubo yePCR;ukuba ufuna ukwahlula kwaye ufumane amaqhekeza athile eDNA, nceda ukhethe ikhithi yokubuyisela ijeli.
◮ Uluhlu olubanzi lokuchacha kwe-DNA: Iziqwenga ze-DNA zifutshane njenge-60bp kwaye ezinkulu njenge-10kb zinokufunyanwa.
◮ Ukusebenza okuphezulu kokubuyisela: Ukusebenza kokubuyisela ngokuqhelekileyo kungaphezulu kwe-80%, kwaye iphakamileyo inokufikelela ngaphezu kwe-95%.
◮ Isantya esiphezulu:Kulula ukuyisebenzisa, ukubuyiswa kweqhekeza le-DNA kunokugqitywa kwimizuzu eyi-15 kwaye i-primer dimer inokususwa ngokuthe ngqo ngaphandle kokubuyiswa kwe-gel.
◮ Safety:Akukho kutsalwa kwe-organic reagent efunekayo.
◮ Umgangatho ophezulu: Amaqhekeza e-DNA afunyenweyo acoceke kakhulu, anokudibana novavanyo olwahlukeneyo olulandelayo.
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FFPE DNA Isolation Kit DNA Extraction Ikhithi yokuCoca kwiiSampuli zeFFPE
Ngokukhawuleza coca i-DNA ye-genomic ekumgangatho ophezulu ukusuka kwizihlunu ezifakwe kwiparafini ezifana namacandelo eparafini kunye neebhloko zeparafini.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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