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I-PCR yokucoca iKhiti ye-PCR yokuCoca-up System ye-Ultra-pure DNA
Ngokukhawuleza uhlambulule kwaye ufumane amaqhekeza e-DNA akumgangatho ophezulu kwinkqubo yePCR;ukuba ufuna ukwahlula kwaye ufumane amaqhekeza athile eDNA, nceda ukhethe ikhithi yokubuyisela ijeli.
◮ Uluhlu olubanzi lokuchacha kwe-DNA: Iziqwenga ze-DNA zifutshane njenge-60bp kwaye ezinkulu njenge-10kb zinokufunyanwa.
◮ Ukusebenza okuphezulu kokubuyisela: Ukusebenza kokubuyisela ngokuqhelekileyo kungaphezulu kwe-80%, kwaye iphakamileyo inokufikelela ngaphezu kwe-95%.
◮ Isantya esiphezulu:Kulula ukuyisebenzisa, ukubuyiswa kweqhekeza le-DNA kunokugqitywa kwimizuzu eyi-15 kwaye i-primer dimer inokususwa ngokuthe ngqo ngaphandle kokubuyiswa kwe-gel.
◮ Safety:Akukho kutsalwa kwe-organic reagent efunekayo.
◮ Umgangatho ophezulu: Amaqhekeza e-DNA afunyenweyo acoceke kakhulu, anokudibana novavanyo olwahlukeneyo olulandelayo.
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I-Bacterial DNA Isolation Kit I-Bacterial Genomic DNA Extraction Iikhithi zokuCoca
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu evela kwiibhaktheriya kwisigaba sokukhula kwe-logarithmic.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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Mouse Umsila DNA Mini Kit Genomic DNA Extraction Kits ukusuka Mouse Umsila
Eyona khithi ikhawulezayo yehlabathi yokukhupha umsila wemouse i-genomic DNA, enokukhupha i-DNA ye-genomic ekumgangatho ophezulu kumsila wempuku kwiyure enye.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
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Gel Extraction Kit DNA Gel Extraction Kit
Ngokukhawuleza nangempumelelo buyisa amaqhekeza e-DNA angama-20bp-10kb kwijeli yeagarose.
◮Uluhlu olubanzi lokubuyiselwa kweDNA:Iziqwenga ze-DNA ezimfutshane njenge-30bp kwaye ezinkulu njenge-10kb zinokufunyanwa.
◮Ukusebenza okuphezulu kokubuyisela:Ukusebenza okuphezulu kokubuyisela kunokufikelela ngaphezu kwe-80%.
◮Inkqubo encinci elution:ubuncinane 30μl elution isisombululo ingasetyenziselwa elution, nto leyo enokuthi ngempumelelo ukunyusa uxinaniso amaqhekeza wachacha DNA.
◮Isantya esiphezulu:Kulula ukuyisebenzisa, ukubuyiswa kweqhekeza le-DNA kunokugqitywa kwimizuzu eyi-15.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:Amaqhekeza e-DNA afunyenweyo acoceke kakhulu, anokudibana nemifuniselo eyahlukeneyo elandelayo.
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EndoFree Maxi Plasmid Kit (Spin Column)
Yonke inkqubo yokutsalwa ithatha iyure enye kuphela, iqinisekisa ukusebenza ngokufanelekileyo nangokukhawulezayo.
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