Blood DNA Mini Kit
Inkcazelo yeKit:
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu kwigazi eli-anticoagulated (<1ml).
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
Iinkcazelo Kit
Le khithi ithatha i-Foregene Protease Plus entsha kunye ne-BL1 kunye ne-BL2 ye-buffer systems, ekwazi ukwetyisa ngokupheleleyo iisampuli zegazi ze-anticoagulant ngexesha elifutshane, ngaloo ndlela inqanda ukuthotywa kwe-DNA ukuya kwinqanaba eliphezulu kunye nokufumana ubuninzi be-DNA ye-genomic.Inkqubo yokucubungula igazi ngokukhawuleza kunye ne-DNA-Only column operation lula kakhulu ukutsalwa kwe-genome yegazi, okwenza i-DNA ye-genomic inokufumaneka kumgangatho ophezulu kunye nokucoceka okuphezulu kwimizuzu engama-40.
I-DNA-kuphela kwe-silica gel membrane esetyenziswe kwikholamu ye-centrifuge yale kit luhlobo olutsha lwezinto ezibonakalayo kwinkampani yethu, enokuthi ibophe i-DNA ngokufanelekileyo kwaye ngokuthe ngqo, kwaye inokususa i-RNA, ukungcola, iiprotheni, i-ion kunye nezinye izinto eziphilayo kwiiseli ukuya kwinqanaba eliphezulu.Iziqwenga ze-DNA ezifunyenweyo zikhulu, zicocekile, zizinzile kwaye zithembekile umgangatho.Ubuninzi bokuthwala umthamo we-DNA-Only column yi-80 μg DNA.I-DNA efunyenweyo ingasetyenziselwa kwiimvavanyo zebhayoloji ye-molekyuli ezifana ne-enzyme digestion, i-PCR, i-hybridization yaseMzantsi kunye nokwakhiwa kwethala leencwadi.
Ikiti inokuqhubela phambili ukuya kwi-1ml yegazi ngexesha lokufumana inani elaneleyo le-DNA ye-genomic ephezulu.
Iinkcukacha
| Blood DNA Mini Kit | |||
| Imixholo yekhithi | DE-05111 | DE-05112 | DE-05113 |
| 50 T | 100 T | 250 T | |
| Isithinteli BL1 | 15 ml | 30 ml | 75 ml |
| Isithinteli BL2 * | 15 ml | 30 ml | 75 ml |
| Buffer DC | 100 ml | 200 ml | 500 ml |
| Isithinteli PW * | 25 ml | 50 ml | 125 ml |
| Isithinteli WB1 | 15 ml | 30 ml | 75 ml |
| Isithinteli EB | 10 ml | 20 ml | 50 ml |
| Foregene Protease Plus | 1.25 mL | 2.5 ml | 6.5 ml |
| Ikholamu yeDNA-Kuphela | 50 | 100 | 250 |
| Imanyuwali | 1 | 1 | 1 |
*: I-Buffer BL2 kunye ne-Buffer PW ziqulathe ityuwa elulwelo olucaphukisayo.Nceda unxibe iiglavu kwaye uthathe amanyathelo okhuseleko afanelekileyo xa usebenza.
Iimpawu&izinto eziluncedo
-Akukho ungcoliseko lwe-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
-Isantya esikhawulezayo: I-Foregene Protease Plus inomsebenzi ophezulu kune-proteases efanayo, kwaye igaya iisampulu zezicubu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-40.
-Uncedo: I-centrifugation yenziwa kwiqondo lokushisa, i-4 ° C i-centrifugation okanye i-ethanol precipitation ye-DNA ayifuni.
-Ukhuseleko: Akukho kukhutshwa kwezinto eziphilayo ezisetyenzisiweyo.
-Umgangatho ophezulu: Iziqwenga ze-DNA ze-genomic ezikhutshwe zikhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
Isicelo seKit
Ikulungele ukutsalwa kunye nokucocwa kwe-DNA ye-genomic kwigazi elitsha okanye elinomkhenkce elichasene ne-anticoagulated.
Ukuhamba komsebenzi
Ukugcinwa kunye nobomi beshelufu
Le khithi ingagcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo kwiqondo lokushisa (15-25 ° C);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8 ° C.
Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yitshise kwindawo yokuhlambela amanzi angama-37 ° C imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.
Isisombululo se-Foregene Protease Plus sinefomula ekhethekileyo, esebenzayo xa igcinwe kwindawo yokushisa ixesha elide (iinyanga ezi-3);iya kusebenza ngakumbi kwaye izinzile xa igcinwe kwi-4 ° C, ngoko kuyacetyiswa ukuba igcinwe kwi-4 ° C, khumbula ukuba ungayigcini -20 ° C.
