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Ixesha lokwenyani PCR Easyᵀᴹ-SYBR Green I kit
Lula—2X PCR Mix ukunciphisa impazamo yovavanyo kunye nexesha lokusebenza
I-Specific-optimized buffer kunye ne-Hot-start Taq enzyme inokuthintela ukukhulisa okungangqalanga kunye nokwakheka kwe-primer dimer.
Uvakalelo oluphezulu-luyakwazi ukubona iikopi eziphantsi zetemplate
Ukuguquguquka okuhle-kuyahambelana nezixhobo ezininzi zePCR zexesha lokwenyani
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I-RT-PCR Easyᵀᴹ I(Inyathelo elinye)
◮Ikiti yenyathelo elinye yenza ukuba uguqulelo loguqulelo kunye nePCR luqhutywe kwityhubhu enye.Ifuna kuphela ukongeza ithemplate ye-RNA, iiprimers ezithile zePCR kunye ne-RNase-Free ddH2O.
◮Uhlalutyo lwexesha langempela lobungakanani be-RNA lunokwenziwa ngokukhawuleza nangokuchanekileyo.
◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
◮Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
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I-RT-PCR Easyᵀᴹ II(Amanyathelo amabini)
◮Uhlalutyo lwexesha langempela lobungakanani be-RNA lunokwenziwa ngokukhawuleza nangokuchanekileyo.
◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
◮ Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
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Ixesha lokwenyani PCR Easyᵀᴹ-Taqman
◮Elula—2× PCR Mix ukunciphisa impazamo yovavanyo kunye nexesha lokusebenza
◮I-Specific-optimized buffer kunye ne-Hot-start Taq enzyme inokuthintela ukukhulisa okungangqalanga kunye nokwakheka kwe-primer dimer.
◮Uvakalelo oluphezulu-luyakwazi ukubona iikopi eziphantsi zetemplate
◮Ukuguquguquka okuhle-kuyahambelana nezixhobo ezininzi zePCR zexesha lokwenyani
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PCR Heroᵀᴹ (Ngedayi)
◮Ukunyaniseka okuphezulu: amaxesha ama-6 e-enzyme yeTaq eqhelekileyo;
◮Isantya sokukhulisa ngokukhawuleza
◮Ukuziqhelanisa ngakumbi kwetemplate
◮Ukusebenza kokwandisa okuphezulu
◮Ukunyamezela kokusingqongileyo kunamandla: kubekwe kwi-37 ° C ngeveki, ukugcina umsebenzi ongaphezu kwe-90%;
◮Ino-5'→3' DNA polymerase umsebenzi kunye 5'→3' exonuclease umsebenzi, ngaphandle 3'→5' umsebenzi exonuclease.
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700bp DNA Ileli
◮I-700bp DNA Ladder yimveliso ekulungele ukusetyenziswa kwaye sele iqulethe i-1x yokulayisha i-Buffer.Unokuthatha ngokuthe ngqo inani elifanelekileyo leMarker ye-electrophoresis ngokweemfuno zovavanyo.
700bpI-DNA Marker Ⅰ iquka i-7 DNA bands, i-DNA bands: 100bp (50ng/5μl), 200bp (30ng/5μl), 300bp (30ng/5μl), 400bp (40ng/5μl), 500bp (20ng/0) , 500bp (20ng/5p) bp(35ng/5μl).
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2000bp DNA Ladder
◮ I-2000bp DNA Ladder yimveliso ekulungele ukusetyenziswa kwaye sele iqulethe i-1x yokulayisha i-Buffer.Unokuthatha ngokuthe ngqo inani elifanelekileyo leMarker ye-electrophoresis ngokweemfuno zovavanyo.I-BMNA Marker inamaqela ama-6 ee-DNA, ii-DNA ze-DNA zezi: 100bp (50bp / i-50bp (50bp (50bp / 5μl (50bp / 50b / 5μl (100) (100μl (100μl).
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5000bp DNA Ladder
◮ 2000bp DNA Ladderyimveliso elungele ukusetyenziswa kwaye sele iqulathe i-1x yokuLayisha iBuffer.Unokuthatha ngokuthe ngqo inani elifanelekileyo leMarker ye-electrophoresis ngokweemfuno zovavanyo.I-BM5000 DNA Marker iquka i-8 band DNA, i-DNA bands: 100bp (50ng/5μl), 250bp (50ng/5μl), 500bp (50ng/5μl), 750bp (75ng/5μl), 1000) ngb/5p (500) ngb/5p (5) ), 3000bp (30ng/5μl), 5000bp (50ng/5μl).
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PCR Easyᵀᴹ (ngedayi)
I-2 × PCR HeroTM Inkqubo yokuxuba inokunyamezela okuphezulu kwi-PCR inhibitors kunenkqubo eqhelekileyo ye-PCR Mix, kwaye inokumelana ngokulula nokwandiswa kwe-PCR yeetemplates ezahlukeneyo ezinzima.Inkqubo yokusabela ekhethekileyo kunye ne-Taq Hero ephezulu yenza ukuba i-PCR iphendule ibe nempumelelo ephezulu yokukhulisa, ukuchaneka kunye novakalelo.
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I-PCR Easy
Inkqubo eyodwa yokusabela kunye nokusebenza okuphezulu kwe-Taq DNA Polymerase yenza ukuba i-PCR iphendule ibe nomgangatho ophezulu wokukhulisa, ukuchaneka kunye novakalelo.
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PCR Heroᵀᴹ
◮Ukuthembeka okuphezulu: 6amaxesha ukuba yesiqhelo Taq enzyme;
◮Ngokukhawuleza aisantya sokuphucula:isantya sokukhulisa i-5-10sec/kb, eyi-6-12 amaxesha e-enzyme eqhelekileyo ye-Taq;
◮Ukuziqhelanisa ngakumbi kwetemplate: inokukhulisa ngokufanelekileyo iitemplate ezahlukeneyo ze-DNA ezinomxholo ophezulu we-GC kwaye kunzima ukukhulisa;
◮PhezuluUkwandiswa kobuchule: inani lemijikelo yokwandisa lingaphantsi kunelo le-enzyme yeTaq eqhelekileyo;
◮Sqina Ukunyamezelana kwendalo: ibekwe kuma-37°C kangangeveki, igcina ngaphezu90%umsebenzi;
◮Ino-5'→3' DNA polymerase umsebenzi kunye 5'→3' exonuclease umsebenzi, ngaphandle 3'→5' umsebenzi exonuclease.
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QuickEasyᵀᴹ Ixesha lokwenyani PCR Kit-Taqman
◮Inkqubo ekhethekileyo yokuphucula iPCR yenza i-2×QuickEasyTMReal PCR Mix-Taqman ehambelana ngakumbi.
◮Ukuqala okushushu kwe-Foregene HS Taq Polymerase inomgangatho ophezulu wokukhulisa umvandedwa, uvakalelo oluphezulu lokukhulisa, kunye nokucaciswa okuphezulu kokukhulisa.
◮2× QuickEasyTMI-Real PCR Mix -i-Taqman isebenzisa inkqubo ekhethekileyo eyenziwe yi-Foregene ukuphucula ubuntununtunu kunye neenkcukacha zokufumanisa ulandelelwano oluthile lwe-probe, enokusetyenziswa kwi-genotyping kunye nokukopisha ukuhluka kwenombolo kunye nokufumana iziphumo ezichanekileyo.
◮Le mveliso iza nedayi yesalathiso yangaphakathi ye-ROX, enokusetyenziselwa ukuphelisa imvelaphi yomqondiso kunye nempazamo yomqondiso phakathi kwamaqula, ekulungeleyo ukuba abathengi basebenzise kwiintlobo ezahlukeneyo zezixhobo zePCR zobungakanani.
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