PCR Heroᵀᴹ
Inkcazelo yeKit:
◮Ukuthembeka okuphezulu: 6amaxesha ukuba yesiqhelo Taq enzyme;
◮Ngokukhawuleza aisantya sokuphucula:isantya sokukhulisa i-5-10sec/kb, eyi-6-12 amaxesha e-enzyme eqhelekileyo ye-Taq;
◮Ukuziqhelanisa ngakumbi kwetemplate: inokukhulisa ngokufanelekileyo iitemplate ezahlukeneyo ze-DNA ezinomxholo ophezulu we-GC kwaye kunzima ukukhulisa;
◮PhezuluUkwandiswa kobuchule: inani lemijikelo yokwandisa lingaphantsi kunelo le-enzyme yeTaq eqhelekileyo;
◮Sqina Ukunyamezelana kwendalo: ibekwe kuma-37°C kangangeveki, igcina ngaphezu90%umsebenzi;
◮Ino-5'→3' DNA polymerase umsebenzi kunye 5'→3' exonuclease umsebenzi, ngaphandle 3'→5' umsebenzi exonuclease.
Iinkcazelo Kit
Le mveliso ibandakanya isizukulwana esitsha esikhethekileyo seForegene sikaTaq——Forgene Taq Hero, dNTPs elungiselelweyo, MgCl2, i-reaction buffer, i-PCR reaction enhancer, i-optimizer kunye ne-stabilizer.I-2 × PCR HeroTMInkqubo yokuxuba inokunyamezela okuphezulu kwi-PCR inhibitors kunenkqubo eqhelekileyo ye-PCR Mix, kwaye inokumelana ngokulula nokwandiswa kwe-PCR yeetemplates ezahlukeneyo ezinzima.Inkqubo yokusabela ekhethekileyo kunye ne-Taq Hero ephezulu yenza ukuba i-PCR iphendule ibe nempumelelo ephezulu yokukhulisa, ukuchaneka kunye novakalelo.
Iinkcukacha
Uthotho lweKit
PH-01013: 2× PCR HeroTMXuba 1.7ml ×3
PH-01014: 2× PCR HeroTMXuba 1.7ml × 6
PH-01015: 2× PCR HeroTMXuba 1.7ml ×24
Amacandelo ekhithi
2 × PCR HeroTMUmxube uquka: 0.1U Taq Hero/μl, 400μM dNTPs, 20mM Tris-HCl(pH8.8), 100mM KCl, 5mM MgCl2, ezinye izizinzi kunye nezixhasi.
Iimpawu&izinto eziluncedo
-Ukuthembeka okuphezulu:6amaxesha ukuba yesiqhelo Taq enzyme;
- Ngokukhawuleza aisantya sokuphucula:isantya sokukhulisa i-5-10sec/kb, eyi-6-12 amaxesha e-enzyme eqhelekileyo ye-Taq;
-Ukuziqhelanisa ngakumbi kwetemplate: inokukhulisa ngokufanelekileyo iitemplate ezahlukeneyo ze-DNA ezinomxholo ophezulu we-GC kwaye kunzima ukukhulisa;
- EphakamileyoUkwandiswa kobuchule: inani lemijikelo yokwandisa lingaphantsi kunelo le-enzyme yeTaq eqhelekileyo;
-Sqina Ukunyamezelana kwendalo: ibekwe kuma-37°C kangangeveki, igcina ngaphezu90%umsebenzi;
-Ino-5'→3' DNA polymerase umsebenzi kunye 5'→3' exonuclease umsebenzi, ngaphandle 3'→5' umsebenzi exonuclease.
Isicelo seKit
-Ukwandiswa kwePCR yamaqhekeza eDNA
- DNA ilebhile
- DNA ulandelelwano
-PCR kunye nomsila
Ukugcinwa kunye nobomi beshelufu
Inokugcinwa ixesha elide kwi -20 ° C;ukusetyenziswa rhoqo, inokugcinwa kwi-4 ° C ixesha elifutshane.
