I-Blood DNA Midi Kit (1-5ml) I-Blood Isolation Midi Kits ye-DNA kwiGazi
Inkcazelo yeKit:
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu kwigazi eli-anticoagulated (1-5ml).
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
Inkcazo
Isampuli yegazi iqulethe inani elikhulu leeseli ezibomvu zegazi kunye neeseli zeplatelet, nto leyo eyenza ukuba inkqubo yegazi ibe yinkathazo.Nangona kunjalo, i-DNA ye-genomic yegazi ekhutshwe yindlela eqhelekileyo ayiphezulu ekuhlambulukeni kwaye umxholo uphantsi kakhulu.Le khithi isebenzisa entsha kraca ye-Foregene Protease Plus kunye ne-BL1 eyodwa kunye ne-BL2 isithinteli se-buffer systems, enokwetyisa ngokupheleleyo igazi elichasayo kwayeukujijaiisampulu ngelona xesha lifutshane, ngaloo ndlela kuthintelwa ukuthotywa kwe-DNA kowona mgangatho mkhulu kwaye ufumane elona nani likhulu le-genomic DNA.Inkqubo yokucubungula igazi ngokukhawuleza kunye nokusebenza kwekholamu elula ye-spin kulula kakhulu ukutsalwa kwe-genome yegazi, ukwenzela ukuba i-DNA ye-genomic ephezulu, ecocekileyo ephezulu inokufumaneka kwimizuzu engama-40.
I-DNA-kuphela kwe-silica gel membrane esetyenziswe kwikholamu ye-spin yale kit yinto entsha ekhethekileyo yenkampani, eyenza ngokufanelekileyo kwaye ibhengeze ngokukodwa i-DNA, kwaye inokususa i-RNA, iiprotheni ezingcolileyo, i-ion kunye nezinye izinto eziphilayo kwiiseli ukuya kwinqanaba elikhulu.Iziqwenga ze-DNA ezifunyenweyo zikhulu, ezinobunyulu obuphezulu, obuzinzile kunye nomgangatho othembekileyo, kwaye umthamo omkhulu wokuthwala ikholomu ye-spin yi-80 μg DNA.I-DNA efunyenweyo ingasetyenziselwa imifuniselo yebhayoloji yemolekyuli enjengokuthintelwa kokutya, i-PCR, i-hybridization yaseMazantsi, ukwakhiwa kwethala leencwadi njalo njalo.Ikiti inokuqhubela phambili ukuya kwi-5ml yegazi ngexesha lokufumana inani elaneleyo le-DNA ye-genomic ephezulu.
Iinkcukacha
50 Preps, 100 Preps, 250 Preps
Amacandelo ekhithi
| Isithinteli BL1 |
| Isithinteli BL2* |
| Buffer DC |
| Isithinteli PW* |
| Isithinteli WB1 |
| Isithinteli EB |
| Foregene Protease Plus |
| Ikholamu yeDNA-Kuphela |
| Imiyalelo |
Iimpawu&izinto eziluncedo
-Akukho ungcoliseko lwe-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
-Isantya esikhawulezayo: I-Foregene Protease Plus inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye igaya iisampuli zezicubu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-40.
-Elula: I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4°C iqondo eliphantsi lobushushu becentrifugation okanye i-ethanol precipitation yeDNA.
-Ukhuseleko: Akukho kukhutshwa kwe-organic reagent efunekayo.
-Umgangatho ophezulu: I-DNA ye-genomic ekhutshweyo inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
Isicelo seKit
Ikulungele ukutsalwa kunye nokucocwa kwe-DNA ye-genomic kwigazi elitsha okanye elinomkhenkce elichasene ne-anticoagulated.
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
-Le khithi ingagcinwa kwiinyanga ezili-12 phantsi kweemeko ezomileyo kwindawo yokushisa (15-25).℃);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8℃.
Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yitshise kwindawo yokuhlambela amanzi angama-37 ° C imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.
-Isisombululo se-Forgene Protease Plus sinefomula ekhethekileyo, esebenzayo xa igcinwe kwindawo yokushisa ixesha elide (iinyanga ezi-3), umsebenzi wayo kunye nokuzinza kuya kuba ngcono xa kugcinwe kwi-4 ° C, ngoko kuyacetyiswa ukuba uyigcine kwi-4 ° C, khumbula ukuba ungayigcini -20℃.
