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I-RT Easyᵀᴹ II Master Premix yokuqala-strand cDNA synthesis yeXesha lokwenyani PCR
- Inkqubo esebenzayo yokukhuphela umva, kuthatha imizuzu engama-20 kuphela ukugqiba udityaniso lwe-cDNA yomtya wokuqala.
-I-high-sensitivity reverse transcription system, i-pg-level templates inokufumana i-cDNA ephezulu.
-Isistim yokukhutshelwa umva inozinzo oluphezulu lwe-thermal, iqondo lobushushu lenkqubo linokuba phezulu ukuya kuma-50 ℃, kwaye inomsebenzi olungileyo wokukhutshelwa umva.
- Nkqu neesampulu ze-RNA ezingcolileyo (utywala ukuya kwi-60%, ityuwa ye-guanidine ukuya kwi-750mM) inokuthi ifakwe kwi-reverse transcription reaction.
- 2× RT OKANYE-EasyTM Umxube wongeze iiprimer ezikhutshelweyo eziphuculweyo (Random Primer, Oligo(dT)18 Isiqalo).
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