Iseli ngqo ye-RT qPCR Kit

Iseli ngqo ye-RT qPCR Kit

Inkcazo yeKiti:

Le khithi isebenzisa inkqubo ekhethekileyo ye-lysis buffer enokuthi ikhuphe i-RNA ngokukhawuleza kwiisampulu zeseli ezenzelwe i-RT-qPCR reaction, ngaloo ndlela ishenxisa inkqubo yokuhlanjululwa kwexesha kunye ne-RNA. Itemplate yeRNA inokufumaneka kwimizuzu nje esi-7. Umxube we-5 × ngqo we-RT kunye ne-2 × ngqo qPCR Mix-SYBR reagents ebonelelwe yikiti inokufumana ngokukhawuleza nangokufanelekileyo iziphumo ze-PCR zexesha lokwenyani.

I-5 × i-Direct RT Mix kunye ne-2 × i-Direct QPCR Mix-SYBR ine-inhibitor eyomeleleyo yokunyamezelana, kwaye i-lysate yeesampulu inokusetyenziswa njenge template ye-RT-qPCR ngqo. Le khithi iqulethe i-RNA ekhethekileyo yokuhlangana okuphezulu kwe-Foregene reverse transcriptase, kunye ne-Hot D-Taq DNA polymerase, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR optimizer kunye ne-stabilizer.


Iinkcukacha Product

Iimpawu zemveliso

Ukucaciswa

200×20μI-Rxns, i-1000×20μl IiRxns

Izixhobo zekiti

Icandelo I

IBuffer CL

I-Foregene Protease Plus II

IBuffer ST

Icandelo II

Isicimi seDNA

5 × Umxube we-RT ngqo

2 × ngqo QPCR Mix-SYBR

Idayi yesalathiso ye-50 × ROX

RNase-Simahla ddH2O

Imiyalelo

Iimpawu kunye nezibonelelo

■  Ilula kwaye iyasebenza: ngetekhnoloji ye-Cell Direct RT, iisampulu zeRNA zinokufunyanwa kwimizuzu nje esi-7.

The Imfuno yesampulu incinci, ukuya kuthi ga kuvavanyo lweeseli ezili-10.

■ Ukuhamba okuphezulu: inokukhawuleza ibone i-RNA kwiiseli ezikhuliswe ngo-384, 96, 24, 12, 6, 6-iipleyiti.

■ I-DNA Eraser inokuzisusa ngokukhawuleza iijenome ezikhutshiweyo, ukunciphisa kakhulu ifuthe kwiziphumo zovavanyo ezilandelayo.

■ Ukulungiselela i-RT kunye ne-qPCR inkqubo yenza ukuba amanyathelo amabini e-RT-PCR abuyisele ukukhuphela ngakumbi ngakumbi kwaye i-PCR icace ngakumbi, kwaye inganyangeki kwi-RT-qPCR reaction inhibitors.

Isicelo seKiti

Ubungakanani besicelo: iiseli ezikhulisiweyo.

-I-RNA ekhutshwe sisampulu ye-lysis: isebenza kuphela kwi-template ye-RT-qPCR yale khithi.

-Ikiti inokusetyenziselwa ezi njongo zilandelayo: Uhlalutyo lwentetho yemfuza, ukuqinisekiswa kwe-siRNA-Mediated gene yokuthomalalisa ukusebenza, ukuvavanywa kweziyobisi, njl.

Umzobo

Cell Direct RT qPCR diagram

Ukugcinwa kunye nobomi beShelf

Icandelo I lale khithi kufuneka ligcinwe nge-4 ℃; Icandelo II kufuneka ligcinwe kwi -20 ℃.

 I-Foregene Protease Plus II kufuneka igcinwe ngo-4Ewe, musa ukubanda -20 ℃.

 Reagent 2×I-qPCR Mix-SYBR ngqo kufuneka igcinwe -20ebumnyameni; ukuba isetyenziswa rhoqo, inokugcinwa kwi-4℃ yokugcina okwethutyana (yisebenzise zingaphelanga iintsuku ezili-10).


  • Egqithileyo
  • Okulandelayo:

  • Bhala umyalezo wakho apha uze uyithumele kuthi

    Imveliso iindidi