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Ikiti ye-PCR yeZilwanyana eziNgqo (ngaphandle kweZixhobo zokuSampula) iProtocol
Sebenzisa ngokuthe ngqo izicubu zezilwanyana i-lysate njengethempleyithi yokukhulisa i-PCR, ngaphandle kokutsalwa okwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.
◮Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.
◮Imfuno yesampulu incinci, thatha nje imbewu enye.
◮Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.
◮Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.
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I-Animal Tissue Direct PCR kit-UNG (ngaphandle kweSampuli yeZixhobo) iProtocol
Sebenzisa ngokuthe ngqo izicubu zezilwanyana i-lysate njengethempleyithi yokukhulisa i-PCR, ngaphandle kokutsalwa okwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.Kongezwe inkqubo ye-UNG yokuchasana nokungcoliseka kongcoliseko ukuze kupheliswe iimpawu zobuxoki.
◮Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.
◮Imfuno yesampulu incinci, thatha nje imbewu enye.
◮Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.
◮Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.
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