I-RNAlater (Yokuzinziswa kwe-RNA) I-RNAlater Uzinziso Isisombululo
Inkcazelo yeKit:
Ukulungiswa ngokukhawuleza kwezicubu zesampulu zezilwanyana ezisanda kuthathwa, zithintela umsebenzi we-RNase, zikhusele i-RNA ekuthotyweni.
-Ama-reagents asetyenziswa ngokuthe ngqo, inyathelo elinye likhona, kwaye i-RNA iyazinziswa ngokukhawuleza kwaye ikhuselwe.
-Ukusebenza kwiqondo lobushushu begumbi, kukulungele, kukhuselekile kwaye akukho tyhefu.
-Ukulahla inkathazo yomkhenkce owomileyo okanye ifriji ephantsi yobushushu bokugcina izicubu.
-I-tissue inokugcinwa ixesha elide ngaphandle komngcipheko wokuthotywa kwe-RNA, iqinisekisa idatha yeprofayili ye-gene ethembekileyo.
Iinkcazelo
I-RNAlater lulwelo olungenatyhefu lokugcinwa kweethishu zezilwanyana.Inokungena ngokukhawuleza kwiiseli zezicubu kwaye ikhusele i-RNA yeselula engakhenkcekiyo ekuthotyweni ngokuthintela ngokufanelekileyo umsebenzi we-RNase, ukuze emva kokuba isampulu yethishu ifunyenwe, akufuneki ukuba kusetyenzwe isampuli ngokukhawuleza, kwaye akuyomfuneko ukukhenkceza isampuli kwi-nitrogen engamanzi.,ngokoilunge ngakumbi kwimisebenzi yovavanyo elandelayo.Ukusetyenziswa kwesisombululo sokukhusela i-RNA ye-tissue kunokuthintela ukuphazamiseka kokusebenzisa i-nitrogen engamanzi okanye iifriji ze-ultra-low-low, kunye nokugcina iibhetshi ezahlukeneyo zeesampulu zethishu kwisisombululo sokukhusela kunokumisa ngokukhawuleza kwaye kulungiswe utshintsho olulandelelanayo lwentetho ye-RNA, enokunciphisa impazamo phakathi kwamaqela ovavanyo.
I-RNAlater inokusetyenziswa ngokubanzi kwiisampulu ezahlukeneyo ze-vertebrate, kubandakanya ingqondo, intliziyo, izintso, udaka, isibindi, kunye nemiphunga.Emva kokuba izicubu ezitsha ezingenayo i-frozen zifakwe kwi-Buffer RNAlater kwi-1: 10, iisampuli zingagcinwa kwiqondo lokushisa kwe-1 iveki, i-37 ° C ngosuku lwe-1, i-4 ° C ubuncinane inyanga ye-1;izicubu zinokuntywiliselwa kwi-4 ° C kwi -20 ° C okanyesikrazukile ku -80 ℃ ixesha elide.
Iinkcukacha
50 ml, 100 ml, 100 * 4 ml
Amacandelo ekhithi
| I-RNAkamvaKuzinziso lweRNA | |||
| Ikati.No. | RL-01011 | RL-01012 | RL-01013 |
| Buffer RNAkamva | 50ml | 100ml | 100ml×4 |
| Incwadi Yemiyalelo | 1 iqhekeza | 1 iqhekeza | 1 iqhekeza |
Iimpawu&izinto eziluncedo
-Ama-reagents asetyenziswa ngokuthe ngqo, inyathelo elinye likhona, kwaye i-RNA iyazinziswa ngokukhawuleza kwaye ikhuselwe.
-Ukusebenza kwiqondo lobushushu begumbi, kukulungele, kukhuselekile kwaye akukho tyhefu.
-Ukulahla inkathazo yomkhenkce owomileyo okanye ifriji ephantsi yobushushu bokugcina izicubu.
-I-tissue inokugcinwa ixesha elide ngaphandle komngcipheko wokuthotywa kwe-RNA, iqinisekisa idatha yeprofayili ye-gene ethembekileyo.
Iiparamitha zeKit
I-RNAkamva Isicelo
Isetyenziselwa ukugcina ngokukhawuleza izicubu zezilwanyana ezisanda kuqokelelwa kwaye uzinzise ngoko nangoko i-RNA kwiisampulu zethishu ukugcina iiprofayili zokubonakaliswa kofuzo.
Iimeko zokugcina
Inokugcinwa ngokuzinzileyo ngaphezu konyaka omnye kwiqondo lobushushu begumbi (15-25℃).
