I-RT Easyᵀᴹ I Master Premix ye-cDNA yomtya wokuqala
Inkcazelo yeKit:
- Inkqubo esebenzayo yokukhuphela umva, kuthatha imizuzu engama-25 kuphela ukugqiba udityaniso lwe-cDNA yomtya wokuqala.
-I-high-sensitivity reverse transcription system, i-pg-level templates inokufumana i-cDNA ephezulu.
-Inkqubo yokukhuphela umva inozinzo oluphezulu lwe-thermal, ubushushu bokusabela kwenkqubo bunokuphakama ukuya kuma-50 ℃,kwaye inomsebenzi olungileyo wokukhuphela umva.
- Nkqu neesampuli ze-RNA ezingcolileyo (utywala ukuya kwi-45%, ityuwa ye-guanidine ukuya kwi-750mM) inokuthi ifakwe kwi-reverse transcription reaction.
Iinkcazelo Kit
FOREGENE kaThixo 2× RT EasyTMUmxube unokudibanisa i-cDNA yokuqala-strand kwiqondo lokushisa elifikelela kuma-50 ° C, elilungele ukuphendulwa kwe-reverse transcription ye-complex complex structure RNA templates.
I-RNA ehlanjululweyo kwilabhoratri ihlala iqulethe i-alcohol kunye ne-guanidine iintsalela zetyuwa, ezineepropati ezinamandla zokuthintela kwi-reverse transcriptases, ezikhokelela kwimiphumo enganelisiyo yokukhuphela i-reverse okanye ukusebenza kakuhle kwe-reverse transcription.FOREGENE 2 × RT EasyTMUkuxuba kubonisa ukunyamezela okuphezulu kakhulu kutywala kunye netyuwa ye-guanidine, ukunyamezela okuphezulu kotywala kwiisampuli ze-RNA yi-45%, kwaye ukunyamezela okuphezulu kwi-guanidine ityuwa yi-750mM.Nangona i-RNA ayihlambulukanga, ukuphendulwa kwe-reverse transcription kungenziwa nge-FOREGENE 2 × RT Mix.Inkqubo yokusabela eyodwa yenza i-RT reaction ibe lula, ikhawuleza kwaye isebenze ngakumbi, kwaye ukuhlanganiswa kwe-cDNA yomcu wokuqala kunokugqitywa kwimizuzu engama-25.
Le mveliso inokusetyenziswa ngokudityaniswa ne-Foregene's fluorescent quantitative product Real Time PCR EasyTMukufumana iziphumo zovavanyo ezikumgangatho ophezulu.
Iinkcukacha
| RT EasyTMI I-Master Premix ye-first-strand cDNA synthesis | ||
| Imixholo yekhithi | RT-01011 | RT-01012 |
| 25 amaxesha (20μl inkqubo) | 100 amaxesha (20μl inkqubo) | |
| I-2 × i-RT Easy TM Mix | 0.25ml | 1ml |
| I-Primer Random (50μM) | 50μl | 200μl |
| I-Oligo(dT)18 Primer (50μM) | 50μl | 200μl |
| RNase-Free ddH2O | 1.7ml | 1.7ml |
| Incwadi Yemiyalelo | Iqhekeza eli-1 | Iqhekeza eli-1 |
Iimpawu&izinto eziluncedo
- Inkqubo esebenzayo yokukhuphela umva, kuthatha imizuzu engama-25 kuphela ukugqiba udityaniso lwe-cDNA yomtya wokuqala.
-I-high-sensitivity reverse transcription system, i-pg-level templates inokufumana i-cDNA ephezulu.
-Isistim yokukhutshelwa umva inozinzo oluphezulu lwe-thermal, iqondo lobushushu lenkqubo linokuba phezulu ukuya kuma-50 ℃, kwaye inomsebenzi olungileyo wokukhutshelwa umva.
- Nkqu neesampuli ze-RNA ezingcolileyo (utywala ukuya kwi-45%, ityuwa ye-guanidine ukuya kwi-750mM) inokuthi ifakwe kwi-reverse transcription reaction.
Isicelo seKit
- I-RT-PCR eqhelekileyo
- Ukwenziwa kwe-cDNA yokwenziwa kwekloni kunye nokubonakalisa uphando
- Uhlalutyo lwendlela yokwandiswa kwe-primer yesayithi yokuqaliswa koshicilelo
- RACE (ukukhulisa ngokukhawuleza kwe-cDNA iphela)
-Ukwandiswa kweRNA ngomgca
- Uphawu lweChip
Ukuhamba komsebenzi
Ukugcinwa kunye nobomi beshelufu
1.Iimeko zothutho
Yonke inkqubo yokuthutha i-ice box yobushushu obuphantsi, ukuqinisekisa ukuba ikiti ikwimo ye-<4 ℃.
2. Iimeko zokugcina
RT EasyTMNdigcinwa kwi -20°C.Gcina imveliso kwifriji ehlala rhoqo kwi-20 ° C ngokukhawuleza emva kokufunyanwa.Ukuba iimeko zokugcina zifanelekile, imveliso ayiyi kuthoba nayiphi na intsebenzo ngexesha le-1 leminyaka yokuqinisekisa.
