I-PCR yokucoca iKhiti ye-PCR yokuCoca-up System ye-Ultra-pure DNA
Inkcazelo yeKit:
Ngokukhawuleza uhlambulule kwaye ufumane amaqhekeza e-DNA akumgangatho ophezulu kwinkqubo yePCR;ukuba ufuna ukwahlula kwaye ufumane amaqhekeza athile eDNA, nceda ukhethe ikhithi yokubuyisela ijeli.
◮ Uluhlu olubanzi lokuchacha kwe-DNA: Iziqwenga ze-DNA zifutshane njenge-60bp kwaye ezinkulu njenge-10kb zinokufunyanwa.
◮ Ukusebenza okuphezulu kokubuyisela: Ukusebenza kokubuyisela ngokuqhelekileyo kungaphezulu kwe-80%, kwaye iphakamileyo inokufikelela ngaphezu kwe-95%.
◮ Isantya esiphezulu:Kulula ukuyisebenzisa, ukubuyiswa kweqhekeza le-DNA kunokugqitywa kwimizuzu eyi-15 kwaye i-primer dimer inokususwa ngokuthe ngqo ngaphandle kokubuyiswa kwe-gel.
◮ Safety:Akukho kutsalwa kwe-organic reagent efunekayo.
◮ Umgangatho ophezulu: Amaqhekeza e-DNA afunyenweyo acoceke kakhulu, anokudibana novavanyo olwahlukeneyo olulandelayo.
Inkcazo
Le khithi isebenzisa i-spin ikholamu kunye nefomula ephuhliswe yinkampani yethu, enokubuyisela ngokufanelekileyo amaqhekeza e-DNA acoceke kakhulu kwinkqubo yePCR.Ikholamu yokucoca inokubopha ngokufanelekileyo i-DNA, kwaye ihambelana ne-reagent ye-formula ekhethekileyo yokususa ngokuthe ngqo i-primer dimer ngaphandle kokubuyisela i-gel.
Ikhithi ibuyisela uluhlu olubanzi lwamaqhekeza eDNA.Ngaphantsi kweemeko eziqhelekileyo, iziqwenga ze-DNA ezimfutshane njenge-60bp zinokufunyanwa.Ubuncinci be-30μl ye-eluent ingasetyenziselwa ukunyusa uxinaniso lwe-DNA efunyenweyo.
Ikhithi kulula ukuyisebenzisa kwaye inamanyathelo ambalwa.Ifuna kuphela i-centrifugation emininzi ukucubungula iisampuli ezininzi ngexesha elinye, kwaye iziqwenga ze-DNA ezicocekileyo ezifunyenweyo zinokufunyanwa kwimizuzu eyi-15.
Iinkcukacha
50 Preps, 100Preps, 250Preps
Amacandelo ekhithi
| Isithinteli BD* |
| Isithinteli BD-S |
| Isithinteli WB1 |
| Isithinteli EB |
| Ikholamu yeDNA-Kuphela |
| Imiyalelo |
Iimpawu&izinto eziluncedo
-Uluhlu olubanzi lokubuyiswa kwe-DNA: Iziqwenga ze-DNA zifutshane njenge-60bp kwaye ezinkulu njenge-10kb zinokufunyanwa.
-Ukuphumelela okuphezulu kokubuyisela: Ukusebenza kakuhle kokubuyisela ngokuqhelekileyo kungaphezulu kwe-80%, kwaye iphakamileyo inokufikelela ngaphezu kwe-95%.
-Isantya esikhawulezayo: Kulula ukusebenza, ukubuyiswa kweqhekeza le-DNA kunokugqitywa kwimizuzu eyi-15 kwaye i-primer dimer inokususwa ngokuthe ngqo ngaphandle kokubuyiswa kwe-gel.
-Ukhuseleko: Akukho kukhutshwa kwe-organic reagent efunekayo.
-Umgangatho ophezulu: Iziqwenga ze-DNA ezifunyenweyo zicocekile kakhulu, ezinokuhlangabezana neemvavanyo ezahlukeneyo ezilandelayo.
Isicelo seKit
Le kit ifanelekile ukubuyisela amaqhekeza e-DNA (60bp-10kb) kwiimveliso ze-PCR.
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
Le khithi ingagcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo kwiqondo lokushisa (15-25 ° C);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8 ° C.
Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yitshise kwindawo yokuhlambela amanzi angama-37 ° C imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.
