1kb DNA Ileli
Inkcazelo yeKit:
◮I-1Kb DNA Ladder iqulethwe ngamacandelo amabini e-DNA, kwaye i-concentration ephindwe kabini ye-1kb kunye ne-5kb inceda ukuhlukanisa amaqela ahlukeneyo.Ngaphantsi kwe-1kb, iibhendi ezintathu ze-250bp, 500bp, kunye ne-750bp zongezwa, kwaye ubungakanani beqhekeza elifutshane lingabalwa ngokususela kule nto.Ukugxininiswa kweebhendi ezine ze-6kb, 7kb, 8kb, kunye ne-10kb ziqingqiwe, eziluncedo ukufumana umphumo ongcono wokuhlukana.
Iinkcazelo
I-1Kb DNA Ladder iqulethwe ngamacandelo amabini e-DNA, kwaye i-concentration ephindwe kabini ye-1kb kunye ne-5kb inceda ukuhlukanisa amaqela ahlukeneyo.Ngaphantsi kwe-1kb, iibhendi ezintathu ze-250bp, 500bp, kunye ne-750bp zongezwa, kwaye ubungakanani beqhekeza elifutshane lingabalwa ngokususela kule nto.Ukugxininiswa kweebhendi ezine ze-6kb, 7kb, 8kb, kunye ne-10kb ziqingqiwe, eziluncedo ukufumana umphumo ongcono wokuhlukana.
Le mveliso sele iqulethe i-1× Iyalayisha iBuffer.Ungathatha i-2-6μl ye-electrophoresis ngokuthe ngqo ngokweemfuno zovavanyo.Umfanekiso we-electrophoresis ucacile, ulungele kwaye usebenza.
Iibhanti ezili-13 zale mveliso ukusuka kwincinci ukuya enkulu yile: 250bp, 500bp, 750bp, 1kb, 1.5kb, 2kb, 3kb, 4kb, 5kb, 6kb, 7kb, 8kb, 10kb.Ukuba umthamo wokulayisha ngu-5μl, i-1kb kunye ne-5kb bands ziyi-100ng;6kb, 7kb, 8kb, kunye 10kb bands are 25ng;kwaye amabhanti aseleyo angama-50ng.
Iinkcukacha
| DL-30011 | Amalungiselelo angama-50 (250μl) |
| DL-30012 | 200 Preps (1ml) |
Amacandelo ekhithi
Ulwazi lolwelo lokugcina
10mM Tris-HCl(pH 8.4) 10mM EDTA
0.02% BPB
I-5% ye-glycerinum
Ugxininiso lwemveliso
Uxinzelelo lwemveliso malunga ne-130μg / ml
Electropherogram
Iimeko zokugcina
Nceda ugcine ngo-4℃(nceda ugcine kwi-20℃ukugcina ixesha elide)
