I-RT-PCR Easyᵀᴹ I(Inyathelo elinye)
Inkcazelo yeKit:
◮Ikiti yenyathelo elinye yenza ukuba uguqulelo loguqulelo kunye nePCR luqhutywe kwityhubhu enye.Ifuna kuphela ukongeza ithemplate ye-RNA, iiprimers ezithile zePCR kunye ne-RNase-Free ddH2O.
◮Uhlalutyo lwexesha langempela lobungakanani be-RNA lunokwenziwa ngokukhawuleza nangokuchanekileyo.
◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
◮Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
Iinkcukacha
Foregene One-Inyathelo RT-PCR EasyTM iimveliso zechungechunge ziqonda ukusabela okudityanisiweyo ukusuka kwi-RNA ukuya kwi-DNA ephindwe kabini, oko kukuthi, ukukhutshelwa umva kunye nokukhulisa i-PCR kugqitywe kwityhubhu efanayo yokusabela ye-centrifuge kunye nenkqubo yokusabela efanayo, eyenza lula amanyathelo okulinga., kwandisatyena inkqubo yovavanyo, kwayeiyaphucuka umfuniselo ukusebenza kakuhle.
RT-PCR EasyTM I(Inyathelo elinye) yikiti ye-RT-PCR enenyathelo elinye ephuhliswe ngokukodwa yi-Foregene, eqaphela ukusebenza okuqhubekayo ukusuka kwi-RT ukuya kwi-PCR kwityhubhu enye.Umsebenzi ulula kwaye uyakhawuleza, onokunciphisa ngokufanelekileyo ungcoliseko ngexesha lovavanyo.2×RT-PCR EasyTM Umxube unokunyamezela okuqinileyo, ukuzinza kwe-thermal, ukusebenza kakuhle kunye nokucacileyoity for ukukhulisa.
Amacandelo ekhithi
| RT-PCR EasyTMMna (Inyathelo elinye) | ||
| Imixholo yekhithi | RT-02011 | RT-02012 |
| 100T (20μl inkqubo) | 500T (20μl inkqubo) | |
| 2 × RT-PCR EasyTM Xuba | 1ml | 1.7ml×3 |
| RNase-Free ddH2O | 1.7ml | 1.7ml×3 |
| Incwadi Yemiyalelo | Iqhekeza eli-1 | Iqhekeza eli-1 |
Iimpawu&izinto eziluncedo
- Ikhithi yenyathelo elinye yenza ukuba uguqulelo lwe-reverse kunye ne-PCR iqhutywe kwi-tube efanayo.Ifuna kuphela ukongeza ithemplate ye-RNA, iiprimers ezithile zePCR kunye ne-RNase-Free ddH2O.
- Uhlalutyo lwexesha langempela lobungakanani be-RNA lunokwenziwa ngokukhawuleza nangokuchanekileyo.
- Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
-Inkqubo yokusabela eyongeziweyo yenza ukusabela kube novakalelo oluphezulu lokubona, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
1.Iimeko zothutho
Yonke inkqubo yothutho lwebhokisi ye-ice box yobushushu obuphantsi, ukuqinisekisa ukuba ikhithi ikwimo ye-<4 °C.
2. Iimeko zokugcina
Ikhithi igcinwa kwi -20°C.Gcina imveliso kwifriji ehlala rhoqo kwi-20 ° C ngokukhawuleza emva kokufunyanwa.Ukuba iimeko zokugcina zifanelekile,timveliso yakhe ayiyi kuthoba nayiphi na intsebenzo ngexesha le-1 leminyaka yokuqinisekisa.
