I-RT-qPCR Easyᵀᴹ (Inyathelo elinye)-SYBR Green I
Inkcazelo yeKit:
◮Ikhithi enenyathelo elinye yenza ukukhutshelwa umva kunye ne-qPCR iimpendulo ezimbini kwityhubhu enye, idinga kuphela ukongeza i-template ye-RNA, iiprimers ezithile ze-PCR kunye ne-RNase-Free ddH.2O.
◮Ikhithi inokukhawuleza kwaye ngokufanelekileyo ihlalutye i-RNA yentsholongwane okanye ilandele i-RNA.
◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
◮Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
◮RT-qPCR EasyTM(Inyathelo elinye) -SYBR Green I kit iza ne-ROX yedayi yesalathiso yangaphakathi, engasetyenziselwa ukuphelisa imvelaphi yomqondiso kunye neempazamo zesignali phakathi kwamaqula, ekulungele ukuba abathengi basebenzise kwiimodeli ezahlukeneyo zezixhobo ze-PCR zobungakanani.
Iinkcazelo
RT-qPCR EasyTM (Inyathelo elinye)-SYBR Green I isebenzisa inkqubo ekhethekileyo yokudibanisa ngokufanelekileyo i-RT kunye ne-Real Time ye-PCR reactions, enokugqiba ngokukhawuleza ukufumanisa ubuninzi beejeni.Imveliso inokunyamezela okuqinileyo, ukuchaneka okuphezulu kunye nokuzinza.Imveliso iqulethe i-Foregene HotStar Taq DNA Polymerase yenkampani ekhethekileyo, enenzuzo yokwandisa ngokukhawuleza (2Kb / min), ukusebenza kakuhle kokukhulisa, amandla okukhulisa amandla athile, kunye nesantya esisezantsi sokungafani xa kuthelekiswa ne-enzymes ye-Taq eqhelekileyo.Isetyenziswe apha kwiXesha lokwenyani i-RT-PCR ukunciphisa ukukhulisa okungangqalanga kunye nokuphucula ukuchaneka kwe-PCR.
Iinkcukacha
100 T (20μl inkqubo)
500 T (20μl inkqubo)
Amacandelo ekhithi
| RT-qPCR EasyTM(Inyathelo elinye)-SYBR Green I | ||
| Imixholo yeKit | RT-02111 | RT-02112 |
| 100T (20μl inkqubo) | 500T (20μl inkqubo) | |
| 2× RT-qPCR EasyTM Umxube-SYBR | 1ml | 1.7ml×3 |
| I-50 × i-ROX Reference Dye | 200μl | 1ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml×3 |
| Incwadi Yemiyalelo | Iqhekeza eli-1 | Iqhekeza eli-1 |
Iimpawu&izinto eziluncedo
-Ikiti enenyathelo elinye yenza ukukhutshelwa okubuyela umva kunye ne-qPCR iimpendulo ezimbini kwityhubhu enye, kufuneka kuphela ukongeza ithemplate ye-RNA, iiprimers ezithile zePCR kunye neRNase-Free ddH.2O.
- Ikhithi inokukhawuleza kwaye ngokufanelekileyo ukuhlalutya i-RNA yentsholongwane okanye ilandele i-RNA.
- Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
-Inkqubo yokusabela eyongeziweyo yenza ukusabela kube novakalelo oluphezulu lokubona, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
- RT-qPCR EasyTM(Inyathelo elinye) -SYBR Green I kit iza ne-ROX yedayi yesalathiso yangaphakathi, engasetyenziselwa ukuphelisa imvelaphi yomqondiso kunye neempazamo zesignali phakathi kwamaqula, ekulungele ukuba abathengi basebenzise kwiimodeli ezahlukeneyo zezixhobo ze-PCR zobungakanani.
Ukuhamba komsebenzi
Iimeko zokugcina
1.Ezothuthoiimeko
Yonke inkqubo yokuthutha i-ice box yobushushu obuphantsi, ukuqinisekisa ukuba ikiti ikwimo ye-<4 ℃.
2.Ugcino iimeko
Ikhithi igcinwe ku -20 ℃.Gcina imveliso kwifriji yokushisa rhoqo kwi-20℃ kwangoko emva kokufunyanwa.Ukuba iimeko zokugcina zifanelekile, imvelisoayisayi kuthoba umgangatho wokwenziwa komsebenzi kwisithuba sonyaka o-1 wokuqinisekisa.
