I-RT-PCR Easyᵀᴹ II(Amanyathelo amabini)
Inkcazelo yeKit:
◮Uhlalutyo lwexesha langempela lobungakanani be-RNA lunokwenziwa ngokukhawuleza nangokuchanekileyo.
◮Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
◮ Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
Iinkcukacha
RT-PCR EasyTM II(Amanyathelo amabini) iqulethe zonke ii-reagents zamanyathelo amabini e-RT-PCR, ukudibanisa i-RT reaction kunye ne-PCR reaction kwikhithi efanayo, ukubonelela nge-RT Mix kunye ne-PCR Mix.Umxube we-RT owahlukileyo unokudibanisa umgangatho ophezulu we-cDNA yokuqala ngokulula nangokufanelekileyo. 2× I-PCRKululaTM XubaUkongeza inomgangatho ophezulu kunye nolwandiso oluthile.Indibaniselwano yendalo yezi zimbini yenza ubhaqo lwejini ekujoliswe kuyo lube novakalelo ngakumbi.
Amacandelo ekhithi
| I-RT-PCRKululaTM II(Amanyathelo amabini) | ||
| Imixholo yekhithi | RT-02021 | RT-02022 |
| 100T(20μlinkqubo) | 500T(20μlinkqubo) | |
| 2× RT EasyTM Xuba | 1ml | 1.7ml×3 |
| 2× I-PCRKululaTM XubaUkongeza | 1ml | 1.7ml×3 |
| I-Primer engaqhelekanga (50μM) | 200μl | 1ml |
| I-Oligo(dT)18 Primer (50μM) | 200μl | 1ml |
| RNase-Free ddH2O | 1.7ml | 1.7ml |
| Incwadi Yemiyalelo | 1iqhekeza | 1iqhekeza |
Iimpawu&izinto eziluncedo
-Uhlalutyo lwexesha langempela lobungakanani be-RNA lunokwenziwa ngokukhawuleza nangokuchanekileyo.
-Ikhithi isebenzisa i-reagent ekhethekileyo ye-Foregene reverse transcription reagent kunye ne-Foregene HotStar Taq DNA Polymerase edityaniswe nenkqubo yokusabela ekhethekileyo ukuze kuphuculwe ngokufanelekileyo ukusebenza kakuhle kokukhulisa kunye neenkcukacha zokuphendula.
-Inkqubo yokusabela eyongeziweyo yenza ukuba impendulo ibe novakalelo oluphezulu lobhaqo, uzinzo olomeleleyo lwe-thermal, kunye nokunyamezela okungcono.
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
1.Iimeko zothutho
Yonke inkqubo yothutho lwebhokisi ye-ice box yobushushu obuphantsi, ukuqinisekisa ukuba ikhithi ikwimo ye-<4 °C.
2. Iimeko zokugcina
Ikhithi igcinwa kwi -20°C.Gcina imveliso kwifriji ehlala rhoqo kwi-20 ° C ngokukhawuleza emva kokufunyanwa.Ukuba iimeko zokugcina zifanelekile,timveliso yakhe ayiyi kuthoba nayiphi na intsebenzo ngexesha le-1 leminyaka yokuqinisekisa.
