I-TP53/CSP17 iProbe yoMbala emibini
Inkcazelo yeKit:
Ngokomgaqo-siseko we-DNA yokudibanisa okuhambelanayo, i-TP53 i-orenji-red probe kunye ne-CSP17 eluhlaza iprobe zisetyenziselwe ukuxutywa kunye nokulandelelana kwethagethi ye-DNA kwi-nucleus, kunye nolwazi lwesimo sofuzo kwi-nucleus lwabonwa kwaye lwahlalutywa phantsi kwe-microscope ye-fluorescence.
Inkcazo
Ngokomgaqo-siseko we-DNA yokudibanisa okuhambelanayo, i-TP53 i-orenji-red probe kunye ne-CSP17 eluhlaza iprobe zisetyenziselwe ukuxutywa kunye nokulandelelana kwethagethi ye-DNA kwi-nucleus, kunye nolwazi lwesimo sofuzo kwi-nucleus lwabonwa kwaye lwahlalutywa phantsi kwe-microscope ye-fluorescence.
Amacandelo ekhithi
| Icandelo | Iinkcukacha | ||
| Iimvavanyo ezi-5 | Iimvavanyo ezili-10 | Iimvavanyo ezingama-20 | |
| I-TP53/CSP17 iProbe yoMbala emibini | 50μl | 100μl | 200μl |
Isicelo seKit
Le khithi isetyenziselwa ukubona ngokusemgangathweni ukungaqhelekanga kwejini ye-TP53 yomntu kwiisampulu ze-cytological okanye iisampulu ze-parafini ezifakwe kwi-vitro.
Iimfuno zesampulu
- Umzekelo wezicubu
Imizekelo yethishu efakwe kwiparafini yalungiswa nge-10% engathathi hlangothi ibuffered formalin, kwaye ubukhulu besilayi babuphakathi kwe-3 kunye ne-5 µm.
- Umzekelo weseli
Kumongo wethambo okanye iisampuli zegazi ze-peripheral, iisampulu ezintsha ezingalungiswanga kufuneka zigcinwe kwi-4 ° C ngaphandle kweeyure ezingama-24, kunye nokumiswa kweseli emiselweyo kufuneka kugcinwe kwi -20 ° C.
Ugcino kunye nobomi beShelf
Igcinwe ku -20℃±5℃ ebumnyameni, isebenza iinyanga ezili-12.Ezothutho ngaphantsi kwe-8 ℃.
