I-CDKN2A/CSP9 yoMbala oMbini weProbe
Inkcazelo yeKit:
I-Fluorescence in situ hybridization (INHLANZI) isekelwe kumgaqo wokudibanisa okuhambelanayo kweziseko ze-DNA, kunye nokubonwa kwemiqondiso ye-hybridization ye-fluorescence-ileyibhile i-DNA probes kunye nokulandelelana kwayo ekujoliswe kuyo kwi-cell nucleus phantsi kwe-microscope ye-fluorescence.
Inkcazo
I-Fluorescence in situ hybridization (INHLANZI) isekelwe kumgaqo wokudibanisa okuhambelanayo kweziseko ze-DNA, kunye nokubonwa kwemiqondiso ye-hybridization ye-fluorescence-ileyibhile i-DNA probes kunye nokulandelelana kwayo ekujoliswe kuyo kwi-cell nucleus phantsi kwe-microscope ye-fluorescence.
Amacandelo ekhithi
| Icandelo | Iinkcukacha | ||
| 5Iimvavanyo | 10Iimvavanyo | 20Iimvavanyo | |
| I-CDKN2A/CSP9 yoMbala oMbini weProbe | 50μl | 100μl | 200μl |
Isicelo seKit
Le khithi isetyenziselwa ukufumanisa ngokusemgangathweni ukungaqhelekanga kwe-CDKN2A yemfuza yabantu kwiisampulu ze-cytological okanye iisampulu ze-parafini ezifakwe kwi-vitro.
Iimfuno zesampulu
- Umzekelo wezicubu
Imizekelo yethishu efakwe kwiparafini yalungiswa nge-10% engathathi hlangothi ibuffered formalin, kwaye ubukhulu besilayi babuphakathi kwe-3 kunye ne-5 µm.
- Umzekelo weseli
Kumongo wethambo okanye iisampuli zegazi ze-peripheral, iisampulu ezintsha ezingalungiswanga kufuneka zigcinwe kwi-4 ° C ngaphandle kweeyure ezingama-24, kunye nokumiswa kweseli emiselweyo kufuneka kugcinwe kwi -20 ° C.
Ugcino kunye nobomi beShelf
Igcinwe ku -20℃±5℃ ebumnyameni, isebenza iinyanga ezili-12.Ezothutho ngaphantsi kwe-8 ℃.
