I-MYC / IGH yoMbala oMbini weProbe
Inkcazelo yeKit:
Ngokomgaqo wesiseko se-DNA sokudibanisa okuhambelanayo, i-MYC ebomvu-orenji probe kunye ne-IGH eluhlaza eprobe zisetyenziselwe ukwenza umxube kunye nolandelelwano lwethagethi ye-DNA kwinucleus, kunye nolwazi lwemeko yemfuza kwinucleus yajongwa kwaye yahlalutywa phantsi kwemakroskopu ye-fluorescence.
Inkcazo
Ngokomgaqo wesiseko se-DNA sokudibanisa okuhambelanayo, i-MYC ebomvu-orenji probe kunye ne-IGH eluhlaza eprobe zisetyenziselwe ukwenza umxube kunye nolandelelwano lwethagethi ye-DNA kwinucleus, kunye nolwazi lwemeko yemfuza kwinucleus yajongwa kwaye yahlalutywa phantsi kwemakroskopu ye-fluorescence.
Amacandelo ekhithi
| Icandelo | Iinkcukacha | ||
| Iimvavanyo ezi-5 | Iimvavanyo ezili-10 | Iimvavanyo ezingama-20 | |
| MYC/IGHiProbe yeMbala eMabini | 50μl | 100μl | 200μl |
Isicelo seKit
Le khithi isetyenziselwa ukukhangela ngokomgangatho ukungaqhelekanga kwejini ye-MYC/IGH yomntu kwiisampulu ze-cytological okanye iisampulu zethishu ezifakwe kwiparafini kwi-vitro.
Iimfuno zesampulu
-Izicubu umzekelo
Imizekelo yethishu efakwe kwiparafini yalungiswa nge-10% engathathi hlangothi ibuffered formalin, kwaye ubukhulu besilayi babuphakathi kwe-3 kunye ne-5 µm.
-Iseli umzekelo
Kumongo wethambo okanye iisampuli zegazi ze-peripheral, iisampulu ezintsha ezingalungiswanga kufuneka zigcinwe kwi-4 ° C ngaphandle kweeyure ezingama-24, kunye nokumiswa kweseli emiselweyo kufuneka kugcinwe kwi -20 ° C.
Ugcino kunye nobomi beShelf
Igcinwe ku -20℃±5℃ ebumnyameni, isebenza iinyanga ezili-12.Ezothutho ngaphantsi kwe-8 ℃.
