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EndoFree Maxi Plasmid Kit (Spin Column)

Inkcazelo yeKit:

Yonke inkqubo yokutsalwa ithatha iyure enye kuphela, iqinisekisa ukusebenza ngokufanelekileyo nangokukhawulezayo.

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Iinkcukacha zeMveliso

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Inkcazo

I-EndoFree Maxi Plasmid Kit isebenzisa ubuchule obukhethekileyo be-silica membrane adsorption ukuze ibophe ngokufanelekileyo kwaye ngokukodwa i-plasmid DNA.Ngokudibanisa i-Endotoxin ekhethekileyo yokususa i-Buffer P4 kunye ne-EndoFree Maxi Filtration Columns, i-endotoxin, iiprotheni kunye nezinye izinto ezingcolileyo zingasuswa ngokufanelekileyo.Yonke inkqubo yokutsalwa ithatha iyure enye kuphela, iqinisekisa ukusebenza ngokufanelekileyo nangokukhawulezayo.

Isixa sebhaktheriya esicetyiswayo kwisenzo ngasinye: Kwi-plasmid ephezulu, kuyacetyiswa ukuba kusetyenziswe i-100 ml yemidiya yenkcubeko yeebhaktheriya, kunye nesivuno esihlala sijikeleze i-500-1500 μg.Kwi-plasmid enekopi ephantsi, i-200 ml yemidiya yenkcubeko yeebhaktheriya iyacetyiswa ukuba yenze malunga ne-200-600 μg plasmids.

Amacandelo ekhithi

Imveliso Amacandelo

D909-E(10 preps)
Isithinteli BL 30 ml
Isithinteli P1 100 ml 
Isithinteli P2 100 ml 
Isithinteli P4 100 ml
Isithinteli DW2 44 ml
Isithinteli EB 30 ml
I-RNase A(10 mg/ml) 1 ml
DNAPure Maxi Spin Columns 10 iipcs
Isirinji yesihluzi seFinePure Maxi 10 iipcs
50 ml Imibhobho yoQokelelo 10 iipcs

Ukufunyanwa kwePlasmid DNA Concentration and Purity

Ukucoceka kunye nokugxininiswa kwe-plasmid DNA ekhutshweyo inokubonwa nge-agarose gel electrophoresis kunye ne-ultraviolet spectrophotometer.Ixabiso le-OD260 le-1 lihambelana malunga ne-50 μg / ml ye-DNA ephindwe kabini.Ixabiso le-OD260 / OD280 ye-DNA ye-plasmid ecocekileyo ngokuqhelekileyo kuluhlu lwe-1.7-1.9.I-DNA ye-plasmid ecocekileyo inokusetyenziswa ngokuthe ngqo kwiimvavanyo ezineemfuno eziphezulu zokucoceka, ezifana nokudluliselwa kweeseli okanye kwiimvavanyo ze-vivo.

Isicelo seKit

I-plasmid DNA ekhutshwe yile kiti ingasetyenziselwa kwimisebenzi eyahlukeneyo yesiqhelo, kubandakanywa ukugaywa kwe-enzymatic, i-PCR, ukulandelelana, i-ligation, ukuguqulwa kunye nokudluliselwa kweentlobo ezahlukeneyo zeeseli.

Ugcino kunye nobomi beShelf

I-EndoFree Maxi Plasmid Kit ingagcinwa kwiqondo lokushisa (15-25 ° C) phantsi kweemeko ezomileyo ukuya kwiinyanga ezili-12.Yongeza i-RNase A kwi-Buffer P1 phambi kokusetyenziswa kokuqala, xuba kakuhle kwaye ugcine kwi-2-8°C.Umxube unokugcinwa ngokuzinzileyo ngaphezu kweenyanga ezi-6.

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