Gel Extraction Kit DNA Gel Extraction Kit
Inkcazelo yeKit:
Ngokukhawuleza nangempumelelo buyisa amaqhekeza e-DNA angama-20bp-10kb kwijeli yeagarose.
◮Uluhlu olubanzi lokubuyiselwa kweDNA:Iziqwenga ze-DNA ezimfutshane njenge-30bp kwaye ezinkulu njenge-10kb zinokufunyanwa.
◮Ukusebenza okuphezulu kokubuyisela:Ukusebenza okuphezulu kokubuyisela kunokufikelela ngaphezu kwe-80%.
◮Inkqubo encinci elution:ubuncinane 30μl elution isisombululo ingasetyenziselwa elution, nto leyo enokuthi ngempumelelo ukunyusa uxinaniso amaqhekeza wachacha DNA.
◮Isantya esiphezulu:Kulula ukuyisebenzisa, ukubuyiswa kweqhekeza le-DNA kunokugqitywa kwimizuzu eyi-15.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:Amaqhekeza e-DNA afunyenweyo acoceke kakhulu, anokudibana nemifuniselo eyahlukeneyo elandelayo.
Inkcazo
Le khithi isebenzisa ikholamu yokujikeleza kunye nefomula ephuhliswe yinkampani yethu, enokubuyisela ngokufanelekileyo amaqhekeza e-DNA acocekileyo kwijeli ye-agarose.Ikhithi inokubuyisela amaqhekeza eDNA kuluhlu olubanzi.Ngaphantsi kweemeko eziqhelekileyo, iziqwenga ze-DNA ze-30bp-10kb zinokufunyanwa.Ubuncinci be-30μl ye-eluent ingasetyenziselwa ukunyusa uxinaniso lwe-DNA efunyenweyo.
Ikhithi kulula ukuyisebenzisa kwaye inamanyathelo ambalwa.Ifuna kuphela i-centrifugation emininzi ukucubungula iisampuli ezininzi ngexesha elinye, kwaye iziqwenga ze-DNA ezicocekileyo ezifunyenweyo zinokufunyanwa kwimizuzu eyi-15.
Iinkcukacha
50 Preps
I-Buffer GE: Nciphisa ibhloko yejeli kwi-60 ° C ukukhulula amaqhekeza e-DNA kwijeli.
I-Buffer WB1: Susa iiyoni zetyuwa ezishiyekileyo kwi-DNA.
I-Buffer EB: cacisa i-DNA kwi-membrane yekholomu yokucoca.
Ikholomu ye-DNA-Kuphela: I-adsorption ethile yeeqhekeza ze-DNA kwijeli.
Amacandelo ekhithi
| Isithinteli GE* |
| Isithinteli WB1 |
| Isithinteli EB |
| Ikholamu yeDNA-Kuphela |
| Imiyalelo |
Iimpawu&izinto eziluncedo
-Uluhlu olubanzi lokubuyiswa kwe-DNA: Iziqwenga ze-DNA zifutshane njenge-30bp kwaye ezinkulu njenge-10kb zinokufunyanwa.
-Ukuphumelela okuphezulu kokubuyisela: Ukusebenza okuphezulu kokubuyisela kunokufikelela ngaphezu kwe-80%.
-Small inkqubo elution: ubuncinane 30μl elution isisombululo ingasetyenziselwa elution, nto leyo enokwandisa ngempumelelo uxinaniso amaqhekeza wachacha DNA.
-Isantya esikhawulezayo: Kulula ukusebenza, ukubuyiswa kweqhekeza le-DNA kunokugqitywa kwimizuzu eyi-15.
-Ukhuseleko: Akukho kukhutshwa kwe-organic reagent efunekayo.
-Umgangatho ophezulu: Iziqwenga ze-DNA ezifunyenweyo zicocekile kakhulu, ezinokuhlangabezana neemvavanyo ezahlukeneyo ezilandelayo.
Isicelo seKit
Le khithi ikulungele ukubuyisela amaqhekeza e-DNA (30bp-10kb) kwiijeli ze-agarose.
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
Le khithi ingagcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo kwiqondo lokushisa (15-25 ° C), ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8 ° C.
Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yitshise kwindawo yokuhlambela amanzi angama-37 ° C imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.
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