Amanzi I-DNA Isolation Kit DNA Extraction and Purification Kit for Water
Inkcazelo yeKit:
Ngokukhawuleza uhlambulule i-DNA ye-genomic ephezulu evela kwi-microorganisms kwiisampuli zamanzi ezahlukeneyo.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
Iinkcukacha
50 Preps
-Lysozyme: i-enzymatic hydrolyze udonga lweseli yeebhaktheriya ezilungileyo.
-Buffer TE: isetyenziselwa ukulungiselela i-100mg / ml isisombululo seLysozyme kunye nokubonelela nge-lysozyme enzymatic hydrolysis environment.
-I-Buffer SG1 & Buffer SG2: Ukubonelela ngemeko yokwetyisa isampula yeprotease.
-I-Forgene Protease: Iisampulu ze-Enzymatic hydrolyze kwindawo ye-protease enzymatic ukukhulula i-genomic DNA.
-I-Buffer SG3: Yenza i-Foregene Protease isebenze kwaye unikeze indawo yokulayisha i-DNA.
-I-Buffer SG4: Inkxaso yokubonelela ngendalo yokulayisha iDNA.
-Buffer PW: Susa ukungcola okufana neprotheni kunye ne-RNA kwiDNA.
-Buffer WB: Susa iiyoni zetyuwa ezishiyekileyo kwiDNA.
-Buffer EB: elute i-DNA kwi-membrane yekholomu yokucoca.
-Ikholamu ye-DNA kuphela: i-adsorb ngokukodwa i-DNA ye-genomic kwi-lysate.
Amacandelo ekhithi
| Isithinteli SG1 |
| Isithinteli SG2 |
| Isithinteli SG3 |
| Isithinteli SG4 |
| Isithinteli PW |
| Buffer WB |
| Isithinteli EB |
| Isithinteli TE |
| I-Foregene Protease |
| I-Lysozyme |
| Ikholamu yeDNA-Kuphela |
| Imiyalelo |
Iimpawu&izinto eziluncedo
-Akukho ungcoliseko lwe-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-DNA ye-genomic ngaphandle kwe-RNase eyongezelelweyo ngexesha lovavanyo, ukuphepha i-laboratory ukuba ingangcoliswa yi-RNase yangaphandle.
-Isantya esikhawulezayo: Umsebenzi ulula, kwaye umsebenzi we-genomic DNA extraction wamanzi ungagqitywa kwimizuzu engama-40.
-Elula: I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ye-centrifugation ephantsi okanye i-ethanol precipitation ye-DNA.
-Ukhuseleko: akukho mfuneko yokutsalwa kwe-organic reagent.
-Umgangatho ophezulu: Iziqwenga ze-DNA ze-genomic ezikhutshwe zikhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
Isicelo seKit
Ifanelekile ukucocwa kwe-DNA ye-genomic kwezi sampuli zilandelayo: amanzi e-aquaculture echibini, amanzi echibi, amanzi epompo, amanzi echibi, amanzi omlambo, amanzi e-lotus kunye nezinye iisampuli.
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
-Le khithi ingagcinwa kwiinyanga ezili-12 phantsi kweemeko ezomileyo kwindawo yokushisa (15-25 ° C), ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8 ° C.
Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yifudumeze nge-37°C ukuhlamba amanzi imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.
-Isisombululo seForegene Protease sinefomula ekhethekileyo, esebenzayo xa igcinwe kwindawo yokushisa ixesha elide (iinyanga ezi-3);xa igcinwe kwi-4 ℃, umsebenzi wayo kunye nokuzinza kuya kuba ngcono, ngoko kuyacetyiswa ukuba uyigcine kwi-4 ℃, khumbula ukuba ungayigcini -20 ℃.
-Ipowder eyomileyo iLysozyme igcinwe kwi -20 ° C;isisombululo seLysozyme esilungisiweyo sahlulwe sibe ngamacandelo amancinci kwaye sigcinwe kwi -20 ° C.
