China Cell-free DNA Isolation Kit Manufacturer kunye noMboneleli |I-Foregene

Ikhithi yokuzahlula ye-DNA engenaSeli

Iseli-free DNA Isolation Kit Featured Image

Inkcazelo yeKit:

Ukwahlukaniswa kwe-cfDNA kwiisampulu zeplasma

Inombolo yekhathalogu TQ01BT0050, TQ01BT0100

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Inkcazo

I-DNA engenaseseli (cfDNA) liqhekeza le-cfDNA elikhutshwe kwiiseli ukuya egazini elinokuthwala ulwazi lwebhayoloji ukusuka.amathumba, iintsholongwane kunye ne-fetus.Uhlalutyo lwe-cfDNA lubonelela ngeempawu eziphambili zoxilongo, ukufumanisa kunye nokubeka iliso kwinkqubela yesifoumbono.Okwangoku, le teknoloji isetyenziswe ngokubanzi kwiinkalo zokufumanisa ukuguqulwa kwe-tumor, isikhokelo esijoliswe kumachiza kunye ne-prognosis,ukufunyaniswa kwe-pathogen kunye nokuhlolwa kwangaphambi kokubeleka okungathinteliyo.

Umgangatho ophezulu we-nucleic acid sisiqinisekiso sophando lwe-genomics.I-Foregene isebenzisa itekhnoloji yokucoca i-superparamagnetic beadukubonelela abasebenzisi ngesisombululo sokutsalwa kwe-nucleic acid esinovakalelo olufikeleleka lula ukutsalwa kwe-nucleic acid ephantsi.ugxininiso.Njengoko kungqinwe ngamawaka eisampulu zeklinikhi, le mveliso inokukhupha umgangatho ophezulu we-cfDNA kulwelo olungenaseli.njengeplasma entsha okanye enomkhenkce, i-serum, i-pleural fluid, i-ascites kunye nolwelo lwe-cerebrospinal kuphando lwezenzululwazi kunye nokuxilongwa kwe-in-vitro yeklinikhi.sebenzisa.

I-Foregene Cell-free DNA Isolation Kit yenzelwe ukwahlula iDNA ejikelezayo engenaselseli (cfDNA) kwiplasma yomntu, serum, ulwelo lomzimba, okanye iisampulu zomchamo.Ikhithi inokukhawuleza kwaye ikhuphe ngokulula i-cfDNA kumanyathelo amathathu: i-lysis / ukubopha, ukuhlamba kunye nolution.Isebenzisa amaso e-superparamagnetic ayilwe ngokukodwa nge-silica-coated superparamagnetic enenkqubo ekhethekileyo ye-lysis / yokubopha ukubuyisela i-nucleic acids ejikelezayo nge-hydrogen bonding kunye ne-electrostatic force, ngaphandle kweeprotheyini zentengiso kunye nobunye ukungcola.Ifanelekile kwizitishi zokusebenzela ezizihambelayo ezizisebenzelayo ukulungiselela naluphi na ucazululo olusezantsi kusetyenziswa iPCR okanye iNGS.

Umxholo weKit kunye noGcino

Itheyibhile 1 Foregene Cell-free DNA Isolation Kit

Ciintente	Runomdla	Isixa(25 T)	Isixa(50 T)	Isixa(100 T)	Sukunyamezela
Ibhokisi1	Isithinteli AL	42 mLx1 ibhotile	89 mLx1 ibhotile	189 mLx1 ibhotile	1 0- 30°C
	Isithinteli AW1	38 mLx1 ibhotile	83 mLx1 ibhotile	165 mLx1 ibhotile	1 0- 30°C
	Isithinteli AW2	15 mLx1 ibhotile	33 mLx1 ibhotile	66 mLx1 ibhotile	1 0- 30°C
	Uvavanyo	3 mLx1 ibhotile	6mLx1 ibhotile	12 mLx1 ibhotile	1 0- 30°C
Ibhokisi2	Amaso kaMagnetic A	3 .2 mLx1 ibhotile	6 .5 mLx1 ibhotile	13 mLx1 ibhotile	2 - 8 ° C
Ibhokisi2	Iprotheni K	5 mLx1 ibhotile	10 .5 mLx1 ibhotile	21 mLx1 ibhotile	2 - 8 ° C

Amanqaku:

[1] Musa ukuxuba amacandelo kwiibhetshi ezahlukeneyo zeekiti.

[2] Isithinteli i-AL inokwenza imvula, engawuchaphazeliyo umsebenzi wayo.Ukuba imvula iyabonakala, nceda ubeke ibhotile ye-reagent kwibhafu yamanzi eyi-56 °C kwi-1 0 - 20 min de i-precipitate inyibilike.Emva koko xuba kakuhle ngaphambi kokusetyenziswa.

[3] Musa ukumkhenkceza amaso eMagnetic A.

Inkqubo

Iimpawu&izinto eziluncedo

-Ukutsalwa ngokufanelekileyo Isivuno esiphezulu sokukhutshwa kwe-DNA, ukucoceka okulungileyo, ukususwa okusebenzayo kokungcola kunye ne-PCR inhibitors esezantsi;

Ukubandakanywa kweSampuli eFlexibleUkutsalwa ngokulula kwe-cfDNA ukusuka kwi-0.5mL ukuya kwi-4mL iisampulu ezinomthamo we-lution ophantsi njenge-20uL

Iinkqubo ezahlukeneyoUkuqinisekiswa kweesampulu zeklinikhi, eyenzelwe ithumba, usulelo kunye nokuxilongwa kwangaphambili kunye ne-biopsy yolwelo

Inkxaso ebanzi ye-Automated Platform Iyahambelana nenkqubo ye-manual ans automated extraction, inkxaso yeTecan, Hamilton, PE kunye nezinye iindawo zokusebenzela ze-nucleic acid extraction.

Ngaphambili: UmGangatho oLungileyo weSampuli yokuKhupha iReagent yeNucleic Acid Utsalo-Mahala iKit yeVtm Inyathelo elinye leNdlela yoVavanyo lwePCR

Okulandelayo: Ixabiso eliphantsi kakhulu leDnasecure Plant Kit yeDNA Extraction Nucleic Acid Purification

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