Genomic DNA Micro Kit Micro Genomic DNA Mini Kit
Inkcazelo yeKit:
Ngokukhawuleza uhlambulule iisampuli ezincinci ukufumana i-DNA ye-genomic ephezulu.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
Inkcazo
Le khithi isebenzisa i-DNA-Only Column enokuthi ibophe ngokukodwa i-DNA, i-Foregene Protease entsha kraca kunye nenkqubo ye-buffer ekhethekileyo, enokuthi ikhuphe ngokufanelekileyo iisampulu ezahlukeneyo zebhayoloji, ezifana nezixa zegazi, iiseli kunye nezilwanyana phakathi kwemizuzu engama-20-80.Izicubu, i-bloodstains eyomileyo, i-cotton swabs yeklinikhi, i-follicles yeenwele, njl., Zihlanjululwe ukuze zifumane i-DNA ye-genomic ephezulu, ephezulu.
Iinkcukacha
50 Preps
Amacandelo ekhithi
| Isithinteli ST1 |
| Isithinteli ST2* |
| Isithinteli PW* |
| Buffer WB |
| Isithinteli EB |
| I-Foregene Protease |
| Ikholamu yeDNA-Kuphela |
| Imiyalelo |
Iimpawu&izinto eziluncedo
-Akukho ungcoliseko lwe-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela i-laboratory ukuba ingangcoliswa yi-RNase yangaphandle.
-Isantya esikhawulezayo: I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye igaya iisampuli zezicubu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
-Elula: I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ye-centrifugation ephantsi okanye i-ethanol precipitation ye-DNA.
-Ukhuseleko: Akukho kukhutshwa kwe-organic reagent efunekayo.
-Umgangatho ophezulu: I-DNA ye-genomic ekhutshweyo inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
-Inkqubo ye-Micro-elution: Inokunyusa i-concentration ye-DNA ye-genomic, ekulungele ukufunyanwa kwe-downstream okanye uvavanyo.
Isicelo seKit
Ilungele ukuhlanjululwa kwezi sampuli zilandelayo ze-DNA ye-genomic: izicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo, i-fresh or anticoagulated cryopreserved trace trace amounts yegazi elipheleleyo, amabala egazi elomileyo, iiseli ezikhuliswe, izicubu ze-microsection, i-follicles yeenwele, i-mouthwashes, njl.
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
-Le khithi ingagcinwa kwiinyanga ezili-12 phantsi kweemeko ezomileyo kwindawo yokushisa (15-25 ° C);ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8 ° C.
Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yitshise kwindawo yokuhlambela amanzi angama-37 ° C imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.
-Isisombululo seForegene Protease sinefomula ekhethekileyo, esebenzayo xa igcinwe kwindawo yokushisa ixesha elide (iinyanga ezi-3);umsebenzi wayo kunye nokuzinza kuya kuba ngcono xa igcinwe kwi-4°C, ngoko kuyacetyiswa ukuba ugcinwe kuyo4°C, khumbula ukuba ungayigcini kwi--20°C.
