Umsila weMouse Direct PCR Kit Direct PCR Lysis Reagent(Umsila weMouse)(yeGenotyping)
Inkcazelo yeKit:
Sebenzisa ngokuthe ngqo umsila wemouse i-lysate njengethempleyithi yokukhulisa i-PCR, ngaphandle kokutsalwa okwahlukileyo kwe-DNA, ngokukhawuleza kunye novakalelo oluphezulu.
◮Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.
◮Imfuno yesampulu incinci, thatha nje imbewu enye.
◮Akukho nyango lukhethekileyo olunjengokusila kunye nokutyumza olufunekayo, kwaye umsebenzi ulula.
◮Inkqubo ye-PCR elungiselelweyo yenza ukuba i-PCR ibe nokucaciswa okuphezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.
Iinkcazelo
Le khithi isebenzisa inkqubo ye-lysis buffer ekhethekileyo ukukhulula ngokukhawuleza i-DNA ye-genomic ukusuka kwimisila yegundane, iindlebe, izihlunu kunye nezinye iisampuli ze-tissue ze-PCR reactions, ngoko ke ifaneleka ngakumbi kuvavanyo olukhulu lwemfuza.
Inkqubo yokukhulula i-DNA ye-genomic kwi-lysis buffer igqityiwe ngaphakathi kwe-10-30 imizuzu kwi-65 ° C.Azikho ezinye iinkqubo ezifana neprotheyini kunye nokususwa kwe-RNA ezifunekayo, kwaye umkhondo okhutshiweyo we-DNA ungasetyenziswa njenge template ye-PCR reaction.
2 × M1-PCR EasyTMUmxube unonyamezelo oluqinileyo kwi-PCR reaction inhibitors, kwaye unokusebenzisa i-lysate yesampuli ukuba ivavanywe njengethemplate yokukhulisa ngokufanelekileyo kunye nokucacileyo.Le reagent iqulethe Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, i-reaction buffer, i-PCR optimizer kunye ne-stabilizer.
I-D-Taq DNA polymerase yipolymerase ye-DNA ephuhliswe ngokukodwa yi-Foregene ukwenzela ukuphendula ngokuthe ngqo kwe-PCR.I-D-Taq DNA polymerase inonyamezelo oluqinileyo kwiintlobo ezahlukeneyo ze-PCR reaction inhibitors, kwaye inokwandisa ngokufanelekileyo izixa ze-DNA kwiinkqubo ezahlukeneyo zokusabela ezintsonkothileyo, kunye nesantya sokukhulisa sinokufikelela kwi-2Kb / min, efanelekileyo kakhulu kwi-Direct PCR reaction.
Iinkcukacha
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Amacandelo ekhithi
| Icandelo I | Buffer MP |
| Foregene Protease Plus | |
| I-6 × i-DNA yokulayisha i-Buffer | |
| Icandelo II | 2 × M1 PCR EasyTMXuba |
| Imiyalelo | |
Iimpawu&izinto eziluncedo
■Akukho mfuneko yokucocwa kwe-DNA okuchitha ixesha kunye nexabiso eliphezulu.
∎ Imfuno yesampulu incinci, kancinane njenge-5mg yenyama yempuku okanye i-2-5mm yomsila wempuku inokuvavanywa.
■ Akukho nyango lukhethekileyo olunjengokusila nokutyumza olufunekayo, kwaye utyando lulula.
■ Inkqubo ye-PCR ephuculweyo yenza i-PCR ibenento ethile ephezulu kunye nokunyamezelana okunamandla kwi-PCR reaction inhibitors.
Iiparamitha zeKit
Isicelo: Umsila wempuku, iindlebe zempuku, izihlunu kunye nezinye izihlunu.
I-DNA ekhutshwe kwisampuli ye-lysis: isetyenziswe kuphela njenge-template ye-PCR.
Ikiti ingasetyenziselwa ezi njongo zilandelayo: ukuchongwa kwe-transgenosis, i-genotyping yezilwanyana, njl.
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
Inxalenye I yale khithi kufuneka igcinwe kwi-2-8℃.
I-reagent Buffer AL inokugcinwa iinyanga ezili-12 phantsi kweemeko ezomileyo;inokugcinwa kwi-2-8℃yokugcina ixesha elide.
I-Reagent Foregene Protease inefomula eyodwa.Ukuqinisekisa umsebenzi wayo kunye nokuzinza, nceda uyigcine ku-4℃iinyanga ezili-12.
I-Reagent 6×I-DNA Loading Buffer inokugcinwa kwi-4℃okanye -20℃ixesha elide.
Inxalenye II yale khithi kufuneka igcinwe kwi -20℃.
I-Reagent 2×M1 PCR LulaTMXuba, ukuba isetyenziswa rhoqo, inokugcinwa kwi-4℃ukugcinwa kwexesha elifutshane (sebenzisa phezulu kwiintsuku ezili-10).
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