• facebook
  • linkedin
  • youtube
English
iphepha_ibhena

I-Foregene DNA Identification System 38Y-C (Case)

I-Foregene DNA Identification System 38Y-C (Case) Umfanekiso okhoyo
  • I-Foregene DNA Identification System 38Y-C (Case)
  • I-Foregene DNA Identification System 38Y-C (Case)
  • I-Foregene DNA Identification System 38Y-C (Case)
  • I-Foregene DNA Identification System 38Y-C (Case)

Inkcazelo yeKit:

I-Foregene Inkqubo yokuchonga iDNA 38Y-UC usebenzisa itekhnoloji yokuleyibheli yemibala emithandathu yefluorescent ukukhulisa 38 Y chromosome STR loci , 3 Y-Indel locikwayeezimbini zolawulo lomgangatho wangaphakathi loci ngexesha elinyeukukhulisa Iisampulu zeCase ezithathwe kwiitemplates zeDNA ecocekileyo.

amandla angaphambili


Khuphela njengePDF

Iinkcukacha zeMveliso

Iithegi zeMveliso

Inkcazo

I-Foregene Inkqubo yokuchonga iDNA 38Y-UC usebenzisa itekhnoloji yokuleyibheli yemibala emithandathu yefluorescent ukukhulisa 38 Y chromosome STR loci , 3 Y-Indel locikwayeezimbini zolawulo lomgangatho wangaphakathi loci ngexesha elinyeukukhulisa Iisampulu zeCase ezithathwe kwiitemplates zeDNA ecocekileyo.

Umxholo weKit

Ukulungiswa kwenkqubo yokwandisa

Iicompositio yokulungiselelan of Umgangathoutsalokunye nenkqubo yokwandisa

 Master Mix

Cabachasi

10μl inkqubo (μl)

2.5×PCR Master Mix C I

4.0

5 x 38Y-C Primer Mix

2.0

itemplate yeDNA

1- 4

Amanzi adibeneyo

Gcwalisa ukuya kumthamo wokusabela we10 μl

Uhlalutyo lwedatha

Igrafu ehlonyelweyo 1:Imephu yeAllelic Ladder Typing

Igrafu ehlonyelweyo 2:DNA ukuchwetheza umgangatho 9948 wokuchwethezaimephu

Ugcino lweReagent

1. Nceda uzigcine ngaphantsi kwe-20°C (ukuba azisetyenziswanga okwethutyana), emva kokufumana iikhithi ezikhenkcezisiweyo kumkhenkce owomileyo okanye iipakethi zejeli zomkhenkce.

2. Nceda usore icandelo langaphambili le-PCR master mix liku--20 ° C, emva kokuba ikhithi ikhutshelwe ukusetyenziswa kwaye i-reagents eseleyo yangaphambili igcinwa kwi-4 ° C ukuphepha ukukhenkceza okuphindaphindiweyo kunye nokunyibilika.Ukuba idosi enye incinci, kuyacetyiswa ukuba igcinwe kwi -20 ° C emva kwe-aliquoting.

3. Gcina amacandelo emva kokuphendula kwi-4 ° C, gwema ukukhenkceza ngokuphindaphindiweyo kunye nokuqhaqha, kwaye ungachukumisi ama-reagents ngaphambi kokuphendula ukuphepha ukungcoliswa.

  • Ngaphambili:
  • Okulandelayo:
    • Bhala umyalezo wakho apha kwaye uwuthumele kuthi

    EzinxulumeneyoIimveliso

    Cofa u-enter ukukhangela okanye i-ESC ukuvala