I-Foregene DNA Identification System 25A-C (Case)
Inkcazelo yeKit:
I-Foregene DNA Identification System 25A-C isebenzisa iteknoloji yokubhalwa kwe-fluorescence enemibala emithandathu ukukhulisa i-23 autosomal STR loci, enye i-Amel loci, enye i-Yindel loci kunye ne-loci ezimbini zokulawula umgangatho wangaphakathi ngexesha lokukhulisa i-templates ye-DNA ekhutshwe kwaye ihlanjululwe kwiisampuli zecala.
Inkcazo
I-Foregene DNA Identification System 25A-C isebenzisa iteknoloji yokubhalwa kwe-fluorescence enemibala emithandathu ukukhulisa i-23 autosomal STR loci, enye i-Amel loci, enye i-Yindel loci kunye ne-loci ezimbini zokulawula umgangatho wangaphakathi ngexesha lokukhulisa i-templates ye-DNA ekhutshwe kwaye ihlanjululwe kwiisampuli zecala.
Umxholo weKit
Ukulungiswa kwenkqubo yokwandisa
ULungiselelo loNxibelelwano loMgangatho wokutsalwa kunye nenkqubo yokukhulisa
| Amacandelo | 10μl inkqubo(μl) | |
| Master Mix | 2.5×PCR Premix C Ⅰ | 4.0 |
| 10 × 25A-CPrimer Mix | 1.0 | |
| itemplate yeDNA | 1—5 | |
| Amanzi adibeneyo | Yenza umthamo wokusabela we-10μl |
Uhlalutyo lwedatha
Igrafu ehlonyelweyo 1:Ileli genotyping of allele
Igrafu ehlonyelweyo 2:DNA ukuchwetheza umgangatho 9948 wokuchwethezaimephu
Ugcino lweReagent
1. Emva kokufumana ikiti, nceda uyigcine ngaphantsi kwe-20℃ ukuba awuyisebenzisi okwangoku.
2. Emva kokuba ikhithi ikhutshiwe kwaye isetyenziswe, i-pre-reaction component PCR premixed solution igcinwa kwi -20℃, kwaye i-reagents eseleyo yangaphambili igcinwe kwi-4℃ ukuphepha ukunyibilika okuphindaphindiweyo komkhenkce.Ukuba idosi enye incinci, kuyacetyiswa ukuba igcinwe kwi -20 ° C emva kokupakishwa.
3. Emva kokusabela, amacandelo agcinwa kwi-4℃ ukunqanda ukunyibilika okuphindaphindiweyo.Ungazichukumisi ii-reagents zangaphambili ukunqanda ungcoliseko.
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