I-Foregene DNA Identification System 30A (Ayikhutshiweyo)
Inkcazelo yeKit:
Inkqubo yokuchonga ye-ForEGENE DNA 30A isebenzisa ubuchwepheshe bokulebula ngemibala emithandathu ye-fluorescent ukuzekhulisa 29autosomalSIndawo yeTR, NyeI-Amel locus kunye ne-Yindel locus enye ngexesha elinye, kwaye inokukhulisa ngokuthe ngqo iphepha lokucoca, i-FTAamakhadi, iintambo zomqhaphu, amakhadi amathe,kunye ne-mswab ngaphandle.
Inkcazo
Inkqubo yokuchonga ye-ForEGENE DNA 30A isebenzisa ubuchwepheshe bokulebula ngemibala emithandathu ye-fluorescent ukuzekhulisa 29autosomalSIndawo yeTR, NyeI-Amel locus kunye ne-Yindel locus enye ngexesha elinye, kwaye inokukhulisa ngokuthe ngqo iphepha lokucoca, i-FTAamakhadi, iintambo zomqhaphu, amakhadi amathe,kunye ne-mswab ngaphandle.
Umxholo weKit
ULungiselelo lweNkqubo yoKwandiswa
Itheyibhile 1: Ukuqulunqwa kokwakhiwa koMgangatho (okungatsalwanga) ukusabela kokukhulisa
|
| Cabachasi | 25 Inkqubo ye-μl (μl) | 10 Inkqubo ye-μl (μl) |
| Master Mix | 4 × Master Mix VII | 6.25 | 2.5 |
| 5 x 30AI-Primer Mix | 5.0 | 2 | |
| Amanzi adibeneyo | 13.75 | 5.5 | |
| Bimpahla ibala | Ububanzi 1.2mm | Ububanzi 1.2mm | |
| Ti-otal reaction volume | 25 μl | 10 μl | |
Uhlalutyo lwedatha
Igrafu ehlonyelweyo 1:Imephu yeAllelic Ladder Typing
Igrafu ehlonyelweyo 2:DNA ukuchwetheza umgangatho 9948 wokuchwethezaimephu
Ugcino lweReagent
1. Nceda ugcine ngaphantsi kwe-20°C, emva kokufumana iikhithi ezikhenkcezisiweyo kumkhenkce owomileyo okanye iipakethi zejeli zomkhenkce.
2. Nceda ugcine i-pre-reaction component 4 × premix VII kwi--20 ° C, emva kokuba ikiti ikhutshwe ukuze isetyenziswe, kwaye ugcine ezinye ii-reagents ezisele zangaphambili kwi-4 ° C ukuphepha ukukhenkceza okuphindaphindiweyo kunye nokunyibilika.Ukuba idosi enye incinci, kuyacetyiswa ukuba igcinwe kwi -20 ° C emva kokucaphula,
3. Gcina amacandelo emva kokuphendula kwi-4 ° C, gwema ukukhenkceza ngokuphindaphindiweyo kunye nokuqhaqha, kwaye ungachukumisi ama-reagents ngaphambi kokuphendula ukuphepha ukungcoliswa.
