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I-Foregene DNA Identification System 30 AC (Case)

I-Foregene DNA Identification System 30 AC (Case) Umfanekiso okhoyo
  • I-Foregene DNA Identification System 30 AC (Case)
  • I-Foregene DNA Identification System 30 AC (Case)
  • I-Foregene DNA Identification System 30 AC (Case)
  • I-Foregene DNA Identification System 30 AC (Case)

Inkcazelo yeKit:

I-Foregene Inkqubo yokuchonga iDNA30A-UC usebenzisa iteknoloji yokuleyibhela ngemibala emithandathu yefluorescent ukuzekhulisa 29autosomalSTR indawo, 1 I-Amel locus, i-1 Yindellocus, kwaye1 Iisayithi zeSexual ezikhethekileyo, 2iisayithi zolawulo lomgangatho wangaphakathi, ezisetyenziselwa ukukhulisa iDNAitemplate ekhutshiweyo kwaye yahlanjululwa kuyoCase izixhobo.

amandla angaphambili


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Inkcazo

I-Foregene Inkqubo yokuchonga iDNA30A-UC usebenzisa iteknoloji yokuleyibhela ngemibala emithandathu yefluorescent ukuzekhulisa 29autosomalSTR indawo, 1 I-Amel locus, i-1 Yindellocus, kwaye1 Iisayithi zeSexual ezikhethekileyo, 2iisayithi zolawulo lomgangatho wangaphakathi, ezisetyenziselwa ukukhulisa iDNAitemplate ekhutshiweyo kwaye yahlanjululwa kuyoCase izixhobo.

Umxholo weKit

Ukulungiswa kwenkqubo yokwandisa

Itheyibhile 2: ULungiselelo lokuQukwa kweSistim yoMgangatho wokutsalwa kunye nokukhulisa

 

Cabachasi

Inkqubo ye-25ul (µl)

10μl inkqubo (μl)

Master Mix

 2.5× PremixCⅡ

10.0

4.0
5 x 30 I-AC Primer Mix

5.0

2.0
itemplate yeDNA

2.5-10

1-4
Amanzi adibeneyo

Yenza umthamo wokuphendula ube ngama-25μl

Yenza umthamo wokusabela we10 μl

Uhlalutyo lwedatha

Igrafu ehlonyelweyo 1:Imephu yeAllelic Ladder Typing

Igrafu ehlonyelweyo 2:DNA ukuchwetheza umgangatho 9948 wokuchwethezaimephu

Ugcino lweReagent

1. Nceda ugcine ngaphantsi kwe-20°C, emva kokufumana iikhithi ezikhenkcezisiweyo kumkhenkce owomileyo okanye iipakethi zejeli zomkhenkce.

2. Nceda ugcine icandelo le-pre-reaction component 2.5 × premix CII kwi -20 ° C emva kokuba ikiti ikhutshwe ukuze isetyenziswe, kwaye i-reagents esele yangaphambili igcinwe kwi-4 ° C ukuphepha ukukhenkceza ngokuphindaphindiweyo kunye nokunyibilika.Ukuba idosi enye incinci, kuyacetyiswa ukuba igcinwe kwi -20 ° C emva kwe-aliquoting.

3. Gcina amacandelo emva kokuphendula kwi-4 ° C, gwema ukukhenkceza ngokuphindaphindiweyo kunye nokuqhaqha, kwaye ungachukumisi ama-reagents ngaphambi kokuphendula ukuphepha ukungcoliswa.

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