I-Foregene DNA yokuchongwa kweNkqubo ye-Y Plus (Engatsaliyo)
Inkcazelo yeKit:
I-ForegeneInkqubo yokuchonga i-DNA iY Plus isebenzisa ubuchwephesha bokulebula ngemibala emithandathu ye-fluorescent ukukhulisa i-20 Y chromosome core loci, 15 loci ekhethwayo, 6 enye indawo loci kunye 3 Y indel loci ngexesha elinye..
Inkcazo
I-ForegeneInkqubo yokuchonga i-DNA iY Plus isebenzisa ubuchwephesha bokulebula ngemibala emithandathu ye-fluorescent ukukhulisa i-20 Y chromosome core loci, 15 loci ekhethwayo, 6 enye indawo loci kunye 3 Y indel loci ngexesha elinye..
Umxholo weKit
Ukulungiswa kwenkqubo yokwandisa
Iukwakheka kwamalungiselelokwesumgangatho(ngaphandle kokutsalwa)inkqubo yokwandisa
| Cabachasi | 25 μlinkqubo (μl) | 10μl inkqubo (μl) | |
| Master Mix | 4 × Master MixVII | 6.25 | 2.5 |
| 5×Y Plus Primer Mix | 5.0 | 2.0 | |
| Amanzi adibeneyo | 13.75 | 5.5 | |
| Bimpahla ibala | Ububanzi 1.2mm | Ububanzi 1.2mm | |
| Ti-otal reaction volume | 25 μl | 10 μl | |
Uhlalutyo lwedatha
Igrafu ehlonyelweyo 1:Imephu yeAllelic Ladder Typing
Igrafu ehlonyelweyo 2:DNA ukuchwetheza umgangatho 9948 wokuchwethezaimephu
Ugcino lweReagent
1. Nceda ugcine ngaphantsi kwe-20°C(Ukuba ayisetyenziswanga okwethutyana),emva kokufumana iikhithi ezikhenkcezisiweyo kumkhenkce owomileyo okanye iipakethe zejeli zomkhenkce.
2. Nceda ugcine i-pre-reaction component 4 × premix VII kwi--20 ° C, emva kokuba ikiti ikhutshelwe ukusetyenziswa, kunye nezinye ii-reagents ezisele zangaphambili zigcinwe kwi-4 ° C ukuphepha ukukhenkceza okuphindaphindiweyo kunye nokunyibilika.Ukuba idosi enye incinci, kuyacetyiswa ukuba igcinwe kwi -20 ° C emva kwe-aliquoting.
3. Gcina amacandelo emva kokuphendula kwi-4 ° C, gwema ukukhenkceza ngokuphindaphindiweyo kunye nokuqhaqha, kwaye ungachukumisi ama-reagents ngaphambi kokuphendula ukuphepha ukungcoliswa.
-
I-Foregene DNA Identification System 30 AC (Case)
-
I-Foregene DNA Identification System 20A (I-DN yasimahla...
-
Inkqubo yokuchonga ye-Foregene DNA 27Y
-
I-Foregene DNA Identification System 25A-C (Case)
-
I-Foregene DNA Identification System 38Y-C (Case)
-
I-Foregene DNA Identification System 30A (Ayiyo Ext...
