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Inkqubo yokuchonga ye-Foregene DNA 27Y

I-Foregene DNA yokuchongwa kweNkqubo ye-27Y Umfanekiso obonakalayo
  • Inkqubo yokuchonga ye-Foregene DNA 27Y
  • Inkqubo yokuchonga ye-Foregene DNA 27Y
  • Inkqubo yokuchonga ye-Foregene DNA 27Y

Inkcazelo yeKit:

I-Foregene I-DNA Identification System 27Y isebenzisa ubuchwepheshe bokulebula ngemibala emithandathu ye-fluorescent ukukhulisa i-27 Y-STR loci ngexesha elinye.Kubandakanywa: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS3, DY39, DY3F38, DY398 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.

Ikiti inamandla okuthintela ukunqanda, kwaye inokukhulisa ngokuthe ngqo iphepha lokucoca, ikhadi le-FTA, i-cotton swab, i-saliva card, i-oral swab, kwaye ingasetyenziselwa ukukhupha kunye nokucoca i-templates ye-DNA kwizinto zecala.

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Inkcazo

I-Foregene I-DNA Identification System 27Y isebenzisa ubuchwepheshe bokulebula ngemibala emithandathu ye-fluorescent ukukhulisa i-27 Y-STR loci ngexesha elinye.Kubandakanywa: DYS456, YGATAH4, DYS439, DYS19, DYS392, DYS576, DYS627, DYS391, DYS437, DYS570, DYS635, DYS448, DYS533, DYS3, DY39, DY3F38, DY398 0 , DYS389II, DYS438, DYS518, DYS460, DYS458, DYS481, DYS385, DYS449.

Ikiti inamandla okuthintela ukunqanda, kwaye inokukhulisa ngokuthe ngqo iphepha lokucoca, ikhadi le-FTA, i-cotton swab, i-saliva card, i-oral swab, kwaye ingasetyenziselwa ukukhupha kunye nokucoca i-templates ye-DNA kwizinto zecala.

Umxholo weKit

Ikhithi yovavanyo lwe-reagent

Igama lelungu

Umthamo (μl/ityhubhu)

Ubungakanani (ityhubhu)

PCR ikhithi yokusabela kwangaphambili

 I-2 × PCR Reaction Master Mix

1250

2
5 x 27Y Primer Mix

500

2
DNA Typing Standard 9948

20

2
Amanzi adibeneyo

1700

2

I-Post-PCR Reaction Kit

 27Y allele umxube Ileli

20

2
Orenji ubunzima molekyuli umgangatho wangaphakathi ORG500

150

2

Uhlalutyo lwedatha

1. Vula i-software ye-ID ye-Gene Mapper.When using this kit for the first time, you need to import panels, bin set, corresponding analysis method, and size standard ( ORG500 : 65, 70, 80, 100, 120 , 140, 160, 180, 200, 225, 250, 275, 300, 330, 360, 390, 420, 450, 490, 500).

2. Ngenisa idatha ye-electrophoresis, khetha iiparitha zokuhlalutya ezihambelanayo ezifana nephaneli, indlela yokuhlalutya, kunye nomgangatho wesayizi, kwaye utshintshe uhlobo lwesampula ye-Ladder kwi-"Allelic Ladder" kwikholamu ye "Sample Type";qala ukuhlalutya idatha.

Ugcino lweReagent

1. Nceda uzigcine ngaphantsi kwe-20 ° C (ukuba zingasetyenziswanga okwethutyana), emva kokufumana iikhithi ezikhenkcezisiweyo kumkhenkce owomileyo okanye i-gel ice packs.

2. Nceda ugcine ikiti kwi-4 ° C ngaphambi kokuphendula ukuphepha ukukhenkceza okuphindaphindiweyo kunye nokunyibilika, emva kokuba ikiti ikhutshelwe ukusetyenziswa;i-enzyme kufuneka igcinwe kwi-20°C.

2. Emva kokusabela, ikiti (kubandakanywa nomgangatho wangaphakathi we-molecular kunye neleli) kufuneka igcinwe "kwigumbi lokubona i-electrophoresis" ngo-4 ° C, ugweme ukukhenkceza okuphindaphindiweyo kunye nokunyibilika, kwaye ugweme ukudibanisa nekhithi yokusabela kwangaphambili ukuze ugweme ukungcoliswa.

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