I-Foregene DNA Identification System 20A (I-DNA yasimahla yokutsalwa)
Inkcazelo yeKit:
Inkqubo yokuchonga i-Foregene DNA i-20A isebenzisa iteknoloji yokubhalwa kwe-fluorescent yemibala emihlanu ukuze ikhulise ngaxeshanye i-19 STR loci kunye ne-1 yesini se-loci kwi-tube enye.Ngelixa igubungela i-13 CODIS core loci, i-20 core loci ihlangabezana neemfuno zoMphathiswa woKhuseleko loLuntu kwikhithi ye-autosomal STR.Uyindoda esekunene yolwakhiwo lwedatha yedatha ye-DNA kunye nokuchongwa kobudlelwane benkundla.
Inkcazo
Inkqubo yokuchonga i-Foregene DNA i-20A isebenzisa iteknoloji yokubhalwa kwe-fluorescent yemibala emihlanu ukuze ikhulise ngaxeshanye i-19 STR loci kunye ne-1 yesini se-loci kwi-tube enye.Ngelixa igubungela i-13 CODIS core loci, i-20 core loci ihlangabezana neemfuno zoMphathiswa woKhuseleko loLuntu kwikhithi ye-autosomal STR.Uyindoda esekunene yolwakhiwo lwedatha yedatha ye-DNA kunye nokuchongwa kobudlelwane benkundla.
Umxholo weKit
| iKit Componet | Igama leComponent | Umthamo (μl / tube) | Ubungakanani (ityhubhu) |
| I-PCR yokusabela kwangaphambili | 2.5×PCR reaction buffer Ⅲ | 1000 | 2 |
| 5 × 20A primer mix | 500 | 2 | |
| Lawula iDNA 9947A(1ng/μl) | 25 | 1 | |
| Amanzi adibeneyo | 1700 | 2 | |
| Taq Polymerase | I-Taq polymerase Ⅲ | 40 | 2 |
| I-PCR Reaction reagent | Ileli yeAllelic20A | 40 | 1 |
| Ubunzima bemolekyuli yangaphakathi standardORG 500 | 150 | 2 | |
| Phawula | |||
Uhlalutyo lwedatha
1. Vula i-software ye-ID ye-Genemapper.Xa usebenzisa le khit okwesihlandlo sokuqala, kufuneka ungenise iiphaneli, i-bin iseti, indlela yokuhlaziya ehambelana nayo, kunye nobungakanani beMigangatho (i-Arg500: 65, 420, 49, 490, 590, 59, 59, 59, 59, 59, 59, 59, 59, 59, 59, 59, 59, 59, 59, 59, 59
2. Ngenisa idatha ye-electrophoresis, khetha iiparitha zokuhlalutya ezihambelanayo ezifana nephaneli, indlela yokuhlalutya, kunye nomgangatho wesayizi, kwaye utshintshe uhlobo lwesampula ye-Ladder kwi-"Allelic Ladder" kwikholamu ye "Sample Type";qala ukuhlalutya idatha.
Ugcino lweReagent
1. Emva kokufumana ikiti efriziwe kwiqhwa elomileyo okanye iipakethi zeplastiki zeqhwa, ukuba zingasetyenziswanga okwethutyana, nceda uyigcine ngaphantsi kwe-20 ° C ixesha elide.
2. Emva kokuba ikiti ikhutshiwe kwaye isetyenziswe, ikiti kufuneka igcinwe kwi-4 ° C ngaphambi kokusabela ukuphepha ukukhenkceza okuphindaphindiweyo kunye nokunyibilika;I-Taq enzyme kufuneka igcinwe kwi -20°C.
3. Emva kokuphendula, ikiti (kubandakanywa nomgangatho wangaphakathi we-molecular kunye neleli) kufuneka igcinwe "kwigumbi lokubona i-electrophoresis" ngo-4 ° C, ugweme ukukhenkceza okuphindaphindiweyo kunye nokunyibilika, kwaye ugweme ukudibanisa nekhithi yokusabela kwangaphambili ukuze ugweme ukungcoliswa.
