I-Blood SuperDirectᵀᴹ PCR Kit(UNG)-Heparin Blood Direct PCR Master Mix for Genotyping of Blood
Inkcazelo yeKit:
Sebenzisa ngokuthe ngqo i-heparin anticoagulated igazi elipheleleyo njengethempleyithi yokukhulisa i-PCR, enovakalelo oluphezulu kunye nokusebenza kakuhle kokukhulisa.Inkqubo ye-UNG yokuchasana nongcoliseko yongezwe ukukhusela iimpawu zobuxoki.
◮Inkqubo inamandla okukhulisa amandla kwaye inokubona ngovakalelo i-genome kunye namaqhekeza e-DNA ekujoliswe kuwo angaphandle egazini.
◮2 × SuperEasyTMUmxube (UNG) -I-Heparin Ukunyamezela kwegazi eliphezulu: ukuya kwi-45% kwigazi lomntu kunye ne-20% kwigazi le-mouse.
◮Isampulu ivalwe ngokupheleleyo, ngoko ke akukho mfuneko yakuba nexhala malunga nokungcoliseka kwesampulu kunye neziphumo zobuxoki zePCR.Inkqubo yePCR echasene nongcoliseko
◮2 × SuperEasyTMHlanganisa (UNG), ukuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR, kwaye uqinisekise ukuchaneka kunye nokuchaneka kokukhulisa.
Iinkcukacha
200 ×20μl rxns, 2000 ×20μl rxns
2 × SuperEasyTMUmxube (UNG) -Heparin: Ngesiseko se-2 × SuperEasyTMI-Mix-Heparin, i-dUTP isetyenziswe endaweni ye-dTTP, kwaye i-enzyme ye-UNG ekwazi ukuthobisa i-template ye-dUTP yongezwa ngexesha elifanayo, elinokuthintela ngokufanelekileyo ukungcoliswa kweemveliso ze-PCR zokukhulisa.Iqulethe i-D-Taq DNA Polymerase elungiswe ngokukodwa, i-dNTPs, i-MgCl2, i-reaction buffer, i-PCR reaction enhancer, i-optimizer kunye ne-stabilizer, njl. njl2O kwi-2 × i-SuperEasy TM Mix ehambelanayo (UNG) -Heparin ukuba isetyenziswe kwi-PCR reaction.
I-6 × i-DNA yokuLayisha i-Buffer: I-Buffer yokuLayisha ayinayo i-SDS.Kunconywa ukusebenzisa i-6 × DNA Loading Buffer efakwe kunye nekiti xa wenza i-agarose gel electrophoresis ukuze ufumane iziphumo ezilungileyo ze-electrophoresis.Musa ukusebenzisa i-Loading Buffer equkethe i-SDS, ngaphandle koko kuya kubakho umsila omkhulu wokukhanya okukhanyayo kwindlela ngexesha le-electrophoresis, eya kuchaphazela iziphumo zovavanyo.
Amacandelo ekhithi
| 2 × SuperEasyTMUmxube(UNG)-Heparin |
| I-6 × i-DNA yokulayisha i-Buffer |
| Imiyalelo |
Iimpawu&izinto eziluncedo
-Inkqubo inamandla okukhulisa amandla kwaye inokubona ngovakalelo i-genome kunye namaqhekeza e-DNA ekujoliswe kuwo angaphandle egazini.
-2 × SuperEasyTMUmxube (UNG) -I-Heparin Ukunyamezela kwegazi eliphezulu: ukuya kwi-45% kwigazi lomntu kunye ne-20% kwigazi le-mouse.
-Isampuli ivalwe ngokupheleleyo, ngoko akukho mfuneko yakukhathazeka malunga nongcoliseko lwesampulu kunye neziphumo zobuxoki ze-PCR.Inkqubo yePCR yokuchasana nongcoliseko 2× SuperEasyTMHlanganisa (UNG), ukuphelisa ngokufanelekileyo ukungcola okubangelwa yimveliso ye-PCR, kwaye uqinisekise ukuchaneka kunye nokuchaneka kokukhulisa.
Isicelo seKit
Inikezelwe ukuchongwa ngqo kwePCR ye-Heparin anticoagulated lonke igazi.(Kubandakanya: igazi lomntu, igazi lempuku, igazi lenkukhu, igazi lentaka, igazi lenkomo, igazi lenja, njl.)
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
Yonke inkqubo yokuthutha i-ice box yobushushu obuphantsi, ukuqinisekisa ukuba ikiti ikwimo ye-<4 ℃.
2 × SuperEasyTMI-Mix (UNG) -I-Heparin, ukuba isetyenziswe rhoqo, ingaphinda igcinwe kwi-4 ° C yokugcina ixesha elifutshane (isetyenziswe kwiintsuku ze-10).
I-6 × DNA Loading Buffer, inokugcinwa kwi-4 ° C okanye -20 ° C ixesha elide.
