I-Animal miRNA Isolation Kit Ukutsalwa kweMicroRNA kunye neeKits zokuCoca kwizilwanyana
Inkcazelo yeKit:
Ngokukhawuleza nangokufanelekileyo ukukhupha amaqhekeza amancinci e-RNA ye-20-200nt miRNA, i-siRNA, i-snRNA kwizicubu ezahlukeneyo zezilwanyana kunye neeseli.
◆Yonke le nkqubo iqhutywa kubushushu begumbi (15-25°C), ngaphandle kokuhlamba umkhenkce kunye ne-centrifugation yobushushu obuphantsi.
◆Isethi epheleleyo ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
◆Ikholomu yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukwenzela ukuba ikiti inokususa ukungcola kwe-DNA ye-genomic ngaphandle kokongeza i-DNase eyongezelelweyo.
◆Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
◆Isantya esikhawulezayo: Umsebenzi ulula kwaye unokugqitywa kwimizuzu engama-30.
◆Ukhuseleko: akukho kukhutshwa kwe-organic reagent efunekayo.
◆Umgangatho ophezulu: Iziqwenga ze-RNA ezikhutshiweyo zicocekile kakhulu, azinaprotheyini kunye nolunye ukungcola, kwaye zinokuhlangabezana neemfuno zomlambo osezantsi.
◆izicelo ezahlukeneyo zovavanyo
Inkcazo
Le khithi isebenzisa ikholamu ye-spin kunye nefomula ephuhliswe yinkampani yethu, enokuthi ikhuphe ngokufanelekileyo amaqhekeza amancinci e-RNA anje nge-20-200nt miRNA, siRNA, snRNA ukusuka kwizicubu ezahlukeneyo zezilwanyana kunye neeseli.Ikiti inikezela i-DNA-Cleaning Column, enokuthi ihlukanise ngokulula i-supernatant kwi-lysate ye-tissue kwaye isuse i-DNA kunye ne-RNA ende kwisampuli, elula kwaye igcina ixesha;Ikholomu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA ngefomula ekhethekileyo , Iyakwazi ukucubungula inani elikhulu leesampuli ngexesha elinye.Yonke inkqubo i-RNase-Free, ukuze i-miRNA ehlanjululweyo ingathotywanga;I-Buffer miRW1, i-Buffer miRW2 i-buffer yokuhlamba inkqubo, ukuze i-RNA efunyenweyo ingabinaprotheyini, i-DNA, ii-ion, kunye nongcoliseko lwe-organic compound.
Amacandelo ekhithi
| Animal miRNA Isolation Kit | ||||
| Imixholo yekhithi | RE-0101T | RE-01011 | RE-01012 | RE-01014 |
| 5 T | 50 T | 100T | 200T | |
| Buffer miRL* | 3ml | 25ml | 50ml | 100ml |
| Isithinteli miRW1 | 4ml | 35ml | 70ml | 140ml |
| Buffer miRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-Free ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| IKholam ye-RNA kuphela | 5 iiseti | 50iiseti | 100iiseti | 200iiseti |
| Ikholamu yokucoca iDNA | 5 iiseti | 50iiseti | 100iiseti | 200iiseti |
| IFU | 1 iqhekeza | 1 iqhekeza | 1 iqhekeza | 1 iqhekeza |
Iimpawu&izinto eziluncedo
◆Yonke le nkqubo iqhutywa kubushushu begumbi (15-25°C), ngaphandle kokuhlamba umkhenkce kunye ne-centrifugation yobushushu obuphantsi.
◆Isethi epheleleyo ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
◆Ikholomu yokucoca i-DNA ibophelela ngokukodwa i-DNA, ukwenzela ukuba ikiti inokususa ukungcola kwe-DNA ye-genomic ngaphandle kokongeza i-DNase eyongezelelweyo.
◆Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
◆Isantya esikhawulezayo: Umsebenzi ulula kwaye unokugqitywa kwimizuzu engama-30.
◆Ukhuseleko: akukho kukhutshwa kwe-organic reagent efunekayo.
◆Umgangatho ophezulu: Iziqwenga ze-RNA ezikhutshiweyo zicocekile kakhulu, azinaprotheyini kunye nolunye ukungcola, kwaye zinokuhlangabezana neemfuno zomlambo osezantsi.
◆izicelo ezahlukeneyo zovavanyo
Isicelo seKit
Ilungele ukutsalwa kunye nokucocwa kwe-miRNA kwiintlobo ezahlukeneyo zezicubu zezilwanyana ezitsha okanye ezikhenkcezisiweyo okanye iiseli.
Ugcino kunye nobomi beShelf
Le khithi ingagcinwa iinyanga ezingama-24 phantsi kweemeko ezomileyo kwiqondo lobushushu begumbi (15-25°C), ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kuyo2-8°C.I-Buffer miRL inokubekwa kuyo4°Ckwinyanga ye-1 emva kokongezaβ-mercaptoethanol(kuyacetyiswa ukuba uyifake ngoku).
