I-Soil DNA Isolation Kit yeKhitshi yokuNxila iiKhiti zokuCoca & ii-reagents ze-DNA yomhlaba
Inkcazelo yeKit:
Ngokukhawuleza ucoce kwaye ufumane i-DNA ye-genomic ekumgangatho ophezulu kwiisampulu zesampulu zomhlaba ezahlukeneyo.
◮Akukho susulelo lwe-RNase:IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube nokwenzeka ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
◮Isantya esiphezulu:I-Foregene Protease inomsebenzi ophezulu kuneeproteases ezifanayo, kwaye yetyise iisampulu zethishu ngokukhawuleza;ukusebenza kulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-20-80.
◮Iluncedo:I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4 ° C ephantsi kwe-centrifugation okanye i-ethanol precipitation ye-DNA.
◮Ukhuseleko:Akukho kukhutshwa kwe-organic reagent efunekayo.
◮Umgangatho ophezulu:I-DNA ekhutshiweyo ye-genomic inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
◮Inkqubo yeMicro-elution:Inokunyusa uxinaniso lwe-DNA ye-genomic, elungele ukufunyanwa okanye ukuvavanywa komlambo osezantsi.
Inkcazo
Le khithi ibonelela ngendlela ekhawulezayo nelula yokukhupha i-genomic DNA kwimithombo eyahlukeneyo yomhlaba.Kukho inani elikhulu lee-inhibitors ezifana ne-humic acid kunye neeoni zetsimbi kwiisampulu zomhlaba.Nangona ezi zinto zikhoyo kwiimali ezincinci kwi-DNA ecocekileyo, ziya kuba nefuthe kwiimpendulo ezisezantsi, ezifana ne-PCR kunye nokunciphisa i-enzyme digestion.Ngoko ke, isitshixo sokucoca i-DNA yomhlaba yindlela yokususa ngokufanelekileyo i-inhibitors emhlabeni.
Le kit isebenzisa i-DNA-Only Column enokuthi ibophe ngokukodwa kwi-DNA, i-brand-entsha ye-Foregene Protease kunye nenkqubo ye-buffer ekhethekileyo, enokususa ngokufanelekileyo i-inhibitors ezahlukahlukeneyo emhlabeni ngaphandle kokutsalwa kwe-solvent ye-organic okanye i-ethanol kunye ne-isopropanol precipitation.Ukukhutshwa kwe-DNA kwiisampuli zomhlaba kunokugqitywa kwimizuzu engama-90.
Iinkcukacha
50 Preps, 100 Preps, 250 Preps
Amacandelo ekhithi
| Isithinteli SG1 |
| Isithinteli SG2 |
| Isithinteli SG3 |
| Isithinteli SG4 |
| Isithinteli PW |
| Buffer WB |
| Isithinteli EB |
| Isithinteli TE |
| I-Foregene Protease |
| Ikholamu yeDNA-Kuphela |
| Imiyalelo |
Iimpawu&izinto eziluncedo
-Akukho ungcoliseko lwe-RNase: IKholamu ye-DNA-Kuphela enikezelwe yikiti yenza kube lula ukususa i-RNA kwi-DNA ye-genomic ngaphandle kokongeza i-RNase ngexesha lovavanyo, ukuthintela ilabhorethri ukuba ingangcoliswa yi-RNase yangaphandle.
-Isantya esikhawulezayo: Umsebenzi ulula, kwaye i-genomic DNA extraction operation ingagqitywa kwimizuzu engama-90.
-Elula: I-centrifugation yenziwa kwiqondo lokushisa, kwaye akukho mfuneko ye-4°C iqondo eliphantsi lobushushu becentrifugation okanye i-ethanol precipitation yeDNA.
-Ukhuseleko: Akukho kukhutshwa kwe-organic reagent efunekayo.
-Umgangatho ophezulu: I-DNA ye-genomic ekhutshweyo inamaqhekeza amakhulu, akukho RNA, akukho RNase, kunye nomxholo we-ion ophantsi kakhulu, onokuhlangabezana neemfuno zeemvavanyo ezahlukeneyo.
Isicelo seKit
Ilungele ukucocwa kwe-DNA ye-genomic kwezi sampuli zilandelayo: iisampulu zentlenga, umhlaba weentyatyambo, umhlaba onyathelayo, umhlaba wehlathi, umhlaba oyinkunkuma, umhlaba wedama, umhlaba osecaleni kwechibi, umhlaba onengca kunye nezinye iisampuli.
Ukuhamba komsebenzi
Ugcino kunye nobomi beShelf
-Le khithi ingagcinwa kwiinyanga ezili-12 phantsi kweemeko ezomileyo kwindawo yokushisa (15-25 ° C), ukuba ifuna ukugcinwa ixesha elide, inokugcinwa kwi-2-8 ° C.
Qaphela: Ukuba igcinwe kwiqondo lobushushu eliphantsi, isisombululo sithande ukuba nemvula.Ngaphambi kokusetyenziswa, qiniseka ukuba ubeke isisombululo kwikhithi kwindawo yokushisa kwegumbi ixesha elithile.Ukuba kuyimfuneko, yitshise kwindawo yokuhlambela amanzi angama-37 ° C imizuzu eyi-10 ukutshabalalisa i-precipitate, kwaye udibanise ngaphambi kokusetyenziswa.
-Isisombululo seForegene Protease sinefomula ekhethekileyo, esebenzayo xa igcinwe kwindawo yokushisa ixesha elide (iinyanga ezi-3);umsebenzi wayo kunye nokuzinza kuya kuba ngcono xa igcinwe kwi-4 ° C, ngoko kuyacetyiswa ukuba igcinwe kwi-4 ° C, khumbula ukuba ungayigcini -20 ° C.
-Ipowder eyomileyo yeLysozyme igcinwe kwi -20 ° C;isisombululo seLysozyme esilungisiweyo sahlulwe sibe ngamacandelo amancinci kwaye sigcinwe kwi -20 ° C.
