Utsalo lwe-Nucleic acid sisakhono esiyintshayelelo semifuniselo yebhayoloji yemolekyuli.Nokuba iyakwazi ukugqwesa kwaye isebenzise obu buchule ngobuchule ikwayinto efunekayo kubavavanyi bamva baphucule ukuya kwiinkosi zovavanyo.
Ngokwezixhobo ezahlukeneyo zophando, abafuni bahlulahlulwe ikakhulu kwezi ntlobo zintathu zilandelayo:
Ubukhulu bezilwanyana, Ubukhulu bezityalo, iMicrobiology enkulu
Uhlobo ngalunye lomfuniselo luya kuqhubeka nokukhuphela iikopi ukuhlakulela iseti yezakhono ezikhethekileyo kwiisampuli ezithile.
Namhlanje, umhleli ufuna ukuzisa izixhobo kunye neeprops ezingafumanekiyo ngentolongo kubavavanyi.Ngabo, unokuphucula ngokukhawuleza kuwo onke amacala.Asilophupha ukuba nguthixo wokulinga.
Lo mba ngowovavanyo lobuchwephesha bezityalo (abavavanyi bezinye iingcali banokufunda kuwo ~).
Ndiyakholelwa ukuba sonke isizukulwana sabavavanyi siya kuba sivile ngeendlela zokutsalwa kwezityalo ze-nucleic acid ezidluliswa ukusuka kwisizukulwana ukuya kwesinye ukusuka kwabo bandulelayo.Amagama amathathu amakhulu kwishishini lokutsalwa kwezityalo.
Nangona abafana abakhulu bebalasele, basenazo iintsilelo ezingenakuthintelwa.
Indlela ye-TRIzol
Idla ngokusetyenziswa ukukhupha
I-RNA
● Inetyhefu kwaye inzima
●Iqulathe ungcoliseko lwe-genome
Indlela yeCTAB
Idla ngokusetyenziswa ukukhupha
igenomeDNA
●Kutya ixesha
● Kunzima kakhulu ukususa i-CTAB ngokuhlamba kunetyuwa, intsalela encinci iya kuba nefuthe elikhulu kumsebenzi we-enzyme.
Indlela ye-SDS
Idla ngokusetyenziswa ukukhupha
iplasmid
●Ayizifanelanga izityalo ezinepolysaccharide ephezulu
●Usulelo lweprotheyini
Kulo luntu lwanamhlanje olunokuhlaziywa kwesantya esiphindwe kabini, iindlela zemveli zokusebenza azisakwazi ukuhlangabezana neemfuno zabavavanyi, kwaye ukuphuculwa kwezixhobo kusondele.
I-Foregene Nucleic Acid Extraction Kit iye yavavanywa amaxesha angama-N ngamaninzi abavavanyi.Kulula ukuyisebenzisa, ayisebenzisi nayiphi na i-reagents enetyhefu, kwaye ayifaki nayiphi na i-enzymes.I-asidi ye-nucleic efunyenweyo yococeko oluphezulu, oluyimfuneko ekusebenzeni kwemihla ngemihla yokutsalwa kwabavavanyi abagqwesileyo.
Foregene RNA Isolation kit
Apha, ndiya kuphinda ndibonise indlela yokusebenza efihliweyo kubavavanyi, okukukhetha indlela efanelekileyo yokukhutshwa ngokohlobo lwesityalo (indlela yokumisela uhlobo lwesityalo ekupheleni kwenqaku!), Kuba ezinye iipolysaccharides zezityalo kunye ne-polyphenols ziphezulu kumxholo, kwaye iindlela zemveli zibulalayo.Akwanelanga ukulawula.
Ewe, kunje ...
Indlela ye-TRIzol iguqe ngokuthe ngqo phantsi kweesampuli ezinamazinga aphezulu e-polysaccharides kunye ne-polyphenols ezifana namaqabunga ebhanana, amaqabunga e-ginkgo kunye namaqabunga ekotoni.
Ngale njongo, i-Foregene iphuhlise izixhobo ezibini ezisebenzayo zabavavanyi.Isixhobo sokutsalwa kwesityalo se-RNA esine-polysaccharide ephantsi kunye nomxholo we-polyphenol kunye ne-polysaccharide ephezulu kunye nomxholo we-polyphenol unokubulala iintshaba ngendlela ejoliswe kuyo, ukwenzela ukuba abahloli bangasazoyiki izityalo ezahlukeneyo.Guqula nje izixhobo ngokuthe ngqo!
Ukongeza kwimfuno yokuphucula indlela yokukhupha, isampuli yangaphambili yokucubungula imisebenzi nayo kufuneka iphuculwe.
Ngenxa yolwakhiwo olukhethekileyo lweesampulu zezityalo, iingcambu kunye neziqu eziqinileyo zezinye izityalo kunzima kakhulu ukugawula nokutyumza ngokulinganayo.
Kushishino lokusila, malunga nama-80% abantu basebenzisa initrogen engamanzi xa becola, ngelixa abangaphantsi kwe-20% basebenzisa ukucola kombane kunye nokusila i-bead oscillation.
Nangona ukucola initrogen yolwelo kutshiphu kwaye kulula ukuyisebenzisa, liphupha elibi kubavavanyi abanemfuno enkulu yesampulu yokugaya ixesha ngalinye kunye nomzamo!Ukuba udaka alucocwa, luya kubangela ukungcoliseka phakathi kweesampuli.
Umphumo wokugaya umbane uhluka ukusuka kumntu ukuya kumntu, kwaye imikhwa yokusebenza yabavavanyi iya kuba nempembelelo enkulu kwingqibelelo ye-nucleic acid, kunye nokusebenza okuphantsi kweqondo lokushisa kuyadingeka ukuba kuyimfuneko.
ke, ukusila bead oscillation kunokuthathwa njengento yangoku elula kwaye esebenzayo izixhobo zokusila.
Iindlela eziqhelekileyo ze-oscillation ziquka i-oscillation ethe nkqo, i-horizontal reciprocating oscillation kunye ne-three-dimensional (i-horizontal oscillation).Nangona kunjalo, i-Foregene's tissue crusher iqhekeza imodeli ekhoyo kwaye yamkele indlela entsha ye-4D ye-oscillation ukwenza ukucola okufanayo.
Ukuhlangabezana neemfuno zeesampulu ezahlukeneyo, kulula ukusebenza
Itekhnoloji eyohlukileyo ye-4D yokutyumza, ukusebenza kakuhle kokutyumza kunyuswe ngama-20%
Isampulu ivalwe ngokupheleleyo, akukho kungcoliseka komnqamlezo
Ukuzola, ≤56 iidecibels
Thelekisa iziphumo zovavanyo kunye namaza ~
Isampulu yovavanyo: 50mg amagqabi engqolowa (umxholo ophantsi we-polysaccharides kunye ne-polyphenols)
Khipha uhlobo lwe-nucleic acid: i-RNA
Isixa sendawo: 5μl
Ikhithi yokutsalwa esetyenzisiweyo yiForegene Plant Total RNA Extraction Kit (RE-05011)
Ukuthelekisa idatha kunye negrafu ye-electrophoresis, kunokubonwa ukuba ukugxininiswa kunye nokucoceka kwe-RNA ekhutshwe emva kokuba umatshini wokusila uphezulu kune-nitrogen yolwelo lokugaya.Ngelo xesha, ngenxa yokuba umatshini wokugaya unokwaphula isampuli ngokupheleleyo, i-RNA encinci inokukhutshwa kangcono kwaye ingqibelelo ye-RNA inokugcinwa.
Isampulu yovavanyo: 50mg amagqabi eginkgo (ipolysaccharide ephezulu kunye nomxholo wepolyphenol)
Khipha uhlobo lwe-nucleic acid: i-RNA
Isixa sendawo: 5μl
Ukusebenzisa iForegene Polysaccharide Polyphenol Plant Total RNA Extraction Kit (RE-05021)
Jonga kweli qela leisampulu ezinomxholo ophezulu wepolysaccharides kunye neepolyphenols.Isiphumo sibonakala ngakumbi.Ngobunyulu obufanayo, ukuxinana komatshini wokugaya i-RNA iphantse yaphinda ka-5 yolwelo lwenitrogen yokusila, kwaye iRNA encinci isabonakala kancinci ~
Ukuba uvakalelwa kukuba akukho nasinye kwezi zixhobo zingentla ngokukhawuleza ngokwaneleyo, ngoko umhleli kufuneka eze nesixhobo sokugqibela!
Tyala PCR ngqo series
Isici esikhethekileyo sesi sixhobo kukuba isityalo amaqabunga okanye imbewu akufuneki ukuba ibe phantsi kwaye yaphukile, kwaye iphoswe ngokuthe ngqo kwi-tube ye-EP ye-lysis, kwaye umxube we-lysis obangelwayo ungasetyenziswa njenge-template ye-PCR reaction.Ilula njengenyathelo elinye ngokukhawuleza!Kwangaxeshanye, uthotho lwePCR oluthe ngqo lweZityalo zeForegene zixhotyiswe ngenkqubo ye-UNG yokuchasana nokungcoliseka, ukuze abavavanyi bangabi sazikhathaza ngongcoliseko lwe-aerosol kwilabhoratri kwaye banokuluthatha ngokulula uvavanyo.
Iingcebiso zesigwebo:
Indlela yokugweba ukuba ngaba sisityalo se-polysaccharide polyphenol
Thatha malunga ne-20mg ibhloko ye-tissue kwaye uyifake kwi-tube ecocekileyo ye-1.5ml ye-centrifuge, yongeza i-200ul 200mM isisombululo se-NaOH, uyibeke kwi-95 ℃ ye-10min, qaphela utshintsho lombala emva kwe-lysis.
1. Ukuba umbala we-lysate uluhlaza okanye ophuzi okhanyayo, oko kuthetha ukuba umxholo we-tissue polyphenols uphantsi, kwaye kucetyiswa ukuba kusetyenziswe uchungechunge lwe-Foregene Plant.
2. Ukuba i-lysate i-brownish-yellow okanye i-brownish-red, ithetha ukuba umxholo we-polyphenol we-tissue uphezulu, kwaye kucetyiswa ukuba usebenzise i-Foregene Polysaccharide Polyphenol Plant Plus Series.
Ixesha lokuposa: Aug-20-2021
