Yintoni iDirectPCR?
I-teknoloji ye-DirectPCR ithetha ukuba ngaphandle kokwahlula kunye nokucoca i-nucleic acid molecules (kubandakanya i-DNA kunye ne-RNA) yeesampuli zezicubu ezahlukeneyo, kuphela i-tissue kunye nesakhiwo seseli sichithwa yi-lysis ye-protease, i-nucleic acid ikhutshwa kwisisombululo se-lysis, kwaye isisombululo se-lysis songezwa ngokuthe ngqo.Inkqubo yokusabela kwe-PCR bubuchwepheshe bokukhulisa ijini ekujoliswe kuyo.
Ukukhangela utshintsho
Ukwahlula kunye nokukhutshwa kwe-nucleic acids umcimbi
Ukususela ekuqulunqweni kweteknoloji ye-PCR kwiminyaka eyi-30 edlulileyo, abaphandi baye bakhathazeka ngokuhlukana kunye nokukhutshwa kwe-nucleic acids.Ukusebenzisa iisampulu zethishu ukwenza ngokuthe ngqo ukuphendula kwe-PCR liphupha labaphandi abaninzi.Kodwa eli phupha alizange lizaliseke iminyaka engama-30.Isizathu kukuba i-tissue edibeneyo iya kukhulula ezininzi izithako zokuthintela.
Ezi zixhobo zokuthintela ziya kuba nefuthe elinamandla lokuthintela kwi-PCR reaction, ezinye ziya kubangela ukuba i-primer kunye nethemplate ingakwazi ukubopha, abanye banomsebenzi oqinileyo we-protein denaturation, okubangela ukungasebenzi kwe-nucleic acid polymerase, kwaye ezinye zithintela ngokuthe ngqo i-primer kunye ne-template ekubopheni.Ezi zizinto zonke ezibangela ukuba ukuphendula kwe-PCR kungahambi kakuhle.
Ngokwemveli, abantu ngamanye amaxesha basebenzisa izixhasi ze-PCR ukomeleza ubuntununtunu, ukuchaneka kunye nokwandiswa kobuchule bokusabela kwe-PCR.Nangona kunjalo, ngenxa yee-PCR inhibitors ezahlukeneyo eziqulethwe kwiitemplates ze-nucleic acid ezivela kwimithombo eyahlukeneyo, ukusetyenziswa kwexesha elide lenani elikhulu lezixhasi ngokucacileyo akufanelekanga ilahleko, ixabiso liphezulu, kwaye umsebenzi unzima.
I-DirectPCR yangaphambili
Impumelelo ephambili kwihlabathi --Iindlela ezimbini
I-Foregene yenze impumelelo kweli candelo letekhnoloji kwinqanaba lehlabathi.I-teknoloji ye-Foregene DirectPCR inamanqaku amabini obugcisa, okwenza ukuba i-Foregene ibe nguvulindlela kunye nenkokeli ye-teknoloji ye-DirectPCR.
Okokuqala, i-patented nucleic acid polymerase modification method.I-Forgene ine-patented nuclease modification method, ejoliswe kwi-domain ebophayo ukwenza i-nuclease kunye ne-template ibe namandla, kunye nomsebenzi ophezulu, ukusebenza kakuhle kokukhulisa kunye nokuzinza kwe-thermal.
Okwesibini, ifomula yePCR Mix elungiselelwe iintlobo ezahlukeneyo, izixhasi zokusabela ezikhethekileyo, izilungisi kunye nezizinzisi, ziphucula kakhulu ukuxhathisa kwepolymerase kwi-PCR inhibitors kunye nokuqinisekisa ukusebenza kakuhle kokukhulisa.
Kungenxa kanye yezi zinto zikhankanywe ngasentla ezimbini ezibalulekileyo zetekhnoloji apho i-Foregene ifumene i-DirectPCR yokwenyani kwinqanaba lehlabathi.Nokuba zizilwanyana eziqhelekileyo zezilwanyana, ulwelo lomzimba, izicubu zezityalo, amagqabi okanye iingcebiso zeengcambu, okanye nembewu yezityalo, abasebenzisi banokufikelela ngokulula kwi-PCR yokukhulisa ngokuthe ngqo ngaphandle kokuqhekeka komatshini okanye inkqubo yokucocwa kwe-nucleic acid edinayo.
Kwiikiti ze-DirectPCR zoluhlu lwezityalo kunye nochungechunge lwezilwanyana eziye zaqaliswa, i-Foregene inokuthetha ngokuzingca ukuba sinezikhombisi ezihamba phambili zehlabathi.Kwixesha elizayo, i-Foregene iya kuqhubeka nokusungula uluhlu lweemveliso ezisenazo izikhombisi zokusebenza ezihamba phambili zehlabathi (okanye zihluke).
Ngolwazi oluthe vetshe, ngenisa:
http://www.foregene.com/http://www.foreivd.com/
Ixesha lokuposa: Feb-27-2017
