I-Hot-start Taq enzyme isetyenziswa ngokubanzi.Xa kuthelekiswa ne-polymerase ye-DNA eqhelekileyo, i-enzyme ye-Taq eshushu iqala ngokufanelekileyo ikwazi ukuphepha ukukhuliswa kwe-non-specfic kunye nokwakhiwa kwe-primer dimers, kwaye inokuphucula ngokufanelekileyo izinga lokuphumelela kwe-target gene amplification.Ngokukodwa kwinkalo yovavanyo lwemfuza, i-enzyme ye-Taq eshushu iqale ichongiwe njengomgangatho onyanzelekileyo kwishishini, kwaye i-polymerase ye-DNA eqhelekileyo ayifanele isetyenziswe.Njengoko kunokubonwa apha ngasentla, ii-enzyme ze-Taq ezishushu zisetyenziswa ngokubanzi.Okwangoku, zininzi iibrendi ze-Taq eshushu eziqalayo kwimarike yasekhaya, kodwa azikho zininzi ii-enzymes ze-Taq ezinomgangatho ophezulu.Sijongene neemveliso ezininzi ze-enzyme ye-Taq eshushu, kufuneka sikhethe njani?

1. Khetha i-enzyme ye-Taq eshushu-yokuqala ngokusebenza kakuhle kokukhulisa

Ukusebenza kokukhulisa i-PCR kuhambelana ngokusondeleyo nokusebenza kwe-Taq enzyme.Emva kokuba inkqubo yempendulo ye-enzyme ye-Taq iphuculwe, ukusebenza kakuhle kokukhulisa kungaphezulu kwe-95%, kwaye uluhlu lokukhulisa isixa sokuqala setemplate sibanzi.Ukwandiswa okwanelisayo kunokufumaneka xa umxholo we-gene ekujoliswe kuwo uphantsi, kwaye akulula ukuba ube netyhefu xa isixa setemplate siphezulu, kwaye ixesha lokukhulisa i-exponential lide.Kwi-enzyme ye-Taq engasebenzi kakuhle, nokuba inkqubo yokusabela iye yaphuculwa amaxesha amaninzi, ukusebenza kakuhle kokukhulisa kusengaphantsi kwe-90%, imilo ye-"S" ye-curve yokukhulisa ayibonakali, i-slope incinci, kwaye ijika lithe tyaba.Xa inani le template liphantsi, alikwazi ukunyuswa, kwaye xa inani le template liphezulu, umphumo wokukhulisa awufanelekanga.Ke ngoko, ukukhethwa kweepolymerasi ze-DNA ezinokwandiswa okuphezulu kobuchule kubalulekile kwimpumelelo ye-PCR kunye ne-qPCR.

2. Khetha i-enzyme ye-Taq eshushu-yokuqala ngamandla anamandla e-enzyme

Amandla e-enzymatic ye-Taq enzyme anxulumene nokusebenza kakuhle kokukhulisa.Ngokubanzi, okukhona omelele amandla e-enzymatic ye-enzayim ye-Taq eshushu, kokukhona ixesha lokukhula kwe-PCR lide, kokukhona ijiko eliqhelekileyo 'elimile okwe-S', kokukhona liphezulu ixabiso lophawu lwe-fluorescence, kunye nokulunge ngakumbi ukufunyaniswa kwe-multiplex PCR.Iipolymerasi zeBrand DNA ezinamandla abuthathaka enzymatic zinokuxhasa kuphela ii-2-plex reactions.Xa usenza i-3-plex reactions, i-curve yokukhulisa iphantsi, ixabiso lesignali ye-fluorescence liphantsi, kwaye akukho jiko lokukhulisa eliqhelekileyo, ngoko ke iziphumo kunzima ukuzigweba.

3. Khetha i-enzyme ye-Taq eshushu-yokuqala enovakalelo oluphezulu

Ngokuqhelekileyo, i-polymerase ye-DNA ine-amplification ephezulu kunye novakalelo oluphezulu, kodwa kukho ukungahambelani.Ukuba ubuninzi bemfuza ekujoliswe kuyo kwisampulu ekufuneka ikhuliswe iphantsi, kuyacetyiswa ukuba kuvavanywe uvakalelo lwe-amplification ye-Taq enzyme.Eyona ndlela ixhaphakileyo yokufumanisa kukuqhuba i-10-fold okanye i-5-fold gradient dilution ye-target gene fragment plasmid, uqhube ubhaqo lwe-PCR kwi-dilution esezantsi, kwaye ukhethe i-enzyme ye-Taq eshushu enovakalelo oluphezulu.

Kunokubonwa koku kungasentla ukuba abaphandi kufuneka bakhethe ngokweemfuno zabo zovavanyo kunye neemeko zenkxaso-mali.Kungcono ukwenza umfuniselo wokukhulisa i-gradient dilution ukubona impumelelo yokukhulisa kunye novakalelo lwe-enzayimi ye-Taq eshushu.

Umzekelo weForegene's Taq DNA Polymerase:

Foreasy HS Taq DNA Polymerase

Inkcazo

I-Foreasy HS Taq DNA Polymerase yipolymerase ye-DNA echazwe kwi-Escherichia coli iibhaktheriya zobunjineli ngeteknoloji yokuhlanganiswa kwakhona kwemfuza.I-enzyme idibaniswe ne-buffffer yokusabela ekhethekileyo, eyenza imveliso ixhathise kakhulu kwaye ihambelane, kwaye ingasebenzisa ngokuthe ngqo isampuli ye-lysate (inkqubo ye-Forgene Lysis) njenge template yokujonga ukuphendula.

Isicelo

I-PCR esemgangathweni kunye nokufumanisa i-PCR yobuninzi beetemplate ezihlambulukileyo kunye neetemplates ezingahlanjululwanga.

Ulawulo lwemeko

1.Akukho msebenzi wangaphandle we-nuclease ufunyenweyo

Indlela ye-2.PCR yokufumanisa i-DNA ye-genomic eseleyo ngaphandle komninimzi

3.Inokukhulisa ngokufanelekileyo ikopi enye yemfuza kwiGenome yomntu

4.Gcina kwiqondo lobushushu begumbi kangangeveki,utshintsho lomsebenzi ocacileyo

Iinkcukacha zeMveliso: https://www.foreivd.com/foreasy-hs-taq-dna-polymerase-product/