Iindaba zoShishino
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Isishwankathelo senqaku-i-RNase inhibitors
Ababhali: Wang Xiaoyan, iYunithi ye-Zhao Eryu: Isibhedlele saseJiaozhou, iSibhedlele sase-Dongfang esiNxulumene neYunivesithi yaseTongji Okwangoku, uhlobo oluphambili lwesampulu yokufumanisa i-pathogen ye-nucleic acid yokuphefumla yi-swab yomqala.Kukho iintlobo ezininzi zezisombululo zokugcinwa kwesampulu ezisetyenziswa ngokuqhelekileyo, zonke kufuneka zibe...Funda ngokugqithisileyo -
Qonda i-RT-qPCR kwinqaku elinye
Izixhobo zokuqalisa: I-RNA Ubungakanani bombhalo obubuyisela umva i-PCR (RT-qPCR) yindlela yovavanyo esetyenziswa kwimifuniselo ye-PCR kusetyenziswa i-RNA njengesixhobo sokuqala.Kule ndlela, i-RNA iyonke okanye i-RNA yesithunywa (mRNA) ibhalwa kuqala kwi-DNA ehambelanayo (cDNA) nge-reverse transcriptase.Emva koko...Funda ngokugqithisileyo -
Fluorescence quantitative PCR
W: Mzalwana, ukukhutshelwa umva kugqityiwe, kwaye ekugqibeleni inyathelo lokugqibela - i-fluorescent quantitative PCR!Hee, mzalwana, yintoni i-PCR yexesha lokwenyani?M:PCR uhlala uyazi, akunjalo?W: Uyazi, i-PCR yi-polymerase chain reaction, kwaye iteknoloji eyandisa i-DNA!Mna: Ewe...Funda ngokugqithisileyo -
Ukulungiswa kokutsalwa kwe-RNA kunye nezilumkiso
I-Sterrilization yeengcebiso ze-pipette kunye neetyhubhu ze-EP, njl. 1. Lungiselela i-0.1% (iwaka) i-DEPC (into enetyhefu kakhulu) ngamanzi adibeneyo, ayisebenzise ngononophelo kwi-fume hood, kwaye uyigcine kwi-4 ° C kude nokukhanya;Amanzi e-DEPC ngamanzi acocekileyo aphathwa nge-DEPC kwaye acociweyo bubushushu obuphezulu kunye noxinzelelo oluphezulu...Funda ngokugqithisileyo -
Ungayikhetha njani iTaq/DNA polymerase eshushu
I-Hot-start Taq enzyme isetyenziswa ngokubanzi.Xa kuthelekiswa ne-polymerase ye-DNA eqhelekileyo, i-enzyme ye-Taq eshushu iqala ngokufanelekileyo ikwazi ukuphepha ukukhuliswa kwe-non-specfic kunye nokwakhiwa kwe-primer dimers, kwaye inokuphucula ngokufanelekileyo izinga lokuphumelela kwe-target gene amplification.Ngokukodwa kwicandelo lemfuzo ...Funda ngokugqithisileyo -
Ithemba loluntu!Funda isitofu sokugonya se-mRNA kwinqaku elinye
KwiNkomfa yeSitofu kunye nezeMpilo, iingcali zabiza "wonke umntu kufuneka anikele ingqalelo kugonyo lwe-mRNA, olunika abantu ukucinga okungenamkhawulo."Ke yintoni kanye kanye isitofu sokugonya se-mRNA?Yafunyaniswa njani kwaye lithini ixabiso lesicelo sayo?Ngaba inokumelana neC...Funda ngokugqithisileyo -
Ii-reagents ezahlukeneyo zakha i-backing eqinileyo-i-Forgene Biotech inceda ukufumanisa intsholongwane yemonkeypox
Ubume bangoku bentsholongwane yemonkeypox Ngokutsho kweendaba ze-WHO ngoJuni 7, kwiiveki nje ezintathu (ngoMeyi 13 ukuya kuJuni 7), ngaphezu kwe-1,000 yeemeko zemonkeypox ziye zaxelwa kumazwe angama-27 angenayo i-monkeypox-endemic kunye nemimandla.I-WHO ikholelwa ukuba iimeko zinokujongelwa phantsi ngenxa ye-epid encinci ...Funda ngokugqithisileyo -
Iimpendulo kwimibuzo eli-11 oyikhathaleleyo kakhulu malunga nerhashalala
Ngokweengxelo ezibanzi, ukuza kuthi ga ngoku, usulelo lwemonkeypox luye lwanwenwela kumazwe ali-15 angaphandle kweAfrika, nto leyo evuselela umdla kunye nenkxalabo evela kumazwe angaphandle.Ngaba intsholongwane yemonkeypox ingatshintsha?Ngaba kuya kubakho ukuqhambuka okukhulu?Ngaba iyeza lokugonya ingqakaqha lisasebenza...Funda ngokugqithisileyo -
IiPipette zoMbane ze-ForePipet, Zisa uVavanyo lwamava amatsha!
Ukusukela oko u-Schnitger, isazinzulu kwiZiko laseJamani lePhysiology kunye neKhemistry, wavelisa i-micropipette yokuqala yehlabathi (pipette) ngo-1956, i-pipette iye yahamba phantse iminyaka engama-70 yophuhliso, ukusuka kumlinganiselo ukuya kwi-adjustable, ukusuka kwitshaneli enye ukuya kwi-Multichannel ine...Funda ngokugqithisileyo -
Into ekufuneka uyazi malunga neentlobo ezahlukeneyo
I-Omicron Variant: Yintoni ekufuneka Uyazi Ulwazi malunga neZintlukwano: Iintsholongwane zihlala zitshintsha ngotshintsho kwaye ngamanye amaxesha olu tshintsho lukhokelela kulunye uhlobo olutsha lwentsholongwane.Ezinye iinguqulelo ziyavela kwaye zinyamalale ngelixa ezinye ziqhubeka.Ukwahluka okutsha kuya kuqhubeka kuvela.I-CDC kunye nezinye iindaba zoluntu ...Funda ngokugqithisileyo -
I-R&D |Ubume bangoku bophuhliso lwemveliso yeziyobisi ze-nucleic acid
Ngophuhliso oluqhubekayo lwetekhnoloji yebhayoloji yemolekyuli, ubudlelwane phakathi kokuguqulwa kofuzo kunye neziphene kunye nezifo ziye zafumana ukuqonda okunzulu ngakumbi.Ii-Nucleic acids zitsale ingqalelo enkulu ngenxa yamandla azo amakhulu okusetyenziswa kuxilongo kunye ne…Funda ngokugqithisileyo -
Malunga nama-antibodies e-monoclonal, inqaku elilungileyo ongenakuliphosa!
Ii-antibodies, ezikwabizwa ngokuba yi-immunoglobulins (Ig), ziyi-glycoprotein ezibophelela ngokukodwa kwii-antigens.Ukulungiswa kwe-antibody eqhelekileyo kuveliswa ngokugonywa kwezilwanyana kunye nokuqokelela i-antiserum.Ke ngoko, i-antiserum ihlala iqulethe izilwa-buhlungu ezichasene nezinye ii-antigens ezinganxulumananga kunye nezinye iiproteni c...Funda ngokugqithisileyo
