Ukubambelela kwimbono "yokwenza iimpahla ezikumgangatho ophezulu kunye nokwenza abahlobo abalungileyo nabantu namhlanje abavela kwihlabathi liphela", sihlala sibeka umdla wabathengi ukuba siqale ngo-2019 Umgangatho oPhezulu weZixhobo zezoNyango zaseTshayina eziphathwayo ngexesha lokwenyani lePCR System DNA/Rna Extraction Kit, Siza kuxhobisa abantu ngokunxibelelana kunye nokuphulaphula nokufunda kumzekelo ukuya kwabanye.
Ukubambelela kwimbono "yokwenza iimpahla ezikumgangatho ophezulu kunye nokwenza ubuhlobo obuhle nabantu namhlanje abavela kwihlabathi liphela", sihlala sibeka umdla wabathengi ukuqala ngawo.China Nucleic Acid Extraction, Nucleic Acid Extraction Kit, Sigxininisa "kwiMgangatho wokuQala, uDumo lokuqala kunye noMthengi wokuqala".Sizimisele ukubonelela ngeemveliso ezikumgangatho ophezulu kunye neenkonzo ezilungileyo emva kokuthengisa.Ukuza kuthi ga ngoku, iimveliso zethu ziye zathunyelwa kumazwe angaphezu kwama-60 kunye nemimandla ehlabathini lonke, ezifana America, Australia kunye neYurophu.Siyakonwabela udumo oluphezulu ekhaya naphesheya.Sihlala sizingisa kumgaqo "weTyala, uMthengi kunye noMgangatho", silindele intsebenziswano nabantu kuzo zonke iinkalo zobomi ukuze bafumane inzuzo efanayo.

Iinkcukacha

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Ikiti isebenzisa ikholomu ye-spin kunye nefomula ephuhliswe yi-Foregene, enokuthi ikhuphe ngokufanelekileyo ubunyulu obuphezulu kunye nomgangatho ophezulu we-RNA kwiintlobo ezahlukeneyo zezityalo ezine-polysaccharides eziphantsi kunye nomxholo we-polyphenols.Kwiisampuli zezityalo ezine-polysaccharides eziphezulu okanye umxholo we-polyphenols, kuyacetyiswa ukuba usebenzise i-Plant Total RNA Isolation Plus Kit ukuze ufumane iziphumo ezingcono ze-RNA zokukhutshwa.Ikiti inikezela nge-DNA-Cleaning column enokuthi isuse ngokulula i-DNA ye-genomic kwi-supernatant kunye ne-tissue lysate.Ikholamu ye-RNA kuphela inokubopha ngokufanelekileyo i-RNA.Ikhithi inokuqhuba inani elikhulu leesampuli ngexesha elinye.

Inkqubo yonke ayinayo i-RNase, ngoko ke i-RNA ecocekileyo ayiyi kuthotywa.I-Buffer PRW1 kunye ne-Buffer PRW2 inokuqinisekisa ukuba i-RNA efunyenweyo ayingcoliswanga yiprotheni, i-DNA, i-ion, kunye ne-organic compounds.

Amacandelo ekhithi

Iimpawu&izinto eziluncedo

■ Ukusebenza kwiqondo lobushushu begumbi (15-25℃) kuyo yonke inkqubo, ngaphandle kokuhlamba komkhenkce kunye nobushushu obuphantsi be-centrifugation.
■ Gqibezela ikhithi ye-RNase-Free, akukho mfuneko yakukhathazeka malunga nokuthotywa kwe-RNA.
∎ IKholam yokucoca i-DNA ibophelela ngokukodwa kwi-DNA, ukuze ikhithi ikwazi ukususa ungcoliseko lwe-genomic DNA ngaphandle kokongeza i-DNase.
■ Isivuno esiphezulu se-RNA: IKholamu ye-RNA kuphela kunye nefomula ekhethekileyo inokucoca ngokufanelekileyo i-RNA.
■ Isantya esikhawulezayo: kulula ukusebenza kwaye sinokugqitywa kwimizuzu engama-30.
■ Ukhuseleko: akukho sixhobo sokwenza izinto eziphilayo esifunekayo.
∎ Umgangatho ophezulu: Amaqhekeza e-RNA acociweyo anobunyulu obuphezulu, awanaprotheyini kunye nobunye ukungcola, kwaye anokumelana nemifuniselo eyahlukahlukeneyo esezantsi komlambo.

Isicelo seKit

Ifanelekile ukutsalwa kunye nokucocwa kwe-RNA iyonke kwiisampuli zezicubu zesityalo esitsha okanye enomkhenkce (ingakumbi izicubu zeqabunga lesityalo esitsha) kunye ne-polysaccharide ephantsi kunye nomxholo we-polyphenol.

Ukuhamba komsebenzi

Umzobo

Isityalo esipheleleyo se-RNA Isolation Kit Plus icutshungulwe i-50mg yamagqabi amatsha e-polysaccharides kunye ne-polyphenols, kunye ne-5% ecocekileyo ye-RNA yavavanywa yi-electrophoresis.
1: Ibhanana
2: Ginkgo
3: Umqhaphu
4: Irharnati

Ukugcinwa kunye nobomi beshelufu

Ikhithi ingagcinwa iinyanga ezili-12 kwiqondo lobushushu begumbi (15–25 ℃) kwindawo eyomileyo, kunye ne-2–8 ℃ ixesha elide (iinyanga ezingama-24).

I-Buffer PSL1 inokugcinwa kwi-4 ℃ kwinyanga eyi-1 emva kokongeza i-2-hydroxy-1-ethanethiol (ukhetho) .Ukunamathela kwimbono "Ukudala iimpahla ezisemgangathweni ophezulu kunye nokwenza ubuhlobo obuhle nabantu namhlanje abavela kwihlabathi lonke", sihlala sibeka umdla wabathengi ukuqala ngo-2019 umgangatho ophezulu we-China Medical Equipment / PCnaRing System yokukhupha amandla kwi-PCR ye-Telkom ye-Real. ukunxibelelana nokuphulaphula, Ukwenza umzekelo kwabanye nokufunda kumava.
2019 Umgangatho ophezuluChina Nucleic Acid Extraction, Nucleic Acid Extraction Kit, Sigxininisa "kwiMgangatho wokuQala, uDumo lokuqala kunye noMthengi wokuqala".Sizimisele ukubonelela ngeemveliso ezikumgangatho ophezulu kunye neenkonzo ezilungileyo emva kokuthengisa.Ukuza kuthi ga ngoku, iimveliso zethu ziye zathunyelwa kumazwe angaphezu kwama-60 kunye nemimandla ehlabathini lonke, ezifana America, Australia kunye neYurophu.Siyakonwabela udumo oluphezulu ekhaya naphesheya.Sihlala sizingisa kumgaqo "weTyala, uMthengi kunye noMgangatho", silindele intsebenziswano nabantu kuzo zonke iinkalo zobomi ukuze bafumane inzuzo efanayo.